LOCUS       sequence-246138-        9857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pben-int5; Ptet-bxb1; Pbad-tp901:  colE1 Chlo+.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Zuniga A, Guiziou S, Mayonove P, Meriem ZB, Camacho M, Moreau V, 
            Ciandrini L, Hersen P, Bonnet J
  TITLE     Rational programming of history-dependent logic in cellular 
            populations.
  JOURNAL   Nat Commun. 2020 Sep 21;11(1):4758. doi: 10.1038/s41467-020-18455-z.
  PUBMED    32958811
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-21"; volume: "11(1):4758. doi"; pages: " 
            10.1038/s41467-020-18455-z"
COMMENT     Sequence Label: Integrase triple controller
FEATURES             Location/Qualifiers
     source          1..9857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1069..1103)
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             3165..3176
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    3248..3266
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3273..3291
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3336..4835
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="RALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDV
                     SGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVV
                     SATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYL
                     PTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGR
                     EPQGREWSATALKRSMISGAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAEL
                     VKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVA
                     MAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGS
                     PQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMN
                     VRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      4864..4891
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(4974..5852)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWAYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5879..6163
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     6086..6105
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             6217..6234
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7702..7734
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag
                     /note="wild-type LAA variant"
                     /translation="AAKDENYALAA"
     misc_feature    7738..7758
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      7759..7816
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(7840..7857)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8067..8654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8147..8166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(8742..8836)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(8857..9516)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTIQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     9431..9450
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(9517..9619)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(9796..9840)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(9843..9857,1..7)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tctagagtat tcggagtaaa acgagttgta atccctctgg ttgaaacggg agaccagaaa
       61 caaaaaaaca ccccgaaggg tgttcaataa ttggttaagc acgacgtttg aaggtctgag
      121 acggcagttc accgaactgc tgacggtaga tttcagagaa acgacccagg tgcaggaaac
      181 cgtagtccag agccagttcg gtaacagaac gaacaccgca ggtcgggtca gacaggcaag
      241 cgtgaacacg ttccagttta cgctgacgaa cgtagtgttt cggggtgata cccaggtgct
      301 ggtcgaacag agcgtacaga gaacgcagag acatgcaagc ctgttcagcc agaacttcag
      361 cagacagttc cagtttcagg ttacgttcga tgtagtccag gatacgttcc agaccagcct
      421 gcggagcaga cagagattca cgacggatgt tggtagacat cagggtcagc agtttagaag
