LOCUS       sequence-246172-       11628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescently tagged p65 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Feldman D, Singh A, Schmid-Burgk JL, Carlson RJ, Mezger A, Garrity 
            AJ, Zhang F, Blainey PC
  TITLE     Optical Pooled Screens in Human Cells.
  JOURNAL   Cell. 2019 Oct 17;179(3):787-799.e17. doi: 
            10.1016/j.cell.2019.09.016.
  PUBMED    31626775
REFERENCE   2  (bases 1 to 11628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.09"; journalName: "Cell"; date: "2019-10-17- 
            17"; volume: "179"; issue: "3"; pages: "787-799.e17"
COMMENT     Sequence Label: p65-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..11628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1853..3031
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2084..3022
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2979..2999
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3038..3047
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4325..4696
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             4721..5422
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEEL
                     NLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGA
                     SLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTF
                     KWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKA
                     FTDVMGMDELYK"
     regulatory      4970..4979
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5432..5458
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5486..6074
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5539..5559)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5957..5968)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6077..6093)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6078..6094)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6599..6779
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6805..6822)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6811..7035
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7081..7509
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7168..7187)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7378..7399
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7518..7538)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7523..7852
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7703..7838
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7765..7784
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7900..7947
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7966..8340
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8470..8591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8507..8526)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8561..8580
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8640..8656)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8640..8656)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8653..8675)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8664..8680
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8688..8694,8695..8712,8713..8718))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8733..8754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8871..8888)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9042..9630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9122..9141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9801..10592,10593..10661))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10424..10443
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10662..10766)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10841..10860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11032..11411
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11413..11611
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11566..11586
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt taattagcta gctgcaaaga tggataaagt tttaaacaga
     1801 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
     1861 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     1921 cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     1981 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     2041 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     2101 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     2161 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     2221 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     2281 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     2341 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     2401 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     2461 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     2521 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     2581 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     2641 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     2701 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     2761 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     2821 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     2881 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     2941 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     3001 aagttttttt cttccatttc aggtgtcgtg atgtacagcc accatggacg aactgttccc
     3061 cctcatcttc ccggcagagc cagcccaggc ctctggcccc tatgtggaga tcattgagca
     3121 gcccaagcag cggggcatgc gcttccgcta caagtgcgag gggcgctccg cgggcagcat
     3181 cccaggcgag aggagcacag ataccaccaa gacccacccc accatcaaga tcaatggcta
     3241 cacaggacca gggacagtgc gcatctccct ggtcaccaag gaccctcctc accggcctca
     3301 cccccacgag cttgtaggaa aggactgccg ggatggcttc tatgaggctg agctctgccc
     3361 ggaccgctgc atccacagtt tccagaacct gggaatccag tgtgtgaaga agcgggacct
     3421 ggagcaggct atcagtcagc gcatccagac caacaacaac cccttccaag ttcctataga
     3481 agagcagcgt ggggactacg acctgaatgc tgtgcggctc tgcttccagg tgacagtgcg
     3541 ggacccatca ggcaggcccc tccgcctgcc gcctgtcctt tctcatccca tctttgacaa
     3601 tcgtgccccc aacactgccg agctcaagat ctgccgagtg aaccgaaact ctggcagctg
     3661 cctcggtggg gatgagatct tcctactgtg tgacaaggtg cagaaagagg acattgaggt
     3721 gtatttcacg ggaccaggct gggaggcccg aggctccttt tcgcaagctg atgtgcaccg
     3781 acaagtggcc attgtgttcc ggacccctcc ctacgcagac cccagcctgc aggctcctgt
     3841 gcgtgtctcc atgcagctgc ggcggccttc cgaccgggag ctcagtgagc ccatggaatt
     3901 ccagtacctg ccagatacag acgatcgtca ccggattgag gagaaacgta aaaggacata
     3961 tgagaccttc aagagcatca tgaagaagag tcctttcagc ggacccaccg acccccggcc
     4021 tccacctcga cgcattgctg tgccttcccg cagctcagct tctgtcccca agccagcacc
     4081 ccagccctat ccctttacgt catccctgag caccatcaac tatgatgagt ttcccaccat
     4141 ggtgtttcct tctgggcaga tcagccaggc ctcggccttg gccccggccc ctccccaagt
     4201 cctgccccag gctccagccc ctgcccctgc tccagccatg gtatcagctc tggcccaggc
     4261 cccagcccct gtcccagtcc tagccccagg ccctcctcag gctgtggccc cacctgcccc
     4321 caagcccacc caggctgggg aaggaacgct gtcagaggcc ctgctgcagc tgcagtttga
     4381 tgatgaagac ctgggggcct tgcttggcaa cagcacagac ccagctgtgt tcacagacct
