LOCUS       sequence-246109-       15003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the inducible expression of S11-GFP1–9 fragment 
            that can be used to detect a protein tagged with GFP10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15003)
  AUTHORS   Liu TY
  TITLE     Using Tripartite Split-sfGFP for the Study of Membrane 
            Protein-Protein Interactions.
  JOURNAL   Methods Mol Biol. 2021;2200:323-336. doi: 
            10.1007/978-1-0716-0880-7_15.
  PUBMED    33175385
REFERENCE   2  (bases 1 to 15003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2021"; volume: "2200:323-336. doi"; pages: " 
            10.1007/978-1-0716-0880-7_15"
COMMENT     Sequence Label: UBQ10:sXVE:S11-GFP1–9
FEATURES             Location/Qualifiers
     source          1..15003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(28..831)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(139..158)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     749..768
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(881..1060)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4322..4368
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      5104..5356
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5523..5547
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6012..6662
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(6850..6867)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7101..7120)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            7343..7452
                     /note="incP origin of transfer"
     CDS             7485..7856
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      7983..8694
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  9317..10084
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             10276..11070
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             11369..12517
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14113..14137
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(14384..14405)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14596..14615
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14740..14762
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14754..14771
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14755..14771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(14784..14984)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
ORIGIN
        1 atccgtcgaa gctagcttgg gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
       61 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
      121 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
      181 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
      241 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
      301 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
      361 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
      421 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
      481 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
      541 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
      601 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
      661 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
      721 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
      781 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc caagctccca tgggccctcg
      841 actagagtcg agatctggat tgagagtgaa tatgagactc taattggata ccgaggggaa
      901 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
      961 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
     1021 agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     1081 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     1141 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     1201 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     1261 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     1321 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     1381 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
     1441 agtgcttgcg gcagcgtgaa gcttcgacga gtcagtaata aacggcgtca aagtggttgc
     1501 agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa
     1561 aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat
     1621 tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt
     1681 ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt
     1741 tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat
     1801 ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt
     1861 atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat
     1921 cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa
     1981 taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta gttctggtgt
     2041 tagtttctag tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacagac
     2101 tagcccgcgg aaaatgaagg ctcttactgc tagacaacaa gaagtttttg atcttattag
     2161 agatcatatt tctcaaactg gaatgcctcc tactagagct gaaattgctc aaagacttgg
     2221 atttaggtct cctaatgctg ctgaagaaca tcttaaggct cttgctagaa agggagttat
     2281 tgaaattgtt tctggagctt caagaggaat tagacttctt caagaagaag aagaaggact