      481 caacgatctg agagtagtga ccctgaacac gcggcagaga gtcagaaact tcagcttcgt
      541 ggcaaaccat agccagcagg ttaacgaaac cgtccagttc gtccagacgg tagtggttac
      601 gcaggaaacg aacaccaccg tccggacgct gccaacgctg ttcgtcgcag atagagtcca
      661 gcagacgggt cggaactttc aggatgaatt tttcgcagtc ttcagagtag gtcaggtcaa
      721 ccgggtcgtc cgggttgatc agcagcagtt cacccggaac caggtgctgt tcacgtttgt
      781 gaccacgcca caggcagtta ccgttcagca gaacctgcag gtggtagatg gtttccagag
      841 ccggagaggt aacacgaaca gaaccaccgt aagagatacg gcacaggtcc agttcagcga
      901 atttacggtg agacagagaa gcctgcgggt gggtggtacg agacagaccg atgcagtgct
      961 gaccaacgtg ctggttaacg tagtcagaaa cagcgtacgg gtcagcgtgg tggaaaacag
     1021 aagaacgttc agacagcaga cgagattcca tctagtagtc ctgtgtgagc tagcactata
     1081 cctaggactg agctagccgt aaatcttcta cgataaatac ttactgcgtg acgagggaaa
     1141 tctttttgtt atcaataaaa aaggcccccc gttagggagg ccttattgtt cgtcgttgag
     1201 taagataaac cacccttctg tagtagaccg tattgatact gttacttacc gattattgtg
     1261 aagaaccaga ccgtactgtt cgaagcattg ccattttctg aagttaccga aaaagtaccg
     1321 aacatccgta aatctggata acgttctgca caatccggat agccccccgc cagccgtctc
     1381 cctaacctga ccagaagctt tcacacagga ctactagatg cctggtatga ccaccgaaac
     1441 cggtccggat ccggcaggtc tgattgacct gttttgtcgt aaaagcaaag cagttaaaag
     1501 ccgtgcaaat ggtgcaggtc agcgtcgtaa acaagaaatt agcattgcag cacaagaaac
     1561 cctgggtcgt aaagttgcag cactgctggg tatgcaggtt cgtcatgttt ggaaagaagt
     1621 tggtagcgca agccgttttc gtaaaggtaa agcacgtgat gatcagagca aagcactgaa
     1681 agccctggaa agcggtgaag ttggtgcact gtggtgttat cgtctggatc gttgggatcg
     1741 tggtggtgcc ggtgcaattc tgaaaattat cgaaccggaa gatggtatgc ctcgtcgtct
     1801 gctgtttggt tgggatgaag ataccggtcg tccggttctg gatagcacca ataaacgtga
     1861 tcgcggtgaa ctgattcgtc gtgcagaaga agcacgcgaa gaagcagaaa aactgagcga
     1921 acgtgttcgt gataccaaag cacatcagcg tgaaaatggt gaatgggtta atgcccgtgc
     1981 accgtatggt ctgcgtgttg ttctggttac cgttagtgat gaagagggtg atgaatatga
     2041 tgaacgtaaa ctggcagcag atgatgaaga tgcgggtggt cctgatggtc tgaccaaagc
     2101 agaagcagcc cgtctggttt ttaccctgcc ggttaccgat cgtctgagct atgcaggcac
     2161 cgcacatgca atgaataccc gtgaaattcc gagcccgacc ggtggtccgt ggattgcagt
     2221 taccgtgcgt gatatgattc agaatccggc atatgcgggt tggcagacca caggtcgtca
     2281 ggatggtaaa cagcgtcgtc tgacctttta taacggtgaa ggtaaacgtg ttagcgttat
     2341 gcatggtcct ccgctggtga ccgatgaaga acaagaagcc gcaaaagcag ccgttaaagg
     2401 tgaagatggt gttggtgttc cgctggatgg tagcgatcat gatacccgtc gcaaacatct
     2461 gctgagcggt cgtatgcgtt gtccgggttg tggtggtagc tgtagctata gcggtaatgg
     2521 ttatcgttgt tggcgtagca gtgtgaaagg tggttgtccg gcaccgacct atgttgcacg
     2581 taaaagcgtt gaagaatatg ttgcatttcg ttgggcagca aaattagcag caagcgaacc
     2641 ggatgatccg tttgttattg cagttgcaga tcgctgggca gcactgaccc atccgcaggc
     2701 aagcgaagat gaaaagtatg caaaagccgc agttcgtgaa gccgaaaaaa atctgggtcg
     2761 cctgctgcgt gatcgtcaga atggtgttta tgatggtccg gcagaacagt tttttgcccc
     2821 tgcatatcaa gaagcactga gcaccttgca ggcagccaaa gatgcagtta gcgaaagcag
     2881 cgcaagcgca gcagttgatg ttagctggat tgttgatagc agcgattatg aagaactgtg
     2941 gctgcgtgca accccgacca tgcgtaatgc aattattgat acctgcatcg atgaaatttg
     3001 ggttgcaaaa ggccagcgtg gtcgtccgtt tgatggtgat gaacgcgtta aaatcaaatg
     3061 ggcagcccgt accgcagcaa acgacgaaaa ctacgctgca tcagtttaat aaactagtaa
     3121 cgattacgga ttgctctact gtgactgaag tttacaaccc aattattgaa ggccgctaac
     3181 gcggcctttt tttgtttctg gtctccctga ggcacagaga tttactttat tcacgacttc
     3241 agatacgtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca
     3301 caagcttgcg gctttcacac ataggagcta gcgtgagagc cctggtagtc atccgcctgt
     3361 cccgcgtcac cgatgctacg acttcaccgg agcgtcagct ggagtcttgc cagcagctct
