LOCUS       sequence-246195-       15223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15223)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX001
FEATURES             Location/Qualifiers
     source          1..15223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5185..5196
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5337..5361)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5390..5479
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5513..5765
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6194..6446)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6914..7939)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6914..7939)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7949..8293)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8435..8452)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8435..8451)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8444..8466)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8591..8610)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8801..8822
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9069..9093
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10689..11837)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12136..12930)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13122..13889
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14582..15213
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggcctc
     4621 aatttcttcg agtcctcgta ctgtagaaga gatcttcaaa gattacaacg ctcgtagaac
     4681 tggcatagtt cgtgctttaa cttatgatgt ggatgaattc tacaatctgt gtgatccaga
     4741 gaaggaaaat ttgtgtctgt atggacatcc aaatgaaact tgggaagtca accttcctgc
     4801 cgaggaagta ccacctgagc tcccagagcc agcacttggg ataaattttg ctagggatgg
     4861 aatgaatcgt agagattggc tttccctcgt tgctgttcac agtgattgtt ggttgctttc
     4921 tgtgtctttc tattttggag ctcgccttaa ccgaaatgaa aggaagcgtc tattcagcat
     4981 gatgaatgat ctgcccaccg tctttgaagt tgtgacagaa agaaagcctc taaaagataa
     5041 gcccagtgct gatagtggga gcagatcacg tggtagcata aagagatcca atgatggtca
     5101 agctaaaagc accccaaggc tggcagatga aagttacgag gaggatgaag aagaacatgg
     5161 ggaaacactc tgtggaagct gtggtgggaa ctacagcgct gatgaatttt ggattggctg
     5221 tgacatctgt gagaagtggt accatggaaa gtgtgtgaag ataacacctg ctaaagctga
     5281 gagtataaaa caatacaaat gcccgtcttg tatgaagcgg gcaagacagc ccggggaccc
     5341 agctttcttg tacaaagtgg ttgataacag cgggttaatt aacatctttt acccatacga
     5401 tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat cctatccata
     5461 tgacgttcca gattacgctg ctcagtgcag cgcttagagc tcgaatttcc ccgatcgttc
     5521 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     5581 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     5641 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     5701 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     5761 agatcgggaa ttagcttcat caacgcaaga catgcgcacg accgtctgac aggagaggaa
     5821 tttccgacga gcacagaaag gacttgctct tggacgtagg cctatttctc aggcacatgt
     5881 atcaagtgtt cggacgtggg ttttcgatgg tgtatcagcc gccgccaact gggagatgag
     5941 gaggctttct tggggggcag tcagcagttc atttcacaag acagaggaac ttgtaaggag
     6001 atgcactgat ttatcttggc gcaaaccagc aggacgaatt agtgggaata gcccgcgaat
     6061 atctaagtta tgcctgtcgg catgagcaga aacttccaat tcgaaacagt ttggagaggt
     6121 tgtttttggg catacctttt gttagtcagc ctctcgattg ctcatcgtca ttacacagta
     6181 ccgaagtttg atcgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
     6241 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
     6301 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
     6361 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
     6421 ctttattgcc aaatgtttga acgatctgct tcgacgcact ccttctttac tccaccatct
     6481 cgtccttatt gaaaacgtgg gtagcaccaa aacgaatcaa gtcgctggaa ctgaagttac
     6541 caatcacgct ggatgatttg ccagttggat taatcttgcc tttccccgca tgaataatat
     6601 tgatgaatgc atgcgtgagg ggtatttcga ttttggcaat agctgcaatt gccgcgacat
     6661 cctccaacga gcataattct tcagaaaaat agcgatgttc catgttgtca gggcatgcat
     6721 gatgcacgtt atgaggtgac ggtgctaggc agtattccct caaagtttca tagtcagtat
     6781 catattcatc attgcattcc tgcaagagag aattgagacg caatccacac gctgcggcaa
     6841 ccttccggcg ttcgtggtct atttgctctt ggacgttgca aacgtaagtg ttggatcccg
     6901 gtcggcatct actctattcc tttgccctcg gacgagtgct ggggcgtcgg tttccactat
     6961 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg
     7021 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag
     7081 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
     7141 gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
     7201 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt
     7261 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt
     7321 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt
     7381 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
     7441 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
     7501 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
     7561 gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt
     7621 cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca
     7681 ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
     7741 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
     7801 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
     7861 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
     7921 tgagttcagg ctttttcata tcggggtcgt cctctccaaa tgaaatgaac ttccttatat
     7981 agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat
     8041 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
     8101 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
     8161 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
     8221 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
     8281 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
     8341 gacgagagtg tcgtgctcca ccatgttgac ggatctctag gacgcgtcga cgcgtcgacg
     8401 cgtcgacgcg tcgacgcgtc ctagaagcta attcactggc cgtcgtttta caacgtcgtg
     8461 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     8521 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     8581 atggcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     8641 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8701 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8761 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8821 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     8881 tcggaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
     8941 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga
     9001 aaagaaaaac caccccagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     9061 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     9121 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     9181 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     9241 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     9301 aacgatgaca gagcgttgct gcctgtgatc aaatatcatc tccctcgcag agatccgaat
     9361 tatcagcctt cttattcatt tctcgcttaa ccgtgacagg ctgtcgatct tgagaactat
     9421 gccgacataa taggaaatcg ctggataaag ccgctgagga agctgagtgg cgctatttct
     9481 ttagaagtga acgttgacga tatcaactcc cctatccatt gctcaccgaa tggtacaggt
     9541 cggggacccg aagttccgac tgtcggcctg atgcatcccc ggctgatcga ccccagatct
     9601 ggggctgaga aagcccagta aggaaacaac tgtaggttcg agtcgcgaga tcccccggaa
     9661 ccaaaggaag taggttaaac ccgctccgat caggccgagc cacgccaggc cgagaacatt
     9721 ggttcctgta ggcatcggga ttggcggatc aaacactaaa gctactggaa cgagcagaag
     9781 tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga ggcacgggag gttgccactt
     9841 gcgggtcagc acggttccga acgccatgga aaccgccccc gccaggcccg ctgcgacgcc
     9901 gacaggatct agcgctgcgt ttggtgtcaa caccaacagc gccacgcccg cagttccgca
     9961 aatagccccc aggaccgcca tcaatcgtat cgggctacct agcagagcgg cagagatgaa
    10021 cacgaccatc agcggctgca cagcgcctac cgtcgccgcg accccgcccg gcaggcggta
    10081 gaccgaaata aacaacaagc tccagaatag cgaaatatta agtgcgccga ggatgaagat
    10141 gcgcatccac cagattcccg ttggaatctg tcggacgatc atcacgagca ataaacccgc
    10201 cggcaacgcc cgcagcagca taccggcgac ccctcggcct cgctgttcgg gctccacgaa
    10261 aacgccggac agatgcgcct tgtgagcgtc cttggggccg tcctcctgtt tgaagaccga
    10321 cagcccaatg atctcgccgt cgatgtaggc gccgaatgcc acggcatctc gcaaccgttc
    10381 agcgaacgcc tccatgggct ttttctcctc gtgctcgtaa acggacccga acatctctgg
    10441 agctttcttc agggccgaca atcggatctc gcggaaatcc tgcacgtcgg ccgctccaag
    10501 ccgtcgaatc tgagccttaa tcacaattgt caattttaat cctctgttta tcggcagttc
    10561 gtagagcgcg ccgtgcgtcc cgagcgatac tgagcgaagc aagtgcgtcg agcagtgccc
    10621 gcttgttcct gaaatgccag taaagcgctg gctgctgaac ccccagccgg aactgacccc
    10681 acaaggccct agcgtttgca atgcaccagg tcatcattga cccaggcgtg ttccaccagg
    10741 ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg
    10801 gtggaatccg atccgcacat gaggcggaag gtttccagct tgagcgggta cggctcccgg
    10861 tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg gtacttctcc
    10921 catatgaatt tcgtgtagtg gtcgccagca aacagcacga cgatttcctc gtcgatcagg
    10981 acctggcaac gggacgtttt cttgccacgg tccaggacgc ggaagcggtg cagcagcgac
    11041 accgattcca ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc
    11101 gacaggcatt cctcggcctt cgtgtaatac cggccattga tcgaccagcc caggtcctgg
    11161 caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc
    11221 aacacctgct gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg
    11281 atcttcacgt ccttgttgac gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt
    11341 ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat cgctcgcatc
    11401 gtgtccggcc acggcgcaat atcgaacaag gaaagctgca tttccttgat ctgctgcttc
    11461 gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct gttttgccag gtcctcgccg
    11521 gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc
    11581 aaacctgccg cctcctgttc gagacgacgc gaacgctcca cggcggccga tggcgcgggc
    11641 agggcagggg gagccagttg cacgctgtcg cgctcgatct tggccgtagc ttgctggacc
    11701 atcgagccga cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg
    11761 gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg
    11821 tcaaacgtcc gattcattca ccctccttgc gggattgccc cgactcacgc cggggcaatg
    11881 tgcccttatt cctgatttga cccgcctggt gccttggtgt ccagataatc caccttatcg
    11941 gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct cgtacttggt attccgaatc
    12001 ttgccctgca cgaataccag cgaccccttg cccaaatact tgccgtgggc ctcggcctga
    12061 gagccaaaac acttgatgcg gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg
    12121 cgccacatct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca
    12181 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
    12241 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
    12301 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
    12361 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
    12421 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
    12481 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
    12541 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
    12601 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
    12661 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
    12721 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
    12781 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
    12841 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
    12901 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
    12961 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
    13021 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
    13081 aacatagtat cgacggagcc gattttgaaa ccacaattat gggtgatgct gccaacttac
    13141 tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt
    13201 ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag
    13261 cgctatctct gctctcactg ccgtaaaaca tggcaactgc agttcactta caccgcttct
    13321 caacccggta cgcaccagaa aatcattgat atggccatga atggcgttgg atgccgggca
    13381 acagcccgca ttatgggcgt tggcctcaac acgattttac gtcacttaaa aaactcaggc
    13441 cgcagtcggt aacctcgcgc atacagccgg gcagtgacgt catcgtctgc gcggaaatgg
    13501 acgaacagtg gggctatgtc ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg
    13561 acagtctccg gaagacggtt gttgcgcacg tattcggtga acgcactatg gcgacgctgg
    13621 ggcgtcttat gagcctgctg tcaccctttg acgtggtgat atggatgacg gatggctggc
    13681 cgctgtatga atcccgcctg aagggaaagc tgcacgtaat cagcaagcga tatacgcagc
    13741 gaattgagcg gcataacctg aatctgaggc agcacctggc acggctggga cggaagtcgc
    13801 tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat ctgaacataa
    13861 aacactatca ataagttgga gtcattaccc aattatgata gaatttacaa gctataaggt
    13921 tattgtcctg ggtttcaagc attagtccat gcaagttttt atgctttgcc cattctatag
    13981 atatattgat aagcgcgctg cctatgcctt gccccctgaa atccttacat acggcgatat
    14041 cttctatata aaagatatat tatcttatca gtattgtcaa tatattcaag gcaatctgcc
    14101 tcctcatcct cttcatcctc ttcgtcttgg tagcttttta aatatggcgc ttcatagagt
    14161 aattctgtaa aggtccaatt ctcgttttca tacctcggta taatcttacc tatcacctca
    14221 aatggttcgc tgggtttatc gcacccccga acacgagcac ggcacccgcg accactatgc
    14281 caagaatgcc caaggtaaaa attgccggcc ccgccatgaa gtccgtgaat gccccgacgg
    14341 ccgaagtgaa gggcaggccg ccacccaggc cgccgccctc actgcccggc acctggtcgc
    14401 tgaatgtcga tgccagcacc tgcggcacgt caatgcttcc gggcgtcgcg ctcgggctga
    14461 tcgcccatcc cgttactgcc ccgatcccgg caatggcaag gactgccagc gctgccattt
    14521 ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg aggcccgcgt
    14581 tagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg cggtcacgcg
    14641 cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa agcaggttaa
    14701 aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct ggattttctg
    14761 cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc actctgcccc
    14821 tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac
    14881 cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag
    14941 gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc
    15001 ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac gtccgcccct
    15061 catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg gccgcggtgt
    15121 ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac ggccgccagc
    15181 ccagcggcga gggcaaccag cccggtgagc gtcggaaagg cgc
//