     2341 tcctcttgtt ggaagagttg ctgctggaga accttcttct gctcctccta ctgatgtttc
     2401 tcttggagat gaacttcatc ttgatggaga agatgttgct atggctcatg ctgatgctct
     2461 tgatgatttt gatcttgata tgcttggaga tggagattct cctggacctg gatttactcc
     2521 tcatgattct gctccttatg gagcacttga tatggctgat tttgaatttg aacaaatgtt
     2581 tactgatgct cttggaattg atgaatatgg aggagatcct tctgctggag atatgagagc
     2641 tgctaatctt tggccttctc ctcttatgat taagaggtct aagaagaact ctcttgctct
     2701 ttctcttact gctgaccaaa tggtttctgc tcttcttgat gctgaacctc ctattcttta
     2761 ttctgaatat gatcctacta gacctttttc tgaggcttct atgatgggac ttcttactaa
     2821 tcttgctgat agagaacttg ttcatatgat taattgggct aagagagttc ctggatttgt
     2881 tgatcttact cttcatgacc aagttcatct tcttgaatgt gcttggcttg aaattcttat
     2941 gattggactt gtttggaggt ctatggaaca tcctgttaaa cttctttttg ctcctaatct
     3001 tcttcttgat agaaatcaag gaaagtgtgt tgaaggaatg gttgaaattt ttgatatgct
     3061 tcttgctact tcttcaagat ttagaatgat gaatcttcaa ggagaagaat ttgtttgtct
     3121 taagtctatt attcttctta attctggagt ttatactttt ctttcttcta ctcttaagtc
     3181 tcttgaagaa aaggatcata ttcatagagt tcttgataag attactgata ctcttattca
     3241 tcttatggct aaggctggac ttactcttca acaacaacat caaagacttg ctcaacttct
     3301 tcttattctt tctcatatta gacatatgtc taataaggga atggaacatc tttattctat
     3361 gaagtgtaag aatgttgttc ctctttatga tcttcttctt gaaatgcttg atgctcatag
     3421 acttcatgct cctacttcaa gaggaggagc ttctgttgaa gaaactgacc aatctcatct
     3481 tgctactgct ggatctactt cttctcattc tcttcaaaag tattatatta ctggagaagc
     3541 tgaaggattt cctgctactg tttgaagatc ttgatcaagc tccctggcca attcccagag
     3601 atgttagctg aaatcatcac taatcagata ccaaaatatt caaatggaaa tatcaaatag
     3661 cttctgtttc atcaaaaatg actcgaccta actgagtaag catgcttgtt cgagtattat
     3721 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
     3781 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
     3841 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
     3901 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
     3961 tcgtggcctc taatgaccga agttaatatg aggagtaaaa catcccaaac gcggccgcaa
     4021 ccggtcttgc atccagctgg gctgcaggtc gaggctaaaa aactaatcgc attatcatcc
     4081 cctcgacgta ctgtacatat aaccactggt tttatataca gcagtactgt acatataacc
     4141 actggtttta tatacagcag tcgacgtact gtacatataa ccactggttt tatatacagc
     4201 agtactgtac atataaccac tggttttata tacagcagtc gaggtaagat tagatatgga
     4261 tatgtatatg gatatgtata tggtggtaat gccatgtaat atgctcgact ctaggatctt
     4321 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaagct
     4381 agtcgactct agccaaatct agaatggaaa agcgtgacca catggtcctt cttgagtatg
     4441 taactgctgc tgggattaca gatgctagcg gtggcggttc tggcggtggt actagtatga
     4501 gaaaaggaga agaacttttc actggagttg tcccaattct tattgaatta gatggtgatg
     4561 ttaatgggca caaatttttt gtccgtggag agggtgaagg tgatgcaaca atcggaaaac
     4621 ttagccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
     4681 ctacccttac ctatggtgtt caatgctttt caagataccc agatcatatg aagcggcacg
     4741 acttcttcaa gagcgccatg cctgagggat acgtgcagga gaggaccatc tatttcaagg
     4801 acgacgggac ttacaagaca cgtgctgaag tcaagtttga gggagacacc ctcgtcaaca
     4861 ggatcgagct taagggaatt gatttcaagg aggacggaaa catcctcggc cacaagttgg
     4921 aatacaactt taactcccac aaagtataca tcacggccga caagcaaaat aacggcatca
     4981 aagccaactt cacaattcgc cacaacgttg aagacggctc tgtgcaactc gctgatcatt
     5041 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accataagag ctcgaatttc
     5101 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     5161 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     5221 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     5281 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     5341 tctatgttac tagatcggga attcagatcg gctgagtggc tccttcaacg ttgcggttct
     5401 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     5461 ccttaattct ccgctcatga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg
     5521 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
     5581 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     5641 cagggttccc cagatctggc gccggccagc gagacgagca agattggccg ccgcccgaaa
     5701 cgatccgaca gcgcgcccag cacaggtgcg caggcaaatt gcaccaacgc atacagcgcc
     5761 agcagaatgc catagtgggc ggtgacgtcg ttcgagtgaa ccagatcgcg caggaggccc
     5821 ggcagcaccg gcataatcag gccgatgccg acagcgtcga gcgcgacagt gctcagaatt
     5881 acgatcaggg gtatgttggg tttcacgtct ggcctccgga ccagcctccg ctggtccgat
     5941 tgaacgcgcg gattctttat cactgataag ttggtggaca tattatgttt atcagtgata
     6001 aagtgtcaag catgacaaag ttgcagccga atacagtgat ccgtgccgcc ctggacctgt
     6061 tgaacgaggt cggcgtagac ggtctgacga cacgcaaact ggcggaacgg ttgggggttc
     6121 agcagccggc gctttactgg cacttcagga acaagcgggc gctgctcgac gcactggccg
     6181 aagccatgct ggcggagaat catacgcatt cggtgccgag agccgacgac gactggcgct
     6241 catttctgat cgggaatgcc cgcagcttca ggcaggcgct gctcgcctac cgcgatggcg
     6301 cgcgcatcca tgccggcacg cgaccgggcg caccgcagat ggaaacggcc gacgcgcagc
     6361 ttcgcttcct ctgcgaggcg ggtttttcgg ccggggacgc cgtcaatgcg ctgatgacaa
     6421 tcagctactt cactgttggg gccgtgcttg aggagcaggc cggcgacagc gatgccggcg
