LOCUS       sequence-246080-        8687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Zhao B, Li L, Lu Q, Wang LH, Liu CY, Lei Q, Guan KL
  TITLE     Angiomotin is a novel Hippo pathway component that inhibits YAP 
            oncoprotein.
  JOURNAL   Genes Dev. 2011 Jan 1;25(1):51-63.
  PUBMED    21205866
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2011-01-1"; volume: "25(1)"; pages: "51-63"
COMMENT     Sequence Label: HA-AMOT p130
FEATURES             Location/Qualifiers
     source          1..8687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..32
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(151..171)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        156..485
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      336..471
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     398..417
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             552..1346
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(606..625)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1216..1235
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1520..1641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1557..1576)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1611..1630
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1690..1706)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1690..1706)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1703..1725)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1714..1730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1738..1744,1745..1762,1763..1768))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1783..1804
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1921..1938)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2092..2680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2172..2191)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2851..3642,3643..3711))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3474..3493
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3712..3816)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3891..3910)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4082..4461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4462..4665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4616..4636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4710..4729
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4710..4728
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4751..4777
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4781..4807
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5966..5986
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             7516..7533
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(8087..8105)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8088..8105)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    8131..8355
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(8401..8687,1..142)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8488..8507)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
       61 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
      121 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
      181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      241 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      301 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      361 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      421 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      481 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
      541 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
      601 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
      661 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
      721 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
      781 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
      841 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
      901 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
      961 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     1021 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     1081 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     1141 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     1201 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     1261 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     1321 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     1381 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     1441 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     1501 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     1561 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1621 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     1681 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1741 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1801 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1861 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1921 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1981 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2041 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2101 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2161 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2221 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2281 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2341 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2401 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2461 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2521 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2581 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2641 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2701 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2761 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2821 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2881 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2941 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3001 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3061 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3121 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3181 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3241 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3301 