LOCUS       sequence-246126-        7352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 1 vector of PlaMiNGo toolkit for expression of plastid 
            targeted GFP1-10 in plant cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB
  TITLE     Targeting specificity of nuclear-encoded organelle proteins with a 
            self-assembling split-fluorescent protein toolkit.
  JOURNAL   J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 
            10.1242/jcs.230839.
  PUBMED    31085714
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-06-3"; volume: "132(11). pii: jcs.230839. doi"; 
            pages: " 10.1242/jcs.230839"
COMMENT     Sequence Label: pTEI126
FEATURES             Location/Qualifiers
     source          1..7352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             605..1246
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     terminator      1277..1984
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    2033..2057
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(2213..3361)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(3697..4285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3777..3796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4456..5247,5248..5316))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5079..5098
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5317..5421)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5508..6139
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    6231..6255
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt
      241 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag
      361 agctcatttt tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt
      421 tacaattatc gataccatga ccaccgctgt caccgccgct gtttctttcc cctctaccaa
      481 aaccacctct ctctccgccc gaagctcctc cgtcatttcc cctgacaaaa tcagctacaa
      541 aaaggttcct ttgtactaca ggaatgtatc tgcaactggg aaaatgggac ccatcagggc
      601 cgcaatgtcc aaaggagaag aactgtttac cggtgttgtg ccaattttgg ttgaactcga
      661 tggtgatgtc aacggacata agttctcagt gagaggcgaa ggagaaggtg acgccaccat
      721 tggaaaattg actcttaaat tcatctgtac tactggtaaa cttcctgtac catggccgac
      781 tctcgtaaca acgcttacgt acggagttca gtgcttttcg agatacccag accatatgaa
      841 aagacatgac ttttttaagt cggctatgcc tgaaggttac gtgcaagaaa gaacaatttc
      901 gttcaaagat gatggaaaat ataaaactag agcagttgtt aaatttgaag gagatacttt
      961 ggttaaccgc attgaactga aaggaacaga ttttaaagaa gatggtaata ttcttggaca
     1021 caaactcgaa tacaatttta atagtcataa cgtatacatc actgctgata agcaaaagaa
     1081 cggaattaaa gcgaatttca cagtacgcca taatgtagaa gatggcagtg ttcaacttgc
     1141 cgaccattac caacaaaaca cccctattgg agacggtccg gtacttcttc ctgataatca
     1201 ctacctctca acacaaacag tcctgagcaa agatccaaat gaaaaaggaa caggtggcgg
     1261 cggaagttag cttgtcctgc tttaatgaga tatgcgagaa gcctatgatc gcatgatatt
     1321 tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta
     1381 ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat
     1441 aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg
     1501 aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa
     1561 caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa
     1621 acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca ggggctagta
     1681 tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc ggttccccgc
     1741 cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt
     1801 acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga
     1861 attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac
     1921 agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca
     1981 gcaggaccgc tactattgtc ttctgcacga agtggtttaa actatcagtg tttgacagga
     2041 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
     2101 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
     2161 cagatcaggc gctggctgct gaacccccag ccggaactga ccccacaagg ccctagcgtt
     2221 tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg cctcgcaact
     2281 cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc
     2341 acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt
     2401 cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg aatttcgtgt
     2461 agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg caacgggacg
     2521 ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc
     2581 caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg
     2641 ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg
     2701 tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc tgctgccaca
     2761 ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc acgtccttgt
     2821 tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg
     2881 tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg
     2941 caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg
     3001 cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt tttcgcttct
     3061 tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct
     3121 gttcaagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca gggggagcca
     3181 gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag ccgacggact
     3241 ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg
     3301 cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac gtccgattca
     3361 ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct tattcctgat
     3421 ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg aagtcggtcc
     3481 cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc tgcacgaata
     3541 ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc ctgagagcca aaacacttga
     3601 tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac atctaggatc
     3661 tgccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4561 cttaccatct ggccccagtg ctgcaatgat accgcgagaa ccacgctcac cggctccaga
     4621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5341 aagcatttat caaggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5401 taaacaaata ggggttccgc gcacgaattg gccagcgctg ccatttttgg ggtgaggccg
     5461 ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag
     5521 ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc
     5581 ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg
     5641 gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc
     5701 ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga
     5761 tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat
     5821 ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat
     5881 ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa
     5941 cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag
     6001 ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt
     6061 cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc
     6121 aaccagcccg gtgagcgtcg caaaggagat cctgatctga ctgatgggct gcctgtatcg
     6181 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
     6241 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
     6301 atgtactggg gtggatgcag tgggccccac tctgtgaaga caagcaagaa ttcaagcttg
     6361 gaggaattcc aatcccacaa aaatctgagc ttaacagcac agttgctcct ctcagagcag
     6421 aatcgggtat tcaacaccct catatcaact actacgttgt gtataacggt ccacatgccg
     6481 gtatatacga tgactggggt tgtacaaagg cggcaacaaa cggcgttccc ggagttgcac
     6541 acaagaaatt tgccactatt acagaggcaa gagcagcagc tgacgcgtac acaacaagtc
     6601 agcaaacaga caggttgaac ttcatcccca aaggagaagc tcaactcaag cccaagagct
     6661 ttgctaaggc cctaacaagc ccaccaaagc aaaaagccca ctggctcacg ctaggaacca
     6721 aaaggcccag cagtgatcca gccccaaaag agatctcctt tgccccggag attacaatgg
     6781 acgatttcct ctatctttac gatctaggaa ggaagttcga aggtgaaggt gacgacacta
     6841 tgttcaccac tgataatgag aaggttagcc tcttcaattt cagaaagaat gctgacccac
     6901 agatggttag agaggcctac gcagcaagtc tcatcaagac gatctacccg agtaacaatc
     6961 tccaggagat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta
     7021 attgcatcaa gaacacagag aaagacatat ttctcaagat cagaagtact attccagtat
     7081 ggacgattca aggcttgctt cataaaccaa ggcaagtaat agagattgga gtctctaaaa
     7141 aggtagttcc tactgaatct aaggccatgc atggagtcta agattcaaat cgaggatcta
     7201 acagaactcg ccgtcaagac tggcgaacag ttcatacaga gtcttttacg actcaatgac
     7261 aagaagaaaa tcttcgtcaa catggtggag cacgacactc tggtctactc caaaaatgtc
     7321 aaagatacag tctcagaaga tcaaagggct at
//