     6481 agcgcggcgg caccgttgaa caggctccgc tctcgccgct gttgcgggcc gcgatagacg
     6541 ccttcgacga agccggtccg gacgcagcgt tcgagcaggg actcgcggtg attgtcgatg
     6601 gattggcgaa aaggaggctc gttgtcagga acgttgaagg accgagaaag ggtgacgatt
     6661 gatcaggacc gctgccggag cgcaacccac tcactacagc agagccatgt agacaacatc
     6721 ccctccccct ttccaccgcg tcagacgccc gtagcagccc gctacgggct ttttcatgcc
     6781 ctgccctagc gtccaagcct cacggccgcg ctcggcctct ctggcggcct tctggcgctc
     6841 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6901 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6961 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7021 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7081 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7141 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7201 cgtggcgctt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
     7261 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt
     7321 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt
     7381 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc
     7441 gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa gcggatggct gatgaaacca
     7501 agccaaccag gaagggcagc ccacctatca aggtgtactg ccttccagac gaacgaagag
     7561 cgattgagga aaaggcggcg gcggccggca tgagcctgtc ggcctacctg ctggccgtcg
     7621 gccagggcta caaaatcacg ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca
     7681 tcaatggcga cctgggccgc ctgggcggcc tgctgaaact ctggctcacc gacgacccgc
     7741 gcacggcgcg gttcggtgat gccacgatcc tcgccctgct ggcgaagatc gaagagaagc
     7801 aggacgagct tggcaaggtc atgatgggcg tggtccgccc gagggcagag ccatgacttt
     7861 tttagccgct aaaacggccg gggggtgcgc gtgattgcca agcacgtccc catgcgctcc
     7921 atcaagaaga gcgacttcgc ggagctggtg aagtacatca ccgacgagca aggcaagacc
     7981 gagcgccttt ccgacgctca ccgggctggt tgccctcgcc gctgggctgg cggccgtcta
     8041 tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg
     8101 gcgttgtgga tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga
     8161 cacttgaggg gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg
     8221 gccggcgacg tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag
     8281 tttcccacag atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag
     8341 gggcgcgact actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga
     8401 cagatgaggg gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat
     8461 ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa
     8521 accaatattt ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac
     8581 gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat
     8641 ccccccaggg gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcagcgctgg
     8701 cagtccttgc cattgccggg atcggggcag taacgggatg ggcgatcagc ccgagcgcga
     8761 cgcccggaag cattgacgtg ccgcaggtgc tggcatcgac attcagcgac caggtgccgg
     8821 gcagtgaggg cggcggcctg ggtggcggcc tgcccttcac ttcggccgtc ggggcattca
     8881 cggacttcat ggcggggccg gcaattttta ccttgggcat tcttggcata gtggtcgcgg
     8941 gtgccgtgct cgtgttcggg ggtgcgataa acccagcgaa ccatttgagg tgataggtaa
     9001 gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct atgaagcgcc
     9061 atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag attgccttga
     9121 atatattgac aatactgata agataatata tcttttatat agaagatatc gccgtatgta
     9181 aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata gaatgggcaa
     9241 agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt atagcttgta
     9301 aattctatca taattgggta atgactccaa cttattgata gtgttttatg ttcagataat
     9361 gcccgatgac tttgtcatgc agctccaccg attttgagaa cgacagcgac ttccgtccca
     9421 gccgtgccag gtgctgcctc agattcaggt tatgccgctc aattcgctgc gtatatcgct
     9481 tgctgattac gtgcagcttt cccttcaggc gggattcata cagcggccag ccatccgtca
     9541 tccatatcac cacgtcaaag ggtgacagca ggctcataag acgccccagc gtcgccatag
     9601 tgcgttcacc gaatacgtgc gcaacaaccg tcttccggag actgtcatac gcgtaaaaca
     9661 gccagcgctg gcgcgattta gccccgacat agccccactg ttcgtccatt tccgcgcaga
     9721 cgatgacgtc actgcccggc tgtatgcgcg aggttaccga ctgcggcctg agttttttaa
     9781 gtgacgtaaa atcgtgttga ggccaacgcc cataatgcgg gctgttgccc ggcatccaac
     9841 gccattcatg gccatatcaa tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt
     9901 gaactgcagt tgccatgttt tacggcagtg agagcagaga tagcgctgat gtccggcggt
     9961 gcttttgccg ttacgcacca ccccgtcagt agctgaacag gagggacagc tgatagaaac
    10021 agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat
    10081 cacccataat tgtggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga
    10141 aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga
    10201 attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga
    10261 ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa
    10321 aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg
    10381 ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat
    10441 gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag
    10501 gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc
    10561 gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat
    10621 gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc
    10681 ttagacagcc gcttagccga attggattac ttactgaata acgatctggc cgatgtggat
    10741 tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta
    10801 aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac
    10861 atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg
    10921 gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa
    10981 cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa
    11041 tattatattt tactggatga attgttttag tacctagatg tggcgcaacg atgccggcga
    11101 caagcaggag cgcaccgact tcttccgcat caagtgtttt ggctctcagg ccgaggccca
    11161 cggcaagtat ttgggcaagg ggtcgctggt attcgtgcag ggcaagattc ggaataccaa
    11221 gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta
    11281 tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg
    11341 agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag
    11401 gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac
    11461 cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac
    11521 ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc
    11581 cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga agtcgatgac
    11641 catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc
    11701 aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa
    11761 ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc
    11821 aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc
    11881 gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt
    11941 cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg
    12001 cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg
    12061 gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac
    12121 ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg
    12181 cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga
    12241 aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa
    12301 gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc
    12361 gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg
    12421 gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc
    12481 ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc
    12541 tgggggttca gcagccagcg ctttactggc atttcaggaa caagcgggca ctgctcgacg
    12601 cacttgcttc gctcagtatc gctcgggacg cacggcgcgc tctacgaact gccgataaac
    12661 agaggattaa aattgacaat tgtgattaag gctcagattc gacggcttgg agcggccgac
    12721 gtgcaggatt tccgcgagat ccgattgtcg gccctgaaga aagctccaga gatgttcggg
    12781 tccgtttacg agcacgagga gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat
    12841 gccgtggcat tcggcgccta catcgacggc gagatcattg ggctgtcggt cttcaaacag
    12901 gaggacggcc ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa
    12961 cagcgaggcc gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg tttattgctc
    13021 gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt catcctcggc
    13081 gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg cctgccgggc
    13141 ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct
    13201 ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg aactgcgggc
    13261 gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc
    13321 ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg gcaacctccc
    13381 gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct gctcgttcca
    13441 gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt tctcggcctg
    13501 gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg ggggatctcg
    13561 cgactcgaac ctacagttgt ttccttactg ggctttctca gccccagatc tggggtcgat
    13621 cagccgggga tgcatcaggc cgacagtcgg aacttcgggt ccccgacctg taccattcgg
    13681 tgagcaatgg ataggggagt tgatatcgtc aacgttcact tctaaagaaa tagcgccact
    13741 cagcttcctc agcggcttta tccagcgatt tcctattatg tcggcatagt tctcaagatc
    13801 gacagcctgt cacggttaag cgagaaatga ataagaaggc tgataattcg gatctctgcg
    13861 agggagatga tatttgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
    13921 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
    13981 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
    14041 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
    14101 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
    14161 taacacattg cggacgtttt taatgtactg gggtggtttt tcttttcacc agtgagacgg
    14221 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
    14281 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc
    14341 ttataaatca aaagaatagc ccgagatagg gttgagtgtt gttccagttt ggaacaagag
    14401 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
    14461 tggcccacta cgtgaaccat cacccaaatc aagttttttg gggtcgaggt gccgtaaagc
    14521 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
    14581 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gccattcagg ctgcgcaact
    14641 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    14701 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    14761 cgacggccag tgaattaatt cccatcttga aagaaatata gtttaaatat ttattgataa
    14821 aataacaagt caggtattat agtccaagca aaaacataaa tttattgatg caagtttaaa
    14881 ttcagaaata tttcaataac tgattatatc agctggtaca ttgccgtaga tgaaagactg
    14941 agtgcgatat tatgtgtaat acataaattg atgatatagc tagcttagct catcggggga
    15001 tcg
//