     4441 ggcatccgtc gacaactccg agtttcagca gctgctgaac cagggcatac ctgtggcccc
     4501 ccacacaact gagcccatgc tgatggagta ccctgaggct ataactcgcc tagtgacagg
     4561 ggcccagagg ccccccgacc cagctcctgc tccactgggg gccccggggc tccccaatgg
     4621 cctcctttca ggagatgaag acttctcctc cattgcggac atggacttct cagccctgct
     4681 gagtcagatc agctccggag gcggctccgg tggaggatcc gtgagcaagg gcgaggagga
     4741 taacgcctct ctcccagcga cacatgagtt acacatcttt ggctccatca acggtgtgga
     4801 ctttgacatg gtgggtcagg gcaccggcaa tccaaatgat ggttatgagg agttaaacct
     4861 gaagtccacc aagggtgacc tccagttctc cccctggatt ctggtccctc atatcgggta
     4921 tggcttccat cagtacctgc cctaccctga cgggatgtcg cctttccagg ccgccatggt
     4981 agatggctcc ggataccaag tccatcgcac aatgcagttt gaagatggtg cctcccttac
     5041 tgttaactac cgctacacct acgagggaag ccacatcaaa ggagaggccc aggtgaaggg
     5101 gactggtttc cctgctgacg gtcctgtgat gaccaactcg ctgaccgctg cggactggtg
     5161 caggtcgaag aaaacttacc ccaacgacaa aaccatcatc agtaccttta agtggagtta
     5221 caccactgga aatggcaagc gctaccggag cactgcgcgg accacctaca cctttgccaa
     5281 gccaatggcg gctaactatc tgaagaacca gccgatgtac gtgttccgta agacggagct
     5341 caagcactcc aagaccgagc tcaacttcaa ggagtggcaa aaggccttta ccgatgtgat
     5401 gggcatggac gagctgtata agggaagatc ctacccatac gatgttccag attacgcttg
     5461 agaattcgat atcaagctta tcggtaatca acctctggat tacaaaattt gtgaaagatt
     5521 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5581 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5641 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5701 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5761 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5821 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5881 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5941 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6001 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6061 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     6121 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     6181 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     6241 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6301 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     6361 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     6421 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     6481 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     6541 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     6601 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6661 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6721 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6781 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6841 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6901 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6961 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7021 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     7081 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7141 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7201 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7261 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7321 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7381 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7441 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7501 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7561 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7621 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7681 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7741 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     7801 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     7861 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     7921 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     7981 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     8041 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     8101 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     8161 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     8221 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     8281 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     8341 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     8401 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     8461 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8521 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8581 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     8641 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8701 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8761 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8821 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8881 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8941 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9001 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9061 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9481 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9541 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9601 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9661 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9721 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9781 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9841 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9901 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9961 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10021 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10081 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10141 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10201 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10261 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10321 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10381 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10441 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10501 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10561 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10621 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10681 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10741 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
    10801 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
    10861 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
    10921 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
    10981 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
    11041 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    11101 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
    11161 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
    11221 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
    11281 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
    11341 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
    11401 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
    11461 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
    11521 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
    11581 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtact
//
