LOCUS       sequence-246162-        6025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the yellow genetically encoded dopamine sensor YdLight1 in
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Patriarchi T, Mohebi A, Sun J, Marley A, Liang R, Dong C, Puhger K, 
            Mizuno GO, Davis CM, Wiltgen B, von Zastrow M, Berke JD, Tian L
  TITLE     An expanded palette of dopamine sensors for multiplex imaging in 
            vivo.
  JOURNAL   Nat Methods. 2020 Nov;17(11):1147-1155. doi: 
            10.1038/s41592-020-0936-3. Epub 2020 Sep 7.
  PUBMED    32895537
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0936-3"; journalName: "Nat Methods"; date: 
            "2020-11"; volume: "17"; issue: "11"; pages: "1147-1155"
COMMENT     Sequence Label: pCMV-YdLight1
FEATURES             Location/Qualifiers
     source          1..6025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1054..1305
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1242..1263
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1369..1390)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1630..1649)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2450..2571
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2487..2506)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2541..2560
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2578..3032)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2715..2736)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2926..2945
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3059..3163
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3165..3522
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3165..3356
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3188..3208)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3373..3508
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3557..4351
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3557..4351
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3611..3630)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4221..4240
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4539..4558)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4583..4630
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4959..5547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5448..5467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5722..6025
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagt ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttaccggtg cgcgccatga agacgatcat cgccctgagc
      301 tacatcttct gcctggtgtt cgccgactac aaggacgatg atgacgccat gaggactctg
      361 aacacctctg ccatggacgg gactgggctg gtggtggaga gggacttctc tgttcgtatc
      421 ctcactgcct gtttcctgtc gctgctcatc ctgtccacgc tcctggggaa cacgctggtc
      481 tgtgctgccg ttatcaggtt ccgacacctg cggtccaagg tgaccaactt ctttgtcatc
      541 tccttggctg tgtcagatct cttggtggcc gtcctggtca tgccctggaa ggcagtggct
      601 gagattgctg gcttctggcc ctttgggtcc ttctgtaaca tctgggtggc ctttgacatc
      661 atgtgctcca ctgcatccat cctcaacctc tgtgtgatca gcgtggacag gtattgggct
      721 atctccagcc ctgcccggta tgagagaaag atgaccccca aggcagcctt catcctgatc
      781 agtgtggcat ggaccttgtc tgtactcatc tccttcatcc cagtgcagct cagctggcac
      841 aaggcaaaac ccacaagccc ctctgatgga aatgccactt ccctggctga gaccatagac
      901 aactgtgact ccagcctcag caggacatat gccatctcat cctctgtaat cagcttttac
      961 atccctgtgg ccatcatgat tgtcacctac accaggatct acaggattgc tcagaaacag
     1021 ctgagctcac tcattaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg
     1081 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag
     1141 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctat
     1201 cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1261 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg
     1321 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1381 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc
     1441 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1501 accctcgtga ccaccctgac ctacggcgtg cagtgcttcg ctcgctaccc cgaccacatg
     1561 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc
     1621 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1681 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1741 cacaagctgg agtacaacaa tcatgaccaa ctgaaaagag aaactaaagt cctgaagact
     1801 ctgtcggtga tcatgggtgt gtttgtgtgc tgttggctac ctttcttcat cttgaactgc
     1861 attttgccct tctgtgggtc tggggagacg cagcccttct gcattgattc caacaccttt
     1921 gacgtgtttg tgtggtttgg gtgggctaat tcatccttga accccatcat ttatgccttt
     1981 aatgctgatt ttcggaaggc attttcaacc ctcttaggat gctacagact ttgccctgcg
     2041 acgaataatg ccatagagac ggtgagtatc aataacaatg gggccgcgat gttttccagc
     2101 catcatgagc cacgaggctc catctccaag gagtgcaatc tggtttacct gatcccacat
     2161 gctgtgggct cctctgagga cctgaaaaag gaggaggcag ctggcatcgc cagacccttg
     2221 gagaagctgt ccccagccct atcggtcata ttggactatg acactgacgt ctctctggag
     2281 aagatccaac ccatcacaca aaacggtcag cacccaacct gatgtacagc ggccgcgact
     2341 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     2401 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2461 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2521 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     2581 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     2641 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     2701 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     2761 tcaaagggcg aaaaaccgtc tatcagggcg atggccacta cgtgaaccat caccctaatc
     2821 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     2881 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     2941 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3001 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3061 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3121 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     3181 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3241 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3301 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3361 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3421 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3481 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     3541 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3601 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3661 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     3721 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     3781 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     3841 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     3901 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     3961 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4021 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4081 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4141 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4201 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4261 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4321 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4381 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4441 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4501 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     4561 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     4621 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4681 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     4741 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     4801 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4861 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4921 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4981 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5041 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5101 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5161 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5221 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5281 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5341 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5401 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5461 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5521 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5581 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5641 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
     5701 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     5761 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     5821 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     5881 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     5941 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     6001 cgtattagtc atcgctatta ccatg
//