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3361 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3421 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3481 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3541 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3601 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3661 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3721 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3781 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3841 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     3901 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     3961 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     4021 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     4081 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     4141 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4201 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4261 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4321 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4381 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4441 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     4501 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4561 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     4621 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
     4681 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
     4741 tgccatggcc tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga
     4801 ctatgcagga tccatgagaa attctgacga acagccaagt ggagggacca cggtattgca
     4861 gcgtttgcta caagagcagc ttcgctatgg caatcctagt gagaatcgca gcctgcttgc
     4921 catacaccag caagccacag ggaatggccc tcctttcccc agtggcagtg ggaacccggg
     4981 ccctcagagt gatgtgttga gtccccaaga ccaccaccaa cagcttgtgg ctcatgctgc
     5041 tcgacaagaa ccccaggggc aggaaatcca gtcagaaaac ctcatcatgg agaagcagct
     5101 gtctcctcga atgcaaaata atgaagaact cccgacctat gaagaagcca aggtccagtc
     5161 ccagtacttt cggggccaac agcatgccag tgttggagct gccttctatg tcactggagt
     5221 caccaaccag aagatgagga ctgagggacg cccatcagtt cagcggctca atcctggaaa
     5281 gatgcaccaa gatgagggac tcagagacct taagcaaggg catgtccgtt ccttgagtga
     5341 acgactaatg cagatgtcac tggccaccag tggagttaag gcccatccac ctgttaccag
     5401 tgctcccctc tccccaccac aacccaatga cctctacaag aatcccacaa gttccagtga
     5461 attctacaag gcccaagggc cacttcctaa ccagcatagc ctgaagggca tggaacaccg
     5521 aggcccccca ccagaatatc ccttcaaggg catgccaccc caatctgtag tgtgcaagcc
     5581 ccaagagcca gggcacttct atagtgagca tcgcctgaac cagccaggga gaacagaggg
     5641 gcaactgatg aggtatcagc atccccctga gtatggagca gccaggccag cgcaggacat
     5701 ctcattgcca ttgtcagcca ggaactcgca gcctcacagc cctacttctt ctctgacctc
     5761 tggggggtcc ttgcccttgc tacaatctcc accatccact agattgtctc ctgcccgaca
     5821 ccccttggtc ccaaaccagg gagaccattc agctcacctg cctaggccgc agcagcattt
     5881 ccttcctaat caggctcacc agggggatca ttaccgtctc tcccaacctg gcctgagtca
     5941 gcagcagcag caacagcagc agcagcacca tcatcaccat caccaccaac aacagcagca
     6001 gcagcagcca cagcagcagc caggagaagc ctattcagct atgcctcggg ctcagccatc
     6061 ctctgcttct tatcagccag tgccagcaga cccttttgcc attgtttcca gagcccagca
     6121 gatggttgag atcctctcag acgagaaccg gaacttgagg caagagttgg aaggatgcta
     6181 tgagaaggtg gcaagactgc agaaggtgga gacagaaatc cagcgcgtct cggaggcata
     6241 tgagaacctc gtgaagtcat cctccaaaag agaggcccta gagaaagcca tgagaaacaa
     6301 gctagagggc gagattcgga ggatgcatga tttcaacagg gatctgagag agcgtctaga
     6361 gactgccaac aagcagcttg cagagaagga atatgagggg tcagaggaca ccagaaaaac
     6421 catctcgcag ctctttgcaa aaaataaaga aagccagcgt gagaaggaga agctggaagc
     6481 ggagctggcc actgcccgtt ctaccaatga ggaccaaaga cgacacatcg aaatccgaga
     6541 tcaggccctg agtaatgccc aggccaaggt ggtaaagctg gaagaagagc tgaaaaagaa
     6601 gcaagtgtac gttgacaagg tggagaagat gcagcaggcc cttgtacagc tccaggcagc
     6661 atgtgaaaaa cgtgagcagc tagagcaccg tctccggaca cgactggaga gggaactgga
     6721 atccctgaga atccagcagc gtcagggcaa ctgtcagccc accaacgttt cagaatacaa
     6781 tgctgctgca ctgatggagc tccttcggga gaaagaggag aggattctgg ctctggaagc
     6841 tgatatgaca aagtgggagc agaaatattt ggaggagaat gtgatgagac attttgctct
     6901 ggatgctgct gcaactgtgg ctgctcagag ggacacaaca gtcatcagtc actctcctaa
     6961 caccagctat gacacagctc tagaagctcg catccagaaa gaggaggaag aaatcttgat
     7021 ggccaataag cgttgccttg acatggaggg caggattaag accctccatg cccagattat
     7081 tgagaaggat gccatgatca aagtactcca gcagcgttcc cggaaggagc cgagcaagac
     7141 agagcagctg tcgtgcatgc ggccagcgaa gtctctgatg tccatttcca atgctggatc
     7201 aggcttgctc tcccactcat ccaccctgac tggctccccc atcatggaag aaaagcgaga
     7261 cgacaagagc tggaagggga gcctaggcat tctcctgggt ggagactacc gtgctgaata
     7321 tgtcccttcc acaccctcgc ctgtgccacc ctcgactccc ctgctctcgg ctcactccaa
     7381 gacagacagc cgagactgca gtacccaaac tgaacgtggg acggaatcga acaaaactgc
     7441 agctgttgct cccatctctg ttcctgctcc agttgctgct gccgccactg ctgccgccat
     7501 cactgccact gctgccacca tcaccaccac catggtagct gctgctccag ttgctgttgc
     7561 tgctgctgct gctccagctg ctgctgctgc cccgtctcca gccactgccg ctgctactgc
     7621 tgctgctgtt tctccagctg ctgctggtca gattccagct gctgcctctg ttgcctcagc
     7681 tgctgccgtt gctccttctg ctgctgctgc tgctgctgtt caggttgctc cagctgctcc
     7741 ggctccagtt ccagctccgg ctctggttcc ggtcccagct ccagcagcgg ctcaggcttc
     7801 tgctcctgct cagactcagg caccaacttc agctccggct gtggctccaa ctccagctcc
     7861 aactccaact ccagctgtgg ctcaggctga ggttcctgca agtccagcta ccggtcctgg
     7921 accacatcgt ttgtctatac caagtttgac ctgcaatcca gacaaaacag atgggcctgt
     7981 gttccactcc aatactctgg aaagaaaaac tcccattcag atcctgggac aagagcctga
     8041 tgcagagatg gtggaatatc tcatctaact cgaggggggg ccctattcta tagtgtcacc
     8101 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     8161 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     8221 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     8281 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     8341 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     8401 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8461 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8521 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8581 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8641 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacg
//
