LOCUS       sequence-246158-        6066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the red genetically encoded dopamine sensor RdLight1 in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Patriarchi T, Mohebi A, Sun J, Marley A, Liang R, Dong C, Puhger K, 
            Mizuno GO, Davis CM, Wiltgen B, von Zastrow M, Berke JD, Tian L
  TITLE     An expanded palette of dopamine sensors for multiplex imaging in 
            vivo.
  JOURNAL   Nat Methods. 2020 Nov;17(11):1147-1155. doi: 
            10.1038/s41592-020-0936-3. Epub 2020 Sep 7.
  PUBMED    32895537
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0936-3"; journalName: "Nat Methods"; date: 
            "2020-11"; volume: "17"; issue: "11"; pages: "1147-1155"
COMMENT     Sequence Label: pCMV-RdLight1
FEATURES             Location/Qualifiers
     source          1..6066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2490..2611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2527..2546)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2581..2600
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2618..3073)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2755..2776)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2967..2986
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3100..3204
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3206..3563
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3206..3397
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3229..3249)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3414..3549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3598..4392
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3598..4392
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3652..3671)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4262..4281
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4580..4599)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4624..4671
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5000..5588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5489..5508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5763..6066
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttaccggtg cgcgccatga agacgatcta ctagaagctt
      301 accggtgcgc gccatgaaga cgatcatcgc cctgagctac atcttctgcc tggtgttcgc
      361 cgactacaag gacgatgatg acgccatgag gactctgaac acctctgcca tggacgggac
      421 tgggctggtg gtggagaggg acttctctgt tcgtatcctc actgcctgtt tcctgtcgct
      481 gctcatcctg tccacgctcc tggggaacac gctggtctgt gctgccgtta tcaggttccg
      541 acacctgcgg tccaaggtga ccaacttctt tgtcatctcc ttggctgtgt cagatctctt
      601 ggtggccgtc ctggtcatgc cctggaaggc agtggctgag attgctggct tctggccctt
      661 tgggtccttc tgtaacatct gggtggcctt tgacatcatg tgctccactg catccatcct
      721 caacctctgt gtgatcagcg tggacaggta ttgggctatc tccagccctg aacggtatga
      781 gagaaagatg acccccaagg cagccttcat cctgatcagt gtggcatgga ccttgtctgt
      841 actcatctcc ttcatcccag tgcagctcag ctggcacaag gcaaaaccca caagcccctc
      901 tgatggaaat gccacttccc tggctgagac catagacaac tgtgactcca gcctcagcag
      961 gacatatgcc atctcatcct ctgtaatcag cttttacatc cctgtggcca tcatgattgt
     1021 cacctacacc aggatctaca ggattgctca gaaacagctc tcctcctttc atgtgtcaga
     1081 gcggatgtat ccagaagatg gagcgttgaa gtcagagatc aaaaaaggac tgcggttgaa
     1141 ggatggcgga cattatgcag ctgaggtcaa gactacatat aaagctaaaa aacccgttca
     1201 gcttccgggt gcgtatatcg tagatattaa actcgacatt gtttctcaca acgaggatta
     1261 tacaatcgtc gagcaatgcg aaagggcaga aggacgacac tccaccgggg gaatggatga
     1321 gctttataaa ggaggtactg gtggcagcct tgtctcaaag ggggaggaag acaatatggc
     1381 aataattaaa gagttcatga gattcaaagt acacatggag ggaagcgtca atggtcacga
     1441 gttcgagatc gaaggggaag gggagggacg cccttatgag gcctttcaga ccgccaaatt
     1501 gaaagtaaca aagggtggac cccttccatt tgcgtgggac atcctctccc ctcagtttat
     1561 gtatggtagt aaggcttata taaagcatcc ggctgatata ccagattact ttaaactttc
     1621 cttcccggaa gggtttcgct gggaacgcgt catgaatttc gaggatgggg gcattataca
     1681 cgtcaatcaa gatagctcct tgcaagatgg tgtctttatc tataaggtaa agctgagagg
     1741 gacaaatttt ccaccggatg gaccagtaat gcagaagaaa actatggggt gggaagcacg
     1801 gaaggacgac cttaaaagag aaactaaagt cctgaagact ctgtcggtga tcatgggtgt
     1861 gtttgtgtgc tgttggctac ctttcttcat cttgaactgc attttgccct tctgtgggtc
     1921 tggggagacg cagcccttct gcattgattc caacaccttt gacgtgtttg tgtggtttgg
     1981 gtgggctaat tcatccttga accccatcat ttatgccttt aatgctgatt ttcggaaggc
     2041 attttcaacc ctcttaggat gctacagact ttgccctgcg acgaataatg ccatagagac
     2101 ggtgagtatc aataacaatg gggccgcgat gttttccagc catcatgagc cacgaggctc
     2161 catctccaag gagtgcaatc tggtttacct gatcccacat gctgtgggct cctctgagga
     2221 cctgaaaaag gaggaggcag ctggcatcgc cagacccttg gagaagctgt ccccagccct
     2281 atcggtcata ttggactatg acactgacgt ctctctggag aagatccaac ccatcacaca
     2341 aaacggtcag cacccaacct gatgtacagc ggccgcgact ctagatcata atcagccata
     2401 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     2461 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     2521 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2581 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg
     2641 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     2701 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     2761 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     2821 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     2881 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     2941 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     3001 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3061 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3121 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3181 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     3241 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3301 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3361 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3421 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3481 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3541 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     3601 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     3661 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     3721 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     3781 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     3841 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     3901 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     3961 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4021 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4081 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     4141 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     4201 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     4261 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     4321 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     4381 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     4441 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     4501 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     4561 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     4621 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
     4681 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     4741 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     4801 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
     4861 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4921 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4981 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5041 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5101 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5161 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5221 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5281 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5341 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5401 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5461 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5521 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5581 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5641 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
     5701 attagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     5761 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     5821 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     5881 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     5941 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     6001 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     6061 accatg
//
