LOCUS       sequence-246098-        9619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing a GFP-tagged degron in the soma of C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Zhang L, Ward JD, Cheng Z, Dernburg AF
  TITLE     The auxin-inducible degradation (AID) system enables versatile 
            conditional protein depletion in C. elegans.
  JOURNAL   Development. 2015 Nov 9. pii: dev.129635.
  PUBMED    26552885
REFERENCE   2  (bases 1 to 9619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2015 Nov 9. pii: dev.129635."
COMMENT     Sequence Label: pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
FEATURES             Location/Qualifiers
     source          1..9619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1491..1502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3601..4200
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     CDS             4212..4343
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     primer_bind     7604..7626
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7664..7682)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7750..7854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7855..7923,7924..8715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8073..8092)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8886..9474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9375..9394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa
       61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg
      121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt
      181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta
      241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt
      301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta
      361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa
      421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc
      481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa
      541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg
      601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc
      661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat
      721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca
      781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca
      841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga
      901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata
      961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga
     1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat
     1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca
     1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac
     1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc
     1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat
     1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt
     1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct
     2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     3361 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac
     3481 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta ggtatctaga
     3541 accggtgacg tcaccatggg aagcttcgtg gatccagata tcctgcagga attcctcgag
     3601 gcacctttgg tcttttattg tcaacttcca ttggttcttc cattgtttct gttaaattaa
     3661 tgaatttttc ataaaataaa gacattatac aatataaaaa tgaagaattt attgaaaata
     3721 aactgccaga gagaaaaagt atgcaacact cccgccgaga gtgtttgaaa tggtgtacgg
     3781 tacattttcg tgctaggagt tagatgtgca ggcagcaacg agagggggag agattttttt
     3841 gggccttgtg aaattaacgt gagttttctg gtcatctgac taatcatgtt ggttttttgt
     3901 tggtttattt tgtttttatc tttgttttta tccagattag gaaatttaaa ttttatgaat
     3961 ttataatgag gtcaaacatt cagtcccagc gtttttcctg ttctcactgt ttagtcgaat
     4021 ttttatttta ggctttcaac aaatgttcta actgtcttat ttgtgacctc actttttata
     4081 tttttttaat ttttaaaaat attagaagtt tctaggataa ttttttcgac ttttattctc
     4141 tctaccgtcc gcactcttct tacttttaaa ttaaattgtt tttttttcag ttgggaaaca
     4201 ctttgctcat gcctaaagat ccagccaaac ctccggccaa ggcacaagtt gtgggatggc
     4261 caccggtgag atcataccgg aagaacgtga tggtttcctg ccaaaaatca agcggtggcc
     4321 cggaggcggc ggcgttcgtg aaggtttcta aaggagaaga acttttcaca ggagttgttc
     4381 caatacttgt tgaacttgat ggagatgtaa atggacataa gttctctgtt tctggcgaag
     4441 gagaaggaga tgctacttat ggaaaactta ctctcaagtt catttgcact actggaaaac
     4501 ttcccgttcc atggccaact cttgttacta ctttgactta tggagttcaa tgtttcgctc
     4561 gttatccaga ccatatgaaa caacacgatt tcttcaaatc tgctatgcca gaaggatatg
     4621 ttcaagaaag aactattttc ttcaaagatg atggaaacta caaaactcgt gcggaagtaa
