LOCUS       sequence-246185-        9131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a 
            sgRNA controlled constitutively from PpflB S. aureus promoter to 
            clone a guide between two BsaI sites and oriT on pLZ12 vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Depardieu F, Bikard D
  TITLE     Gene silencing with CRISPRi in bacteria and optimization of dCas9 
            expression levels.
  JOURNAL   Methods. 2019 Aug 1. pii: S1046-2023(18)30497-3. doi: 
            10.1016/j.ymeth.2019.07.024.
  PUBMED    31377338
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2019 Aug 1. pii: S1046-2023(18)30497-3. doi: 
            10.1016/j.ymeth.2019.07.024."
COMMENT     Sequence Label: pFD115
FEATURES             Location/Qualifiers
     source          1..9131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        44..119
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     oriT            294..403
                     /note="incP origin of transfer"
     CDS             complement(1279..1290)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(3916..4542)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     4447..4466
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             4735..8841
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4735..8838
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      8850..8921
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8937..8964
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 acaacaaaca tgctacaatt agttgagacc agtctaggtc tcggttttag agctagaaat
       61 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
      121 ttttttggag atctgtccat acccatggat tcttcgtctg tttctactcc atcagtctga
      181 cgaccaagag agccataaac accaatagcc ttaacatcat ccccatattt atccaatatt
      241 cgttccttaa tttcatgaac aatctgatat caagcttatc gataccgtcg acgccggcca
      301 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
      361 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
      421 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
      481 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaagcg
      541 ctggtaccca attcgcccta tagtgagtct tcttctctag tcattattat tggtccattc
      601 actattctca ttcccttttc agataatttt agatttgctt ttctaaataa gaatatttgg
      661 agagcaccgt tcttattcag ctattaataa ctcgtcttcc taagcatcct tcaatccttt
      721 taataacaat tatagcatct aatcttcaac aaactggccc gtttgttgaa ctactcttta
      781 ataaaataat ttttccgttc ccaattccac attgcaataa tagaaaatcc atcttcatcg
      841 gctttttcgt catcatctgt atgaatcaaa tcgccttctt ctgtgtcatc aaggtttaat
      901 tttttatgta tttcttttaa caaaccacca taggagatta accttttacg gtgtaaacct
      961 tcctccaaat cagacaaacg tttcaaattc ttttcttcat catcggtcat aaaatccgta
     1021 tcctttacag gatattttgc agtttcgtca attgccgatt gtatatccga tttatattta
     1081 tttttcggtc gattttttat taaaacgtct caaaatcgtt tctgagacgt tttagcgttt
     1141 atttcgttta gttatcggca taatcgttaa aacaggcgtt atcgtagcgt aaaagccctt
     1201 gagcgtagcg tggctttgca gcgaagatgt tgtctgttag attatgaaag ccgatgactg
     1261 aatgaaataa taagcgcagc gtccttctat ttcggttgga ggaggctcaa gggagtttga
     1321 gggaatgaaa ttccctcatg ggtttgattt taaaaattgc ttgcaatttt gccgagcggt
     1381 agcgctggaa aatttttgaa aaaaatttgg aatttggaaa aaaatggggg gaaaggaagc
     1441 gaattttgct tccgtactac gaccccccat taagtgccga gtgccaattt ttgtgccaaa
     1501 aacgctctat cccaactggc tcaagggttt gaggggtttt tcaatcgcca acgaatcgcc
     1561 aacgttttcg ccaacgtttt ttataaatct atatttaagt agctttattg ttgtttttat
     1621 gattacaaag tgatacacta attttataaa attatttgat tggagttttt taaatggtga
     1681 tttcagaatc gaaaaaaaga gttatgattt ctctgacaaa agagcaagat aaaaaattaa
     1741 cagatatggc gaaacaaaaa ggtttttcaa aatctgcggt tgcggcgtta gctatagaag
     1801 aatatgcaag aaaggaatca gaacaaaaaa aataagcgaa agctcgcgtt tttagaagga
     1861 tacgagtttt cgctacttgt ttttgataag gtaattatat catggctatt aaaaatacta
     1921 aagctagaaa ttttggattt ttattatatc ctgactcaat tcctaatgat tggaaagaaa
     1981 aattagagag tttgggcgta tctatggctg tcagtccttt acacgatatg gacgaaaaaa
     2041 aagataaaga tacatggaat agtagtgatg ttatacgaaa tggaaagcac tataaaaaac
     2101 cacactatca cgttatatat attgcacgaa atcctgtaac aatagaaagc gttaggaaca
     2161 agattaagcg aaaattgggg aatagttcag ttgctcatgt tgagatactt gattatatca
     2221 aaggttcata tgaatatttg actcatgaat caaaggacgc tattgctaag aataaacata
     2281 tatacgacaa aaaagatatt ttgaacatta atgattttga tattgaccgc tatataacac
     2341 ttgatgaaag ccaaaaaaga aaattgaaga atttactttt agatatagtg gatgactata
     2401 atttggtaaa tacaaaagat ttaatggctt ttattcgcct taggggagcg gagtttggaa
     2461 ttttaaatac gaatgatgta aaagatattg tttcaacaaa ctctagcgcc tttagattat
     2521 ggtttgaggg caattatcag tgtggatata gagcaagtta tgcaaaggtt cttgatgctg
     2581 aaacggggga aataaaatga caaacaaaga aaaagagtta tttgctgaaa atgaggaatt
     2641 aaaaaaagaa attaaggact taaaagagcg tattgaaaga tacagagaaa tggaagttga
     2701 attaagtaca acaatagatt tattgagagg agggattatt gaataaataa aagcccccct
     2761 gacgaaagtc caagggttta ttgttttcta aaatctgatt accaattaga atgaatattt
     2821 cccaaatatt aaataataaa acaaaaaaat taaaaaagtg tttccaccat tttttcaatt
     2881 tttttataat ttttttaatc tgttatttaa atagtttata gttaaattta cattttcatt
     2941 agtccattca atattctctc caagataact acgaactgct aacaaaattc tctccctatg
     3001 ttctaatgga gaagattcag ccactgcatt tcccgcaata tcttttggta tgattttacc
     3061 cgtgtccata gttaaaatca tacggcataa agttaatata gagttggttt catcatcctg
     3121 ataattatct attaattcct ctgacgaatc cataatggct cttctcacat cagaaaatgg
     3181 aatatcaggt agtaattcct ctaagtcata atttccgtat attcttttat tttttcgttt
     3241 tgcttggtaa agcattatgg ttaaatctga atttaattcc ttctgaggaa tgtatccttg
     3301 ttcataaagc tcttgtaacc attctccata aataaattct tgtttgggag gatgattcca
     3361 cggtaccatt tcttgctgaa taataattgt taattcaata tatcgtaagt tgcttttatc
     3421 tcctattttt tttgaaatag gtctaatttt ttgtataagt atttctttac tttgatctgt
     3481 caatggttca gatacgacga ctaaaaagtc aagatcacta tttggtttta gtccactctc
     3541 aactcctgat ccaaacatgt aagtaccaat aaggttattt tttaaatgtt tccgaagtat
     3601 ttttttcact ttattaattt gttcgtatgt attcaaatat atcctcctca ctattttgat
     3661 tagtacctat tttatatcca tagttgttaa ttaaataaac ttaatttagt ttatttatag
