LOCUS       sequence-246173-        6149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the red genetically encoded dopamine sensor RdLight1 in 
            mammalian neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Patriarchi T, Mohebi A, Sun J, Marley A, Liang R, Dong C, Puhger K, 
            Mizuno GO, Davis CM, Wiltgen B, von Zastrow M, Berke JD, Tian L
  TITLE     An expanded palette of dopamine sensors for multiplex imaging in 
            vivo.
  JOURNAL   Nat Methods. 2020 Nov;17(11):1147-1155. doi: 
            10.1038/s41592-020-0936-3. Epub 2020 Sep 7.
  PUBMED    32895537
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0936-3"; journalName: "Nat Methods"; date: 
            "2020-11"; volume: "17"; issue: "11"; pages: "1147-1155"
COMMENT     Sequence Label: pAAV-hSynapsin1-RdLight1
FEATURES             Location/Qualifiers
     source          1..6149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   162..291
                     /label=AAV2 ITR
     promoter        373..820
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    2922..3510
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2975..2995)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3393..3404)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3513..3529)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3514..3530)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3559..3680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3570..3589)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3624..3643
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4034..4622)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4114..4133)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4793..5584,5585..5653))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5416..5435
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5654..5758)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(5784..6149,1..90))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5921..5942)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(6133..6149,1..3)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
       61 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
      121 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctgcgcgct cgctcgctca
      181 ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga
      241 gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc ttgtagttaa
      301 tgattaaccc gccatgctac ttatctacgt agccatgctc taggaagatc tctgcagagg
      361 gccctgcgta tgagtgcaag tgggttttag gaccaggatg aggcggggtg ggggtgccta
      421 cctgacgacc gaccccgacc cactggacaa gcacccaacc cccattcccc aaattgcgca
      481 tcccctatca gagaggggga ggggaaacag gatgcggcga ggcgcgtgcg cactgccagc
      541 ttcagcaccg cggacagtgc cttcgccccc gcctggcggc gcgcgccacc gccgcctcag
      601 cactgaaggc gcgctgacgt cactcgccgg tcccccgcaa actccccttc ccggccacct
      661 tggtcgcgtc cgcgccgccg ccggcccagc cggaccgcac cacgcgaggc gcgagatagg
      721 ggggcacggg cgcgaccatc tgcgctgcgg cgccggcgac tcagcgctgc ctcagtctgc
      781 ggtgggcagc ggaggagtcg tgtcgtgcct gagagcgcag tcgaattcaa gctgctagca
      841 aggatccacc ggtgcgcgcc atgaagacga tcatcgccct gagctacatc ttctgcctgg
      901 tgttcgccga ctacaaggac gatgatgacg ccatgaggac tctgaacacc tctgccatgg
      961 acgggactgg gctggtggtg gagagggact tctctgttcg tatcctcact gcctgtttcc
     1021 tgtcgctgct catcctgtcc acgctcctgg ggaacacgct ggtctgtgct gccgttatca
     1081 ggttccgaca cctgcggtcc aaggtgacca acttctttgt catctccttg gctgtgtcag
     1141 atctcttggt ggccgtcctg gtcatgccct ggaaggcagt ggctgagatt gctggcttct
     1201 ggccctttgg gtccttctgt aacatctggg tggcctttga catcatgtgc tccactgcat
     1261 ccatcctcaa cctctgtgtg atcagcgtgg acaggtattg ggctatctcc agccctgaac
     1321 ggtatgagag aaagatgacc cccaaggcag ccttcatcct gatcagtgtg gcatggacct
     1381 tgtctgtact catctccttc atcccagtgc agctcagctg gcacaaggca aaacccacaa
     1441 gcccctctga tggaaatgcc acttccctgg ctgagaccat agacaactgt gactccagcc
     1501 tcagcaggac atatgccatc tcatcctctg taatcagctt ttacatccct gtggccatca
     1561 tgattgtcac ctacaccagg atctacagga ttgctcagaa acagctctcc tcctttcatg
     1621 tgtcagagcg gatgtatcca gaagatggag cgttgaagtc agagatcaaa aaaggactgc
     1681 ggttgaagga tggcggacat tatgcagctg aggtcaagac tacatataaa gctaaaaaac
     1741 ccgttcagct tccgggtgcg tatatcgtag atattaaact cgacattgtt tctcacaacg
     1801 aggattatac aatcgtcgag caatgcgaaa gggcagaagg acgacactcc accgggggaa
     1861 tggatgagct ttataaagga ggtactggtg gcagccttgt ctcaaagggg gaggaagaca
     1921 atatggcaat aattaaagag ttcatgagat tcaaagtaca catggaggga agcgtcaatg
     1981 gtcacgagtt cgagatcgaa ggggaagggg agggacgccc ttatgaggcc tttcagaccg
     2041 ccaaattgaa agtaacaaag ggtggacccc ttccatttgc gtgggacatc ctctcccctc
     2101 agtttatgta tggtagtaag gcttatataa agcatccggc tgatatacca gattacttta
     2161 aactttcctt cccggaaggg tttcgctggg aacgcgtcat gaatttcgag gatgggggca
     2221 ttatacacgt caatcaagat agctccttgc aagatggtgt ctttatctat aaggtaaagc
     2281 tgagagggac aaattttcca ccggatggac cagtaatgca gaagaaaact atggggtggg
     2341 aagcacggaa ggacgacctt aaaagagaaa ctaaagtcct gaagactctg tcggtgatca
     2401 tgggtgtgtt tgtgtgctgt tggctacctt tcttcatctt gaactgcatt ttgcccttct
     2461 gtgggtctgg ggagacgcag cccttctgca ttgattccaa cacctttgac gtgtttgtgt
     2521 ggtttgggtg ggctaattca tccttgaacc ccatcattta tgcctttaat gctgattttc
     2581 ggaaggcatt ttcaaccctc ttaggatgct acagactttg ccctgcgacg aataatgcca
     2641 tagagacggt gagtatcaat aacaatgggg ccgcgatgtt ttccagccat catgagccac
     2701 gaggctccat ctccaaggag tgcaatctgg tttacctgat cccacatgct gtgggctcct
     2761 ctgaggacct gaaaaaggag gaggcagctg gcatcgccag acccttggag aagctgtccc
     2821 cagccctatc ggtcatattg gactatgaca ctgacgtctc tctggagaag atccaaccca
     2881 tcacacaaaa cggtcagcac ccaacctgaa agcttatcga taatcaacct ctggattaca
     2941 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     3001 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     3061 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     3121 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     3181 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     3241 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     3301 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     3361 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     3421 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     3481 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga cccgggcggc
     3541 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     3601 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3661 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     3721 gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
     3781 aggatcttcc tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac
     3841 aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcggtc gttcggctgc
     3901 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3961 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaagc caggaaccgt aaaaaggccg
     4021 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4201 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4441 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4501 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4561 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4861 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5101 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5401 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5641 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5761 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     5821 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     5881 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     5941 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     6001 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     6061 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     6121 ggcgagaaag gaagggaaga aagcgaaag
//
