LOCUS       sequence-24611-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, 
            Lichtman JW
  TITLE     Transgenic strategies for combinatorial expression of fluorescent 
            proteins in the nervous system.
  JOURNAL   Nature. 2007 Nov 1. 450(7166):56-62.
  PUBMED    17972876
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Nov 1. 450(7166):56-62."
COMMENT     Sequence Label: CMV-Brainbow-1.1 M
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(34..67)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(75..108)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(117..150)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      164..173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtttagtga accgtcagat ccgctagctc gagataactt cgtatagtat accttatacg
       61 aagttatcga gctcataact tcgtatagga tactttatac gaagttataa ctcgagataa
      121 cttcgtatag catacattat acgaagttat accggcagat ctcgccacca tggtgagtgt
      181 gattaaacca gagatgaaga tgaagtactt catggacggc tccgtcaatg ggcatgagtt
      241 cacagttgaa ggtgaaggca caggcaaacc ttacgaggga catcaagaga tgacactacg
      301 cgtcacaatg gccaagggcg ggccaatgcc tttctcgttt gacttagtgt cacacacgtt
      361 ctgttacggc cacagacctt ttactaaata tccagaagag ataccagact atttcaaaca
      421 agcatttcct gaaggcctgt catgggaaag gtcgttgcag ttcgaagatg gtgggttcgc
      481 tgccgtcagt gcgcatataa gccttagagg aaactgcttc gagcacaaat ccaaatttgt
      541 tggggttaac tttcctgccg atggtcctgt gatgcaaaac caaagttctg attgggagcc
      601 atcaaccgag aaaattacta cctgcgacgg agttctgaag ggtgatgtta cgatgtacct
      661 aaaacttgcg ggaggcggca atcacaaatg ccaattcaag actacttaca aggcggcaaa
      721 aaa
//
