LOCUS       sequence-246169-       10900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Azizoglu A, Brent R, Rudolf F
  TITLE     A precisely adjustable, variation-suppressed eukaryotic 
            transcriptional controller to enable genetic discovery.
  JOURNAL   Elife. 2021 Aug 3;10. pii: 69549. doi: 10.7554/eLife.69549.
  PUBMED    34342575
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-08-3"; volume: "10. pii: 69549. doi"; pages: " 
            10.7554/eLife.69549"
COMMENT     Sequence Label: FRP2374
FEATURES             Location/Qualifiers
     source          1..10900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        116..466
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     CDS             467..1801
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     primer_bind     2474..2496
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2488..2505
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2489..2505
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2512..2531
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2512..2530
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3442..3461)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             3987..4007
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6319..6568
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             8002..8022
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8065..8254
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(8340..8358)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8379..8395)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8379..8395)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8392..8414)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8403..8419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(8972..8989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9143..9731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9223..9242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9910..10701,10702..10770))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10533..10552
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10771..10875)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaaac acacaccata ccttcccata tgtcctggcg cgcccttagc tctcattatt
      121 ttttgctttt tctcttgagg tcacatgatc gcaaaatggc aaatggcacg tgaagctgtc
      181 gatattgggg aactgtggtg gttggcaaat gactaattaa gttagtcaag gcgccatcct
      241 catgaaaact gtgtaacata ataaccgaag tgtcgaaaag gtggcacctt gtccaattga
      301 acacgctcga tgaaaaaaat aagatatata taaggttaag taaagcgtct gttagaaagg
      361 aagtttttcc tttttcttgc tctcttgtct tttcatctac tatttccttc gtgtaataca
      421 gggtcgtcag atacatagat acaattctat tacccccatc catacaatgc catctcattt
      481 cgatactgtt caactacacg ccggtcaaga gaaccctggt gacaatgctc acagatccag
      541 agctgtacca atttacgcca ccacttctta tgttttcgaa aactctaagc atggttcgca
      601 attgtttggt ttagaagttc caggttacgt ctattcccgt ttccaaaacc caaccagtaa
      661 tgttttggaa gaaagaattg ctgctttaga aggtggtgct gctgctttgg ctgtttcctc
      721 cggtcaagcc gctcaaaccc ttgccatcca aggtttggca cacactggtg acaacatcgt
      781 ttccacttct tacttatacg gtggtactta taaccagttc aaaatctcgt tcaaaagatt
      841 tggtatcgag gctagatttg ttgaaggtga caatccagaa gaatttgaaa aggtctttga
      901 tgaaagaacc aaggctgttt atttggaaac cattggtaat ccaaagtaca atgttccgga
      961 ttttgaaaaa attgttgcaa ttgctcacaa acacggtatt ccagttgtcg ttgacaacac
     1021 atttggtgcc ggtggttact tctgtcagcc aattaaatac ggtgctgata ttgtaacaca
     1081 ttctgctacc aaatggattg gtggtcatgg tactactatc ggtggtatta ttgttgactc
     1141 tggtaagttc ccatggaagg actacccaga aaagttccct caattctctc aacctgccga
     1201 aggttatcac ggtactatct acaatgaagc ctacggtaac ttggcataca tcgttcatgt
     1261 tagaactgaa ctattaagag atttgggtcc attgatgaac ccatttgcct ctttcttgct
