LOCUS       sequence-246177-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry plasmid for guide cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Feldman D, Singh A, Schmid-Burgk JL, Carlson RJ, Mezger A, Garrity 
            AJ, Zhang F, Blainey PC
  TITLE     Optical Pooled Screens in Human Cells.
  JOURNAL   Cell. 2019 Oct 17;179(3):787-799.e17. doi: 
            10.1016/j.cell.2019.09.016.
  PUBMED    31626775
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.09"; journalName: "Cell"; date: "2019-10-17- 
            17"; volume: "179"; issue: "3"; pages: "787-799.e17"
COMMENT     Sequence Label: CROPseq-Guide-Zeo
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             97..277
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    355..476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(392..411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     446..465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      516..651
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(671..690)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(672..690)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(700..717)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(700..716)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(709..731)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      858..1313
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(945..964)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1155..1176
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1339..1443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1444..1512,1513..2304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1662..1681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2475..3063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2964..2983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3217..3234
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3351..3372
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3387..3392,3393..3410,3411..3417)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3425..3441
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3430..3452
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3449..3465
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3449..3465
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3484..3504
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3486..3504
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3532..3758
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3759..3939
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3986..4111
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4604..4837
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5022..5066
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5247..5364
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5503..6681
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5734..6672
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6629..6649
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6688..6697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6694..7068
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    7084..7672
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7137..7157)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7555..7566)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7828..8076
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7828..7848
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7999..8018
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        join(9958..9989,1..44)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt aagcttggcg
       61 taactagatc ttgagacact gctttttgct tgtactgggt ctctctggtt agaccagatc
      121 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      181 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      241 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
      301 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
      361 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      421 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
      481 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      541 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      601 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
      661 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
      721 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      781 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      841 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      901 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      961 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     1021 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     1081 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     1141 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     1201 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     1261 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     1321 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1381 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1441 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1501 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1561 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1621 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1681 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1741 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1801 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1861 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1921 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1981 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     2041 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     2101 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     2161 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     2221 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2281 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     2341 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2401 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2461 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2521 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2581 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2641 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2701 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2761 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2821 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2881 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2941 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3001 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3061 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3121 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3181 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3241 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3301 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     3361 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     3421 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     3481 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     3541 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     3601 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     3661 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     3721 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     3781 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     3841 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3901 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     3961 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     4021 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     4081 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     4141 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     4201 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     4261 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     4321 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     4381 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     4441 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     4501 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     4561 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     4621 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     4681 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     4741 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     4801 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     4861 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     4921 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     4981 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     5041 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     5101 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     5161 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagaatcttg
     5221 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     5281 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     5341 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     5401 cgccggctcg agggggcccg ggtgcaaaga tggataaagt tttaaacaga gaggaatctt
     5461 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     5521 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5581 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5641 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5701 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     5761 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     5821 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     5881 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     5941 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     6001 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     6061 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     6121 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     6181 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     6241 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     6301 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     6361 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     6421 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     6481 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     6541 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     6601 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     6661 cttccatttc aggtgtcgtg acgtacggcc accatggcca agttgaccag tgccgttccg
     6721 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     6781 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     6841 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     6901 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     6961 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     7021 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaac gcgttaagtc
     7081 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7141 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7201 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7261 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7321 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7381 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7441 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7501 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7561 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7621 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     7681 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7741 gactggaagg gctaattcac tcccaacgaa gacaagattt caccattatc gtttcagacc
     7801 cacctcccaa ccccgagggg acccagagag ggcctatttc ccatgattcc ttcatatttg
     7861 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     7921 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     7981 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     8041 ttggctttat atatcttgtg gaaaggacga aacaccggag acggttgtaa atgagcacac
     8101 aaaatacaca tgctaaaata ttatattcta tgacctttat aaaatcaacc aaaatcttct
     8161 ttttaataac tttagtatca ataattagaa tttttatgtt cctttttgca aacttttaat
     8221 aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc gcgttgtttt cttcaccttt
     8281 aataatagct actccaccac ttgttcctaa gcggtcagct cctgcttcaa tcattttttg
     8341 agcatcttca aatgttctaa ctccaccagc tgctttaact aaagcattgt ctttaacaac
     8401 tgacttcatt agtttaacat cttcaaatgt tgcacctgat tttgaaaatc ctgttgatgt
     8461 tttaacaaat tctaatccag cttcaacagc tatttcacaa gctttcatga tttcttcttt
     8521 tgttaataaa caattttcca taatacattt aacaacatgt gatccagctg ctttttttac
     8581 agctttcatg tcttctaaaa ctaattcata atttttgtct tttaatgcac caatatttaa
     8641 taccatatca atttctgttg caccatcttt aattgcttca gaaacttcga atgcttttgt
     8701 agctgttgtg catgcaccta gaggaaaacc tacaacattt gttattccta catttgtgcc
     8761 ttttaataat tctttacaat agcttgttca atatgaatta acacaaactg ttgcaaaatc
     8821 aaattcaatt gcttcatcac ataattgttt aatttcagct ttcgtagcat cttgttttaa
     8881 taatgtgtga tctatatatt tgtttagttt cattttttct cctatatatt catttttaat
     8941 tttaattctt taataatttc gtctacttta actttagcgt tttgaacaga ttcaccaaca
     9001 cctataaaat aaatttttag tttaggttca gttccacttg ggcgaacagc aaatcatgac
     9061 ttatcttcta aataaaattt tagtaagtct tgtcctggca tattatacat tccatcgatg
     9121 tagtcttcaa cattaacaac tttaagtcca gcaatttgag ttaagggtgt tgctctcaat
     9181 gatttcatta atggttcaat ttttaatttc ttttcttctg gtttaaaatt caagtttaaa
     9241 gtgaaagtgt aatatgcacc catttcttta aataaatctt ctaaatagtc tactaatgtt
     9301 ttattttgtt ttttataaaa tcaagcagcc tctgctatta atatagaagc ttgtattcca
     9361 tctttatctc tagctgagtc atcaattaca tatccataac tttcttcata agcaaaaaca
     9421 aaatttaatc cgttatcttc ttctttagca atttctctac ccattcattt aaatccagtt
     9481 aaagttttta caatattaac tccatatttt tcatgagcga ttctatcacc caaatcactt
     9541 gttacaaaac ttgaatatag agccggattt tttggaatgc tatttaagcg ttttagattt
     9601 gataattttc aatcaattaa aattggtcct gtttgatttc catctaatct tacaaaatga
     9661 ccatcatgtt ttattgccat tccaaatctg tcagcatctg ggtcattcat aataataata
     9721 tctgcatcat gtttaatacc atattcaagc ggtatttttc atgcaggatc aaattctgga
     9781 tttggattta caacattttt aaatgtttca tcttcaaatg catgctcttc aacctcaata
     9841 acgttatatc ctgattcacg taatattttt ggggtaaatt tagttcctgt tccattaact
     9901 gcgctaaaaa taatttttaa atctttttta gcttcttgct cttttttgta cgtctctgtt
     9961 ttagagctag aaatagcaag ttaaaataa
//
