LOCUS       sequence-246191-        2361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In-vitro-transcription of control RNA0 in NMR tube for ""Systems 
            NMR"" analysis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Nikolaev Y, Ripin N, Soste M, Picotti P, Iber D, Allain FH
  TITLE     Systems NMR: single-sample quantification of RNA, proteins and 
            metabolites for biomolecular network analysis.
  JOURNAL   Nat Methods. 2019 Aug;16(8):743-749. doi: 10.1038/s41592-019-0495-7.
            Epub 2019 Jul 29.
  PUBMED    31363225
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-019-0495-7"; journalName: "Nat Methods"; date: 
            "2019-08"; volume: "16"; issue: "8"; pages: "743-749"
COMMENT     Sequence Label: pTX1-RNA0
FEATURES             Location/Qualifiers
     source          1..2361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(91..109)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      105..152
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(185..202)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(356..944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(436..455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1115..1906,1907..1975))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1738..1757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1976..2080)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2148..2166
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2204..2226)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2326..2345
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agatcttaat acgactcact atagcaccac acgtgagacc ggatccggct gctaacaaag
       61 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      121 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccgaattcg
      181 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      241 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      301 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      361 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      421 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      481 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      541 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      601 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      661 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      721 ggattagcag agcgaggtat ataggcggtg ctacagagtt cttgaagtgg tggcctaact
      781 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      841 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      901 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      961 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1021 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1081 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1141 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1201 taactacgat acgggagggc ttaccatctg gccccagtgc agcaatgata ccgcgggaac
     1261 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1321 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1381 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     1441 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1501 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1561 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1621 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1681 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     1741 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1801 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1861 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1921 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1981 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     2041 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2101 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2161 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2221 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2281 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     2341 ttgtactgag agtgcaccat a
//
