LOCUS       sequence-246198-       15344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15344)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX004
FEATURES             Location/Qualifiers
     source          1..15344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5490..5514)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5543..5632
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5666..5918
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6347..6599)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7067..8092)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7067..8092)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8102..8446)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8556..8573)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8556..8572)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8565..8587)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8712..8731)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8922..8943
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9190..9214
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10810..11958)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12257..13051)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13243..14010
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14703..15334
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggctat
     4621 gaaagaaaag ctaaatggcg gtttgaaagg aggaaatgtt aaaactaacg gagtgaaaga
     4681 ggttcactac agaggagtaa gaaagaggcc atggggacgt tacgctgctg aaatccgtga
     4741 tcccagcaag aagagcaggg tttggttagg caccttcgac acggcggaag aggcggcaag
     4801 ggcttatgac gtcgccgcca gagcgttccg aggacccaaa gccaagacga acttcccgtt
     4861 gccgtcggag aatcagtgtc ccagtaacag cagtgaacca acagagtcct ccagtggaga
     4921 aaccggcgtt cacgctcctc acgcgccgtc tgagctcgag ctcacgcgcc cccttggaac
     4981 cgtcgctgta ggctgtgacc atggtggttg taaatccgaa gttggattgg tgagaaatgg
     5041 gtttccgatt tttcatcatc aaccggttat gggagctctg actagtggca gcagcagccc
     5101 tgtagagtca tctagtgggg aaaccggcgt tcacgcgcct cttgagctcg acctcacgcg
     5161 ccatctcggc gccgttggtg agggtggcaa ccatgtccga aatgggtttc ccgtttttta
     5221 tcagcaaccg acagtggcgg ttctgcctaa tggccagcca attttgttgt tcgacagtaa
     5281 ttttgtgcca actggagtac tgaaccggcc aatgccgtac aggtttgaac aggtgacggt
     5341 tgagtttaac ggcgttagtg gtagggtagt tcatagtgac tcgagctcat cgacgacggt
     5401 taacgagaac tattacggag gcggcggcgt atctagaaga gaactaaatc ttgatcttaa
     5461 cctttcccca ccaatggaag ctcccgggga cccagctttc ttgtacaaag tggttgataa
     5521 cagcgggtta attaacatct tttacccata cgatgttcct gactatgcgg gctatcccta
     5581 tgacgtcccg gactatgcag gatcctatcc atatgacgtt ccagattacg ctgctcagtg
     5641 cagcgcttag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
     5701 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     5761 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     5821 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     5881 taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattagctt catcaacgca
     5941 agacatgcgc acgaccgtct gacaggagag gaatttccga cgagcacaga aaggacttgc
     6001 tcttggacgt aggcctattt ctcaggcaca tgtatcaagt gttcggacgt gggttttcga
     6061 tggtgtatca gccgccgcca actgggagat gaggaggctt tcttgggggg cagtcagcag
     6121 ttcatttcac aagacagagg aacttgtaag gagatgcact gatttatctt ggcgcaaacc
     6181 agcaggacga attagtggga atagcccgcg aatatctaag ttatgcctgt cggcatgagc
     6241 agaaacttcc aattcgaaac agtttggaga ggttgttttt gggcatacct tttgttagtc
     6301 agcctctcga ttgctcatcg tcattacaca gtaccgaagt ttgatcgatc tagtaacata
     6361 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
     6421 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
     6481 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
     6541 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
     6601 gcttcgacgc actccttctt tactccacca tctcgtcctt attgaaaacg tgggtagcac
     6661 caaaacgaat caagtcgctg gaactgaagt taccaatcac gctggatgat ttgccagttg
     6721 gattaatctt gcctttcccc gcatgaataa tattgatgaa tgcatgcgtg aggggtattt
     6781 cgattttggc aatagctgca attgccgcga catcctccaa cgagcataat tcttcagaaa
     6841 aatagcgatg ttccatgttg tcagggcatg catgatgcac gttatgaggt gacggtgcta
     6901 ggcagtattc cctcaaagtt tcatagtcag tatcatattc atcattgcat tcctgcaaga
     6961 gagaattgag acgcaatcca cacgctgcgg caaccttccg gcgttcgtgg tctatttgct
     7021 cttggacgtt gcaaacgtaa gtgttggatc ccggtcggca tctactctat tcctttgccc
     7081 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
     7141 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
     7201 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
     7261 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc
     7321 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
     7381 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
     7441 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
     7501 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
     7561 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
     7621 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
     7681 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg
     7741 cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
     7801 caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa
     7861 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     7921 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     7981 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
     8041 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atatcggggt
     8101 cgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
     8161 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
     8221 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     8281 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     8341 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     8401 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     8461 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     8521 gacggatctc taggacgcgt cctagaagct aattcactgg ccgtcgtttt acaacgtcgt
     8581 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     8641 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     8701 aatggcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     8761 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     8821 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     8881 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     8941 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     9001 ttcggaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg
     9061 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg
     9121 aaaagaaaaa ccaccccagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
     9181 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
     9241 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
     9301 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
     9361 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
     9421 taacgatgac agagcgttgc tgcctgtgat caaatatcat ctccctcgca gagatccgaa