     1321 actacaaggt gttgaaacat tatctttgag agctgaaaga cacggtgaaa atgcattgaa
     1381 gttagccaaa tggttagaac aatccccata cgtatcttgg gtttcatacc ctggtttagc
     1441 atctcattct catcatgaaa atgctaagaa gtatctatct aacggtttcg gtggtgtctt
     1501 atctttcggt gtaaaagact taccaaatgc cgacaaggaa actgacccat tcaaactttc
     1561 tggtgctcaa gttgttgaca atttaaagct agcctctaac ttggccaatg ttggtgatgc
     1621 caagacctta gtcattgctc catacttcac tacccacaaa caattaaatg acaaagaaaa
     1681 gttggcatct ggtgttacca aggacttaat tcgtgtctct gttggtatcg aatttattga
     1741 tgacattatt gcagacttcc agcaatcttt tgaaactgtt ttcgctggcc aaaaaccatg
     1801 agtgtgcgta atgagttgta aaattatgta taaacctact ttctctcaca agtactatac
     1861 ttttataaaa cgaactttat tgaaatgaat atcctttttt tcccttgtta catgtcgtga
     1921 ctcgtacttt gaacctaaat tgttctaaca tcaaagaaca gtgttaattc gcagtcgaga
     1981 agaaaaatat ggtgaacaag actcatctac ttcatgagac tactttacgc ctcctataaa
     2041 gctgtcacac tggataaatt tattgtagga ccaagttaca aaagaggatg atggaggttt
     2101 ctttacaata aagaagcaca tgtgtgttaa cgtttttagt atttgcttgt tatgtaaatc
     2161 aggaaaactt cgcgggattt ggttggatgc tactttccat acaataaata ttatagatca
     2221 aaaaagccaa attacaagta aagattagta aagctgttgc aattccatcg ttgataaaaa
     2281 tgttagttat taaatataaa agtcagaata ggtgaacttg gatttaattg ttggcatttc
     2341 gttgctgcta gaggccataa tattagatag ccaggacata ctaggtctcc tcgtggtata
     2401 ggaatccata aaatggaatt ggtgacgatc ggatgtgctg caaggcgatt aagttgggta
     2461 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     2521 tcactatagg gcgaattgga gaaacagaat aatatataaa cctgtataat ataaccttga
     2581 agactatatt tcttttcgcg gccgcctata gggcgaattg gagctccacc gcggtggctc
     2641 tagaagtcga acaagaagca ggcaaagttt agagcactgc ccctccgcac tcaaaaaaga
     2701 aaaaactagg aggaaaataa aattctcaac cacacaaaca cataaacaca tacaaataca
     2761 aatacaagct tatttacttg acatcgcgcg atcttccact attcagcgcc gtccgccctc
     2821 tctcgtgttt tttgtttacg cgacaactat gcgaaatccg gagcaacggg caaccgtttg
     2881 gggaaagacc acacccacgc gcgatcgcca tggcaacgag gtcgcacacg ccccacaccc
     2941 agacctccct gcgagcgggc atgggtacaa tgtccccgtt gccacagaga ccacttcgta
     3001 gcacagcgca gagcgtagcg tgttgttgct gctgacaaaa gaaaattttt cttagcaaag
     3061 caaaggaggg gaagcacggg cagatagcac cgtaccatac ccttggaaac tcgaaatgaa
     3121 cgaagcagga aatgagagaa tgagagtttt gtaggtatat atagcggtag tgtttgcgcg
     3181 ttaccatcat cttctggatc tatctattgt tcttttcctc atcactttcc cctttttcgc
     3241 tcttcttctt gtcttttatt tctttctttt ttttaattgt tccctcgatt ggctatctac
     3301 caaagaatcc aaacttaata cacgtattta tttgtccaat taccggatcc cccgggctgc
     3361 aggaattcat gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta
     3421 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc
     3481 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg
     3541 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt
     3601 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa
     3661 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag
     3721 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg
     3781 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa
     3841 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
     3901 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcatt tgcggattag
     3961 aaaaacaact taaatgtgaa agtgccccca agaaaaagag aaaggtggca gaagccgccg
     4021 caaaggaagc ggcagcaaaa gaggctgcgg ccaaggaagc tgctgccaag gcgctagaag
     4081 cagaggcggc cgcaaaggag gcggccgcga aggaagcggc tgcgaaggag gcagcggcaa
     4141 aggcgatgac tgccagcgtt tcgaatacgc agaataagct gaatgagctt ctcgatgcca
     4201 tcagacagga gtttctccaa gtctcacaag aggcaaatac ctaccgtctt caaaaccaaa
     4261 aggattacga tttcaaaatg aaccagcagc tggctgagat gcagcagata agaaacaccg
     4321 tctacgaact ggaactaact cacaggaaaa tgaaggacgc gtacgaagaa gagatcaagc