     3421 gcgcccagcg cggctgggac gtcgtcgggg tagcggagga tctggacgtc tccggggcgg
     3481 tcgatccgtt cgaccggaag cgcagaccga acctggcccg gtggctagcg ttcgaggagc
     3541 aaccgtttga cgtgatcgtg gcgtaccggg tagatcggtt gacccgatcg atccggcatc
     3601 ttcagcagct ggtccactgg gccgaggacc acaagaagct ggtcgtctcc gcgaccgaag
     3661 cgcacttcga tacgacgacg ccgtttgcgg cggtcgtcat cgcgcttatg ggaacggtgg
     3721 cgcagatgga attagaagcg atcaaagagc ggaaccgttc ggctgcgcat ttcaatatcc
     3781 gcgccgggaa ataccgagga tccctgccgc cgtggggata cctgcctacg cgcgtggacg
     3841 gggagtggcg gctggtgccg gaccctgtgc agcgagagcg catcctcgag gtgtatcacc
     3901 gcgtcgtcga caaccacgag ccgctgcatc tggtggccca cgacctgaac cggcgtggtg
     3961 tcctgtcgcc gaaggactac ttcgcgcagc tgcaaggccg cgagccgcag ggccgggagt
     4021 ggtcggctac cgcgctgaag cgatcgatga tctccggggc gatgctcggg tacgcgactc
     4081 tgaacggtaa gaccgtccga gacgacgacg gagccccgct ggtgcgggct gagccgatcc
     4141 tgacccgtga gcagctggag gcgctgcgcg ccgagctcgt gaagacctcc cgggcgaagc
     4201 ccgcggtgtc taccccgtcg ctgctgctgc gggtgttgtt ctgcgcggtg tgcggggagc
     4261 ccgcgtacaa gttcgccggg ggaggacgta agcacccgcg ctaccgctgc cgctcgatgg
     4321 ggttcccgaa gcactgcggg aacggcacgg tggcgatggc cgagtgggac gcgttctgcg
     4381 aggagcaggt actggatctg ctcggggacg cggagcgtct ggagaaagtc tgggtagcgg
     4441 gctcggactc cgcggtcgaa ctcgcggagg tgaacgcgga gctggtggac ctgacgtcgc
     4501 tgatcggctc cccggcctac cgggcgggct ctccgcagcg agaagcactg gatgcccgta
     4561 ttgcggcgct ggccgcgcgg caagaggagc tggagggcct ggaggctcgc ccgtctggct
     4621 gggagtggcg cgagaccggg cagcggttcg gggactggtg gcgggagcag gacaccgcgg
     4681 caaagaacac ctggcttcgg tcgatgaacg ttcggctgac gttcgacgtc cgcggcgggc
     4741 tgactcgcac gatcgacttc ggggatcttc aggagtacga gcagcatctc aggctcggca
     4801 gcgtggtcga acggctacac accgggatgt cgtaataata ctagatgcat taataatcac
     4861 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag
     4921 ccagattatt aatccggctt ttttattatt tcctgcaggg cggccgccat atgttatgac
     4981 aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg
     5041 gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg
     5101 cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata
     5161 cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac
     5221 agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct
     5281 gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg
     5341 agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc
     5401 agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg
     5461 ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac
     5521 ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaagccca ctggtgatac
     5581 cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa
     5641 atatcacccg gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga
     5701 atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga
     5761 taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc
     5821 ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa
     5881 gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc
     5941 tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa
     6001 gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat
     6061 tatttgcacg gcgtcactct ttgctatgcc atagcatttt tatccataag attagcggat
     6121 cctacctgac gctttttatc gcaactctct actgtttctc cataccgttt ttttgggcta
     6181 gctactagag tcacacagga aacctactag atgaaacatc atcaccatca ccaccaggcc
     6241 ggcactaaga aagtagcaat ctatacacga gtatccacta ctaaccaagc agaggaaggc
     6301 ttctcaattg atgagcaaat tgaccgttta acaaaatatg ctgaagcaat ggggtggcaa
     6361 gtatctgata cttatactga tgctggtttt tcaggggcca aacttgaacg cccagcaatg
     6421 caaagattaa tcaacgatat cgagaataaa gcttttgata cagttcttgt atataagcta
     6481 gaccgccttt cacgtagtgt aagagatact ctttatcttg ttaaggatgt gttcacaaaa