     4681 agtttgaggg agatactctt gtaaatagaa tagaactcaa aggaatagat ttcaaagaag
     4741 atggaaatat acttggacat aaacttgaat ataactacaa tagtcataaa gtttatatta
     4801 ctgctgataa acaaaaaaat ggaataaaag taaacttcaa aactcgtcat aatatagaag
     4861 atggaagtgt tcaacttgct gaccattacc aacaaaatac tccaatagga gatggcccag
     4921 ttcttctccc agataatcat tatctttcta ctcaatctgc tctttctaaa gacccaaatg
     4981 aaaaaagaga ccatatggtt cttttagagt tcgttactgc ggcgggaata actcttggaa
     5041 tggatgaact ttacaaatga gagctccgca tcggccgctg tcatcagatc gccatctcgc
     5101 gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc
     5161 ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt
     5221 ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat
     5281 tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc
     5341 aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt
     5401 gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat
     5461 gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat catgctcatc
     5521 gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa
     5581 ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc gaggacggcg
     5641 tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc ggaagaaggt
     5701 ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt ggagtagtgt
     5761 ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac ataactgttt
     5821 actagtgagt cgtattataa gtgcaagtaa gatcagtgtt tgtttcgata tcagtctgtt
     5881 tcgtaacggt cttctgtata actacaaaaa agaataaaaa accgtatcac aactgagaaa
     5941 aaagacaaaa cttattgaac agtgttcctt tcaaaaaatt attttgtaaa aaatcgagtt
     6001 tttctccaaa ataacgcacg caatcaaaaa caagaaaact aacgtgttct cctgggcgtg
     6061 gaactcctta tcaagattac cattattgtc tgtcagaatt gatatttcaa ttatttcata
     6121 tctagacaaa cccgggtgtc ttgcatttat tagtactgtg agtttacata caaacgaggc
     6181 ttactcagat aacatagagt cacctctaat tagcaaaaac ccatttctaa ttagctattt
     6241 cgaccagttc taaagtcacc gtaattagaa atggtttttg tagcttttct aattagaaat
     6301 gaccccgaga taagtgaaat atggcatttt ctgggttttc tagtaagaaa tgatcccgca
     6361 cgtagtgatt ccgcgttaca agccaaattc aaattaaaaa agtatattac acaattcaag
     6421 ttacattatt ttcaggaaac aatggcgaag agcaaagcag agattcaaaa ggaggaaaga
     6481 gacaacagat atgaacgaag acttcttgaa aagagaaaga gacatgaggt gagtaaccat
     6541 gataataaat aatcaagtaa gggctataga tttataaatt ctggagttaa cagtttcgaa
     6601 aaaaagtagt tttcatatgt gcgaaggaat atcaaaaata ggggaatagc ctaaacgagt
     6661 gagaaaatta aatgtttgtt attttttcaa aaatgtctgc agggccgtta tcctttcaaa
     6721 tgcactctat caaacataga tcatatccat catatcttat ccagaattta tatcatatcc
     6781 aagattgaga gcgtttcaag gaaaatctca aacaaccata caattttgta gatcgacaac
     6841 aaggcctcca cttcttcatc gatggcctct gcagcacctg caccaccaag tttgtatttt
     6901 tcatattaat ataaagtgag ccacgcatag tcgtttttag gatttttgcc agatcgatgt
     6961 cgcgcaaaca gactgttttt ttttctttga aagccgtgcg tgacatgggc cacggattgt
     7021 aacgccgcgc tggatggata aaaatttatt cagtttttta gcctttacaa aatatggttc
     7081 gactactttc tttattttta catttttata ggtgctcatt gagctgagat attttgggta
     7141 taatcggaaa atcgctgatt ttggtcattt ttctgcacta aagattacac aaaaagttca
     7201 gtatttagat tcattgttga aaattgggga tgatctacag atacatatcg ccgtgttcaa
     7261 aaacacacgt gctgtgacgt cgtgactggg gaaaaccctg gcgttaccca acttaatcgc
     7321 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7381 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     7441 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtgcaat ctgctctgat
     7501 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7561 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7621 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     7681 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7741 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7801 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7981 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8041 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8161 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8401 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8461 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8581 atcattgcag cactggggtc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8701 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9001 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9481 agcaacgcgg cctttttacg gttcctggtc ttttgctggc cttttgctca catgttcttt
     9541 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9601 agcggataac aatttcaca
//