     4381 acttgaaact agggctggag caaagagacc atcaaattgc atctttgacc gtccagcaac
     4441 agcggcaaca gcaacagcag caacaggtcc agcagcattt acaacagcaa cagcagcagc
     4501 tagccgctgc atctgcatct gttccagttg cgcaacaacc accggctact acttcggcca
     4561 ccgccactcc agcagcaaac acaactactg gttcgccatc ggccttccca gtacaagcta
     4621 gccgtcctaa tctggttggc tcacagttgc ctaccaccac tttgcctgtg gtgtcctcaa
     4681 acgcccaaca acaactacca caacagcaac tgcaacagca gcaacttcaa caacagcaac
     4741 cacctcccca ggtttccgtg gcaccattga gtaacacagc catcaacgga tctcctactt
     4801 ctaaagagac cactacttta ccctctgtca aggcacctga atctacgttg aaagaaactg
     4861 aaccggaaaa taataatacc tcgaagataa atgacaccgg atccgccacc acggccacca
     4921 ctaccaccgc aactgaaact gaaatcaaac ctaaggagga agacgccacc ccggctagtt
     4981 tgcaccagga tcactactta gtcccttata atcaaagagc aaaccactct aaacctatcc
     5041 cacctttcct tttggatcta gattcccagt ctgttcccga tgctctgaag aagcaaacaa
     5101 atgattatta tattttatac aacccggcac taccaagaga aattgacgtt gagttacaca
     5161 aatctttgga tcatacttca gttgtttgtt gcgtgaagtt cagtaacgat ggtgaatact
     5221 tagccacagg ctgcaacaaa actactcaag tgtatcgcgt ttcagatggt tctctggtgg
     5281 cccgtctatc tgacgattct gctgccaata accatcgaaa ttcgatcact gaaaataaca
     5341 ccaccacgtc cacggataac aatacaatga caaccactac taccaccaca attactacca
     5401 cagcgatgac ttcggcagca gaattggcaa aagatgtgga aaacctgaac acttcgtctt
     5461 ccccatcatc cgacttgtat atccgttcag tgtgtttttc tccagatggg aaatttttgg
     5521 caacaggtgc tgaagacaga ctgattagaa tttgggatat tgaaaataga aagattgtta
     5581 tgattcttca aggccacgaa caagatattt attcattgga ctactttccc tcaggtgaca
     5641 aattagtctc cggttctggt gaccgtaccg ttcgtatttg ggacttacgt acaggccagt
     5701 gttcattgac tttatccatt gaagatggtg ttaccaccgt cgctgtatca ccaggtgatg
     5761 gtaaatacat cgctgctggt tctctagatc gtgctgtgag agtttgggat tccgagaccg
     5821 gattcttggt ggaaagacta gattcggaaa acgaatccgg tacaggccac aaggactctg
     5881 tttatagcgt tgtcttcact agagatggac aaagcgttgt atccggctca ttagatagat
     5941 ctgttaagct ctggaatttg cagaatgcaa acaacaagag cgattcgaaa actccaaatt
     6001 ccggcacttg tgaagttacg tatatcgggc ataaagactt tgtattgtcc gtggccacca
     6061 cacaaaatga tgagtacatc ttgtccggtt ccaaagatcg tggtgtcctg ttttgggata
     6121 agaaatccgg caatccgtta ttgatgttgc aaggtcatag gaattcagtt atatctgtgg
     6181 ctgtggcaaa cgggtctccg ctgggtccag aatataacgt ttttgctact ggtagcggtg
     6241 attgtaaagc aaggatttgg aagtataaaa aaatagcgcc aaattaactc gagacaggcc
     6301 ccttttcctt tgtctatatc atgtaattag ttatgtcacg cttacattca cgccctcctc
     6361 ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt
     6421 atttttttta atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt
     6481 ttttctgtac aaacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt
     6541 tttgggacgc tcgaaggctt taatttgcaa ggttcgcagt ttacactctc atcgtcgctc
     6601 tcatcatcgc ttccgttgtt gttttcctta gtagcgtctg ctttctagac agttcgagtt
     6661 tatcattatc aatactgcca tttcaaagaa tacgtaaata attaatagta gtgattttcc
     6721 taactttatt tagtcaaaaa attagcctct atcattgata gagtgtctgg gtgatctatc
     6781 attgatagag catccactaa ttttaattct gctgtaaccc gtacatgccc aaaatagggg
     6841 gcgggttaca cagaatctat cattgataga gtgtctgggt gatctatcat tgatagagca
     6901 tccactaaat ataatggagc tctatcattg atagagcatc cactaaaaaa aagaatccca
     6961 gcaccaaaat attgttttct tcaccaacca tcagttcata ggtccattct cttagcgcaa
     7021 ctacagagaa caggggcaca aacaggcaaa aaacgggcac aacctcaatg gagtgatgca
     7081 acctgcctgg agtaaatgat gacacaaggc aattgaccca cgcatgtatc tatctcattt
     7141 tcttacacct tctattacct tctgctctct ctgatttgga aaaagctgaa aaaaaaggtt
     7201 gaaaccagtt ccctgaaatt attcccctat ctatcattga tagatataaa tatctatcat
     7261 tgatagagta attctgtaaa tctatttctt aaacttctta aattctactt ttatagttag
     7321 tctttttttt agttttaaaa caccaagaac ttagtttcga ataaacacac ataaacaaac
