LOCUS       sequence-246197-       16030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16030)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX003
FEATURES             Location/Qualifiers
     source          1..16030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6144..6168)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6197..6286
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6320..6572
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7001..7253)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7721..8746)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7721..8746)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8756..9100)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9242..9259)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9242..9258)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9251..9273)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9398..9417)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9608..9629
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9876..9900
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11496..12644)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12943..13737)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13929..14696
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15389..16020
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg tggaatctaa acgactctcc
     4621 acataattta aggggcgatg aatcagaaga aaatgatcac gagagaggta aaggtgttgg
     4681 atccgtttct aattcaagtt catcagcagt ggataataat attgaggaat atggttccga
     4741 ggaagaagaa gataatgaac aaaaaggtaa gaaaaagaga gcatctaatc ctagtaaaat
     4801 ctttggtttc tgtatgatag ctccgggtaa cgacgacaac ttgtcatcgg agagtgcacc
     4861 gccggttacc cggcagtttt ttccggttga tgagttggaa attggagctg ttgccgattc
     4921 tgatgataga tcctctagat ttccaatggc ccattgggct ggagtaaaat tttatcattc
     4981 ggagacatct ggtaatttgc cactgggaaa gggcactgag ttgtcgcagc aagtgcagcc
     5041 tatgaaaaag agccggcgtg gtccaaggtc taggagttca cagtatcgtg gtgttacctt
     5101 ttaccggaga actggccggt gggagtcaca catatgggat tgcgggaagc aagtttattt
     5161 aggcggattt gacacagcac atgcagcagc tcgtgcatat gatagggcag caattaagtt
     5221 ccggggagtg gaggcggaca taaactttaa cctggaggat tatgaggatg acttaaaaca
     5281 gatgaagaat ctgactaagg aagaatttgt gcatgtacta cggagacaaa gtacaggttt
     5341 tccgagggga agttccaagt atagaggggt gactttgcac aaatgtggta gatgggaagc
     5401 tagaatggga cagttattgg gcaaaaagta cgtttatttg ggactctttg atactgaagt
     5461 tgaagctgcc agggcttatg ataaagctgc catcaagtgt aatgggaagg atgcagttac
     5521 gaacttcgat cttagcattt atgaaaatga acttaattca tctgatcaca gtcttgactt
     5581 aagcttgggt ggcgcgagct caaagcaaag cagccgagaa atggaggata ataataatag
     5641 ggataaaagt tcttcaatgc aatttgaagc tgattggagg caccacggtt tgaggcccaa
     5701 gcatcaaact agtccaattg atgttgatgc ccgaagaaga gatggataca atgagtcgga
     5761 aaccttgcag ctcttgagca aaacgcacct acattctcct gcctccttca acccaaacaa
     5821 caatgaaatg caaaggtttg accaatatgt gagagctggt gaatcccaaa tgattcaaat
     5881 gacgtttcca ccacagttca actcgttaaa ttatcacatt caatttccaa gcagcagcaa
     5941 tgggggccga attggagctg caaatagaag agaggtttcg ttttcgtcaa gtgatggtca
     6001 acaatggcaa tccaataatg tccctcatcc tcagcaactt tcaactgctg cagcatcatc
     6061 aggatttcct cagcagatat tcagacatca aaactggtct cacaaaaatg gctgtcatta
     6121 ttctctcatg agacctcccg gggacccagc tttcttgtac aaagtggttg ataacagcgg
     6181 gttaattaac atcttttacc catacgatgt tcctgactat gcgggctatc cctatgacgt
     6241 cccggactat gcaggatcct atccatatga cgttccagat tacgctgctc agtgcagcgc
     6301 ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     6361 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     6421 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     6481 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     6541 atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta gcttcatcaa cgcaagacat
     6601 gcgcacgacc gtctgacagg agaggaattt ccgacgagca cagaaaggac ttgctcttgg
     6661 acgtaggcct atttctcagg cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt
     6721 atcagccgcc gccaactggg agatgaggag gctttcttgg ggggcagtca gcagttcatt
     6781 tcacaagaca gaggaacttg taaggagatg cactgattta tcttggcgca aaccagcagg
     6841 acgaattagt gggaatagcc cgcgaatatc taagttatgc ctgtcggcat gagcagaaac
     6901 ttccaattcg aaacagtttg gagaggttgt ttttgggcat accttttgtt agtcagcctc
     6961 tcgattgctc atcgtcatta cacagtaccg aagtttgatc gatctagtaa catagatgac
     7021 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
     7081 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
     7141 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
     7201 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttcg
     7261 acgcactcct tctttactcc accatctcgt ccttattgaa aacgtgggta gcaccaaaac
     7321 gaatcaagtc gctggaactg aagttaccaa tcacgctgga tgatttgcca gttggattaa
     7381 tcttgccttt ccccgcatga ataatattga tgaatgcatg cgtgaggggt atttcgattt
     7441 tggcaatagc tgcaattgcc gcgacatcct ccaacgagca taattcttca gaaaaatagc
     7501 gatgttccat gttgtcaggg catgcatgat gcacgttatg aggtgacggt gctaggcagt
     7561 attccctcaa agtttcatag tcagtatcat attcatcatt gcattcctgc aagagagaat
     7621 tgagacgcaa tccacacgct gcggcaacct tccggcgttc gtggtctatt tgctcttgga
     7681 cgttgcaaac gtaagtgttg gatcccggtc ggcatctact ctattccttt gccctcggac
     7741 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     7801 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     7861 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     7921 gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa
     7981 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     8041 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     8101 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     8161 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     8221 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     8281 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     8341 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg
     8401 cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     8461 gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt
     8521 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     8581 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     8641 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     8701 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct
     8761 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     8821 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     8881 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     8941 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     9001 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     9061 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     9121 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttgacgga
     9181 tctctaggac gcgtcgacgc gtcgacgcgt cgacgcgtcg acgcgtccta gaagctaatt
     9241 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     9301 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     9361 gcccttccca acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc
     9421 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     9481 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     9541 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     9601 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     9661 tttgatttat aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc
     9721 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     9781 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc
     9841 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     9901 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     9961 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
    10021 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
    10081 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa
    10141 tatcatctcc ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg
    10201 tgacaggctg tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg
    10261 ctgaggaagc tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct
    10321 atccattgct caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg
    10381 catccccggc tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt
    10441 aggttcgagt cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag
    10501 gccgagccac gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa
    10561 cactaaagct actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt
    10621 gagcagaggc acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac
    10681 cgcccccgcc aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac
    10741 caacagcgcc acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg
    10801 gctacctagc agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt
    10861 cgccgcgacc ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga
    10921 aatattaagt gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg
    10981 gacgatcatc acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc
    11041 tcggcctcgc tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt
    11101 ggggccgtcc tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc
    11161 gaatgccacg gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg
    11221 ctcgtaaacg gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg
    11281 gaaatcctgc acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa
    11341 ttttaatcct ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga
    11401 gcgaagcaag tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct
    11461 gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca
    11521 tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg
    11581 acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt
    11641 tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc
    11701 gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac
    11761 agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc
    11821 aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg
    11881 aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg
    11941 ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg
    12001 ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg
    12061 gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc
    12121 ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg
    12181 gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa
    12241 agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg
    12301 ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct
    12361 cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa
    12421 cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc
    12481 tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc
    12541 gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg
    12601 atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg
    12661 attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc
    12721 ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg
    12781 tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc
    12841 aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg
    12901 cgctcctgct tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag
    12961 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
    13021 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
    13081 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
    13141 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
    13201 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
    13261 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
    13321 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
    13381 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
    13441 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
    13501 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    13561 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    13621 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    13681 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    13741 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    13801 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    13861 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca
    13921 caattatggg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
    13981 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
    14041 aacggcaaaa gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg
    14101 caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg
    14161 gccatgaatg gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg
    14221 attttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca
    14281 gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc
    14341 gccagcgctg gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat
    14401 tcggtgaacg cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg
    14461 tggtgatatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc
    14521 acgtaatcag caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc
    14581 acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca
    14641 aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc attacccaat
    14701 tatgatagaa tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca
    14761 agtttttatg ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc
    14821 ccctgaaatc cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta
    14881 ttgtcaatat attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag
    14941 ctttttaaat atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac
    15001 ctcggtataa tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca
    15061 cgagcacggc acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg
    15121 ccatgaagtc cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc
    15181 cgccctcact gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa
    15241 tgcttccggg cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa
    15301 tggcaaggac tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca
    15361 gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca
    15421 ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt
    15481 ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg
    15541 aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc
    15601 gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca
    15661 gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc
    15721 atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc
    15781 cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg
    15841 gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg
    15901 tatccacaac gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg
    15961 tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc
    16021 ggaaaggcgc
//