     6541 aataaaatag actttatctc gcttaatgaa agtattgata cttcttctgc tatgggtagc
     6601 ttgtttctca ctattctttc tgcaattaat gagtttgaaa gagagaatat aaaagaacgc
     6661 atgactatgg gtaaactagg gcgagcgaaa tctggtaagt ctatgatgtg gactaagaca
     6721 gcttttgggt attaccacaa cagaaagaca ggtatattag aaattgttcc tttacaagct
     6781 acaatagttg aacaaatatt cactgattat ttatcaggaa tatcacttac aaaattaaga
     6841 gataaactca atgaatctgg acacatcggt aaagatatac cgtggtctta tcgtacccta
     6901 agacaaacac ttgataatcc agtttactgt ggttatatca aatttaagga cagcctattt
     6961 gaaggtatgc acaaaccaat tatcccttat gagacttatt taaaagttca aaaagagcta
     7021 gaagaaagac aacagcagac ttatgaaaga aataacaacc ctagaccttt ccaagctaaa
     7081 tatatgctgt cagggatggc aaggtgcggt tactgtggag cacctttaaa aattgttctt
     7141 ggccacaaaa gaaaagatgg aagccgcact atgaaatatc actgtgcaaa tagatttcct
     7201 cgaaaaacaa aaggaattac agtatataat gacaataaaa agtgtgattc aggaacttat
     7261 gatttaagta atttagaaaa tactgttatt gacaacctga ttggatttca agaaaataat
     7321 gactccttat tgaaaattat caatggcaac aaccaaccta ttcttgatac ttcgtcattt
     7381 aaaaagcaaa tttcacagat cgataaaaaa atacaaaaga actctgattt gtacctaaat
     7441 gattttatca ctatggatga gttgaaagat cgtactgatt cccttcaggc tgagaaaaag
     7501 ctgcttaaag ctaagattag cgaaaataaa tttaatgact ctactgatgt ttttgagtta
     7561 gttaaaactc agttgggctc aattccgatt aatgaactat catatgataa taaaaagaaa
     7621 atcgtcaaca accttgtatc aaaggttgat gttactgctg ataatgtaga tatcatattt
     7681 aaattccaac tcgctaccgg tgctgctaag gacgaaaact acgctctggc tgcttaatac
     7741 tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     7801 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     7861 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tcctagctag
     7921 ggcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7981 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8041 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8101 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8161 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8221 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8281 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgta tgcacgaacc ccccgttcag
     8341 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8401 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8461 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8521 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8581 aaacaaacca ccgctggtag cggtggtttt ttgtttgcaa gcagcagatt acgcgcagaa
     8641 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8701 aaaactcacg ttaagggatt ttggtcatga cttgtgcttg gattctcacc aataaaaaac
     8761 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     8821 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     8881 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     8941 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     9001 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     9061 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     9121 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     9181 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     9241 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     9301 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     9361 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaatgg tctggttata
     9421 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     9481 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     9541 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     9601 acctcttacg tgccgatcaa cgtctcattt tcgccagata tcgacgtcta agaaaccatt
     9661 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcacctc
     9721 gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     9781 gtatcacgag gcagaatttc agataaaaaa aaatccttag ctttcgctaa ggatgatttc
     9841 tggaattcgc ggccgct
//