     7381 aaaatgtcca ggttggacaa gtccaaggtt attaactctg ccttggagtt gttgaacgaa
     7441 gttggtattg aaggtttgac cactagaaag ttggctcaaa aattgggtgt tgaacaacct
     7501 accttgtact ggcatgttaa gaacaaaaga gctttgttgg acgctttggc cattgaaatg
     7561 ttggatagac atcataccca tttctgtcca ttggaaggtg aatcatggca agatttcttg
     7621 agaaacaacg ccaagtcttt cagatgtgct ttgttgtctc atagagatgg tgctaaagtt
     7681 cacttgggta ctagaccaac tgaaaagcaa tacgaaactt tggaaaacca gttggctttc
     7741 ttgtgtcaac aaggtttctc tttagaaaac gccttgtatg ctttgtctgc tgttggtcat
     7801 tttaccttgg gttgtgtttt ggaagatcaa gaacatcaag tcgccaaaga agaaagggaa
     7861 actccaacta ctgattctat gccaccatta ttgagacaag ccattgagtt gtttgatcat
     7921 caaggtgctg aaccagcttt tttgtttggt ttggaattga tcatctgcgg tttggagaaa
     7981 caattgaagt gtgaatctgc ccctaagaag aagagaaagg tttgaattag ttatgtcacg
     8041 cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc
     8101 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
     8161 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
     8221 aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgggccg tatttacaga
     8281 taacgatgtc gttattatat atatatatat atattgtcac ttgtacccag cttttgttcc
     8341 ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga
     8401 aattgttatc cgctcacaac agctgtcaga tatagtcgga ttgccctttt aagcaatcaa
     8461 tagtgtttta tttgcaacaa tgtcgtcata gtttaatatg tcctataaga tgttaacttg
     8521 ctcaacattc aacaaagttt ggtctcttgg cccagttggt taaggcaccg tgctaataac
     8581 gcggggatca gcggttcgat cccgctagag accatttatt tttaaattcg accgtctgac
     8641 agacggtgca cttattactt aatttttttt tttttttaat cctcgttaaa ctgagtacat
     8701 aatcagcaac aatataatat aaaagttttg cttttttatg tacgtacaat aggggtactt
     8761 aaataaataa ataaacaata aaacaatcaa taaataaata atatacgatc ataaattacg
     8821 agatatattt catagtcttg acttctccag ccgaacgtca ttaaatttaa ggcagaaaag
     8881 ataaacaagg gcgcgccaga gcctgcaggc attaatgaat cggccaacgc gcggggagag
     8941 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9001 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9061 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9121 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9181 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9241 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9301 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9361 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9481 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9541 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9601 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9661 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9721 aaggatctca agaagatcct ttgatctttt gtttaaacct acggggtctg acgctcagtg
     9781 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9841 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9901 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9961 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10021 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10081 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10141 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10201 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10261 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10321 caaaaaagcg gttagctcct tcggtcctcc gatggttgtc agaagtaagt tggccgcagt
    10381 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10441 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10501 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10561 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10621 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10681 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10741 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10801 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct
//
