LOCUS       sequence-24615-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, 
            Lichtman JW
  TITLE     Transgenic strategies for combinatorial expression of fluorescent 
            proteins in the nervous system.
  JOURNAL   Nature. 2007 Nov 1. 450(7166):56-62.
  PUBMED    17972876
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Nov 1. 450(7166):56-62."
COMMENT     Sequence Label: Thy1-Brainbow-1.1 M
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(176..209)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(217..250)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(259..292)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      306..315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     gap             670..680
                     /estimated_length=11
ORIGIN
        1 cgctcctaag angggataag agaggggctg aggtattcat catgtgctcc gtggatctca
       61 agccctcaag gtaaatgggg acccacctgt cctaccagct ggctgacctg tagctttccc
      121 caccacagaa tccaagtcgg aactcttggc acctagagga tctcgctagc tcgagataac
      181 ttcgtatagt ataccttata cgaagttatc gagctcataa cttcgtatag gatactttat
      241 acgaagttat aactcgagat aacttcgtat agcatacatt atacgaagtt ataccggcag
      301 atctcgccac catggtgagt gtgattaaac cagagatgaa gatgaagtac ttcatggacg
      361 gctccgtcaa tgggcatgag ttcacagttg aaggtgaagg cacaggcaaa ccttacgagg
      421 gacatcaaga gatgacacta cgcgtcacaa tggccaaggg cgggccaatg cctttctcgt
      481 ttgacttagt gtcacacacg ttctgttacg gccacagacc ttttactaaa tatccagaag
      541 agataccaga ctatttcaaa caagcatttc ctgaaggcct gtcatgggaa aggtcgttgc
      601 agttcgaaga tggtgggttc gctgccgtca gtgcgcatat aagccttaga gangtcacac
      661 nttgcggatn nnnnnnnnnn gnggggnnng tntnngtngg ggccgggtgg naaccnantt
      721 tttggggccn ccccgaaaaa ntncccnccc ngcncgagtg tgaggtgagt acangnctaa
      781 actgcggagc gcatccatgc atcagatntn nagcgcaaan atctaat
//
