LOCUS       sequence-24614-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, 
            Lichtman JW
  TITLE     Transgenic strategies for combinatorial expression of fluorescent 
            proteins in the nervous system.
  JOURNAL   Nature. 2007 Nov 1. 450(7166):56-62.
  PUBMED    17972876
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Nov 1. 450(7166):56-62."
COMMENT     Sequence Label: Thy1-Brainbow-1.0 L
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(176..209)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(218..251)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      264..273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(403..421)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     671..690
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgctctgaaa gggataagag aggggctgag gtattcatca tgtgctccgt ggatctcaag
       61 ccctcaaggt aaatggggac ccacctgtcc taccagctgg ctgacctgta gctttcccca
      121 ccacagaatc caagtcggaa ctcttggcac ctagaggatc tcgactagcg agctcataac
      181 ttcgtatagg atactttata cgaagttata actcgagata acttcgtata gcatacatta
      241 tacgaagtta taccggcaga tctgccacca tggtgagcaa gggcgaggag gtcatcaaag
      301 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
      361 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      421 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
      481 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
      541 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
      601 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
      661 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
      721 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggcctgaagc tgaaggaccg
      781 ggggccctac ctggngtgat taanaacntt antggccaaa anccnnngac tgcc
//