     9481 ttatcagcct tcttattcat ttctcgctta accgtgacag gctgtcgatc ttgagaacta
     9541 tgccgacata ataggaaatc gctggataaa gccgctgagg aagctgagtg gcgctatttc
     9601 tttagaagtg aacgttgacg atatcaactc ccctatccat tgctcaccga atggtacagg
     9661 tcggggaccc gaagttccga ctgtcggcct gatgcatccc cggctgatcg accccagatc
     9721 tggggctgag aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga
     9781 accaaaggaa gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat
     9841 tggttcctgt aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa
     9901 gtcctccggc cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact
     9961 tgcgggtcag cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc
    10021 cgacaggatc tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc
    10081 aaatagcccc caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga
    10141 acacgaccat cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt
    10201 agaccgaaat aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga
    10261 tgcgcatcca ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg
    10321 ccggcaacgc ccgcagcagc ataccggcga cccctcggcc tcgctgttcg ggctccacga
    10381 aaacgccgga cagatgcgcc ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg
    10441 acagcccaat gatctcgccg tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt
    10501 cagcgaacgc ctccatgggc tttttctcct cgtgctcgta aacggacccg aacatctctg
    10561 gagctttctt cagggccgac aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa
    10621 gccgtcgaat ctgagcctta atcacaattg tcaattttaa tcctctgttt atcggcagtt
    10681 cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc
    10741 cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg gaactgaccc
    10801 cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag
    10861 gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg
    10921 ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg
    10981 gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc
    11041 ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag
    11101 gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga
    11161 caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg
    11221 cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg
    11281 gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc
    11341 caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt
    11401 gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat
    11461 tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat
    11521 cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt
    11581 cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc
    11641 ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc
    11701 caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg
    11761 cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac
    11821 catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat
    11881 ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg
    11941 gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat
    12001 gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc
    12061 ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat
    12121 cttgccctgc acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg
    12181 agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt
    12241 gcgccacatc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
    12301 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
    12361 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
    12421 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
    12481 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
    12541 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
    12601 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
    12661 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
    12721 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
    12781 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
    12841 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
    12901 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
    12961 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
    13021 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
    13081 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
    13141 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
    13201 taacatagta tcgacggagc cgattttgaa accacaatta tgggtgatgc tgccaactta
    13261 ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg
    13321 tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca
    13381 gcgctatctc tgctctcact gccgtaaaac atggcaactg cagttcactt acaccgcttc
    13441 tcaacccggt acgcaccaga aaatcattga tatggccatg aatggcgttg gatgccgggc
    13501 aacagcccgc attatgggcg ttggcctcaa cacgatttta cgtcacttaa aaaactcagg
    13561 ccgcagtcgg taacctcgcg catacagccg ggcagtgacg tcatcgtctg cgcggaaatg
    13621 gacgaacagt ggggctatgt cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat
    13681 gacagtctcc ggaagacggt tgttgcgcac gtattcggtg aacgcactat ggcgacgctg
    13741 gggcgtctta tgagcctgct gtcacccttt gacgtggtga tatggatgac ggatggctgg
    13801 ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag
    13861 cgaattgagc ggcataacct gaatctgagg cagcacctgg cacggctggg acggaagtcg
    13921 ctgtcgttct caaaatcggt ggagctgcat gacaaagtca tcgggcatta tctgaacata
    13981 aaacactatc aataagttgg agtcattacc caattatgat agaatttaca agctataagg
    14041 ttattgtcct gggtttcaag cattagtcca tgcaagtttt tatgctttgc ccattctata
    14101 gatatattga taagcgcgct gcctatgcct tgccccctga aatccttaca tacggcgata
    14161 tcttctatat aaaagatata ttatcttatc agtattgtca atatattcaa ggcaatctgc
    14221 ctcctcatcc tcttcatcct cttcgtcttg gtagcttttt aaatatggcg cttcatagag
    14281 taattctgta aaggtccaat tctcgttttc atacctcggt ataatcttac ctatcacctc
    14341 aaatggttcg ctgggtttat cgcacccccg aacacgagca cggcacccgc gaccactatg
    14401 ccaagaatgc ccaaggtaaa aattgccggc cccgccatga agtccgtgaa tgccccgacg
    14461 gccgaagtga agggcaggcc gccacccagg ccgccgccct cactgcccgg cacctggtcg
    14521 ctgaatgtcg atgccagcac ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg
    14581 atcgcccatc ccgttactgc cccgatcccg gcaatggcaa ggactgccag cgctgccatt
    14641 tttggggtga ggccgttcgc ggccgagggg cgcagcccct ggggggatgg gaggcccgcg
    14701 ttagcgggcc gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc
    14761 gcacagggcg cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta
    14821 aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct
    14881 gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc
    14941 ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata
    15001 ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca
    15061 ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg
    15121 cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc
    15181 tcatctgtca gtgagggcca agttttccgc gaggtatcca caacgccggc ggccgcggtg
    15241 tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca gggccataga cggccgccag
    15301 cccagcggcg agggcaacca gcccggtgag cgtcggaaag gcgc
//
