LOCUS       sequence-246193-        2379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In-vitro-transcription of SMN2-exon7-ESE1 RNA in NMR tube for 
            ""Systems NMR"" analysis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Nikolaev Y, Ripin N, Soste M, Picotti P, Iber D, Allain FH
  TITLE     Systems NMR: single-sample quantification of RNA, proteins and 
            metabolites for biomolecular network analysis.
  JOURNAL   Nat Methods. 2019 Aug;16(8):743-749. doi: 10.1038/s41592-019-0495-7.
            Epub 2019 Jul 29.
  PUBMED    31363225
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-019-0495-7"; journalName: "Nat Methods"; date: 
            "2019-08"; volume: "16"; issue: "8"; pages: "743-749"
COMMENT     Sequence Label: pTX1-SMN2-exon7-ESE1
FEATURES             Location/Qualifiers
     source          1..2379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(109..127)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      123..170
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(203..220)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(374..962)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(454..473)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1133..1924,1925..1993))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1756..1775
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1994..2098)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2166..2184
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2222..2244)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2344..2363
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agatcttaat acgactcact atagcaccac acgggtttta gacaaaatcc gtgagaccgg
       61 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      121 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      181 gaactatatc cgaattcgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      241 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      301 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      361 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      421 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      481 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      541 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      601 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      661 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      721 cagcagccac tggtaacagg attagcagag cgaggtatat aggcggtgct acagagttct
      781 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      841 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      901 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      961 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1021 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1081 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1141 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1201 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgcag
     1261 caatgatacc gcgggaacca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1321 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1381 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1441 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1501 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1561 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1621 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1681 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1741 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1801 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1861 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1921 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1981 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2041 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2101 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     2161 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     2221 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     2281 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     2341 atgcggcatc agagcagatt gtactgagag tgcaccata
//