     3721 atttcattgg cttctaaatt ttttatctag ataataatta ttttagttaa ttttattcta
     3781 gattatatat gatatgatct ttcatttcca taaaactaaa gtaagtgtaa acctattcat
     3841 tgttttaaaa atatctcttg ccagtcacgt tacgttatta gttagttacc atcacggaaa
     3901 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
     3961 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     4021 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     4081 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     4141 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     4201 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     4261 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     4321 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     4381 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     4441 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
     4501 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct cctttttgtt
     4561 gacattatat cattgataga gttatttgtc aaactagttt tttatttgga tcccctcgag
     4621 ttcatgaaaa actaaaaaaa atattgacac tctatcattg atagagcata attaaaataa
     4681 gctctctatc attgatagag tgatatcgaa ttcggaggca tataggaggc aaaaatggat
     4741 aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc ggtgatcact
     4801 gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt
     4861 atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact
     4921 cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta
     4981 caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa
     5041 gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata
     5101 gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg
     5161 gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt
     5221 aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac
     5281 aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac
     5341 gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta
     5401 gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt
     5461 gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct
     5521 aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt
     5581 ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt
     5641 tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt
     5701 aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa
     5761 cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat
     5821 attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa
     5881 atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa
     5941 cggacctttg acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt
     6001 ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa
     6061 atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt
     6121 gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc
     6181 gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt
     6241 ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac
     6301 gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt
     6361 gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag
     6421 caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga
     6481 gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa
     6541 gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca
     6601 ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc
     6661 tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg
     6721 tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt
     6781 ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg
     6841 acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa
     6901 catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa
     6961 gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa
     7021 atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa
     7081 cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa
     7141 aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg
     7201 tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga tcacattgtt
     7261 ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa
     7321 aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat
     7381 tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa
     7441 gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt
     7501 gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa
     7561 tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta
     7621 gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat
     7681 catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca
     7741 aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt
     7801 gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc
     7861 atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta
     7921 atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca
     7981 gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca
     8041 ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt
     8101 aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca
     8161 gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag
     8221 ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta
     8281 gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt
     8341 ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa
     8401 ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat
     8461 gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat
     8521 aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta
     8581 gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga caaaccaata
     8641 cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct
     8701 gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt
     8761 ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg
     8821 agtcagctag gaggtgactg accaggcatc aaataaaacg aaaggctcag tcgaaagact
     8881 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     8941 caccttcggg tgggcctttc tgcgtttata atatactcct aaattaactt ttaaagcaat
     9001 gaaaatagtg aacattataa ctgttgtgta acagaatgca attagcatat tactgttaca
     9061 caaattagta cagtttctat gttttgacat acatttgatg aaaattgtac ataatttatg
     9121 tgaaaaaaat c
//
