LOCUS       sequence-246200-       11319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. 
            UNG; for direct neuronal conversion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11319)
  AUTHORS   Schafer ST, Paquola ACM, Stern S, Gosselin D, Ku M, Pena M, Kuret 
            TJM, Liyanage M, Mansour AA, Jaeger BN, Marchetto MC, Glass CK, 
            Mertens J, Gage FH
  TITLE     Pathological priming causes developmental gene network 
            heterochronicity in autistic subject-derived neurons.
  JOURNAL   Nat Neurosci. 2019 Feb;22(2):243-255. doi: 
            10.1038/s41593-018-0295-x. Epub 2019 Jan 7.
  PUBMED    30617258
REFERENCE   2  (bases 1 to 11319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-018-0295-x"; journalName: "Nat Neurosci"; date: 
            "2019-02"; volume: "22"; issue: "2"; pages: "243-255"
COMMENT     Sequence Label: pLVX-UbC-rtTA-Ngn2:2A:EGFP
FEATURES             Location/Qualifiers
     source          1..11319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(join(2184..2195,2196..2231,2232..2234,2235..2270,
                     2271..2273,2274..2309,2310..2315,2316..2933))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPGN"
     promoter        complement(2943..4154)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(3087..3106)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        4192..4506
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4200..4218
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4236..4254
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4271..4289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4307..4325
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4343..4361
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4378..4396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4414..4432
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4479..4503
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5359..5415
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5416..5418,5419..5421,5422..6135)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5416..5418,5419..5421,5422..6132)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5461..5482)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5722..5741)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6069..6090
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6152..6651
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6187..6210)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6584..6603
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6672..7271
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6672..6691)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7168..7188
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7288..7876
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7341..7361)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7759..7770)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8083..8716
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8844..8860)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8844..8860)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8857..8879)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8868..8884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8892..8898,8899..8916,8917..8922))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8937..8958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9075..9092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9246..9834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9326..9345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10005..10796,10797..10865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10628..10647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10866..10970)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11018..11152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11055..11074)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11109..11128
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgattagtta cccgggagca tgtcaaggtc aaaatcgtca
     2221 agagcgtcag caggcagcat atcaaggtca aagtcgtcaa gggcatcggc tgggagcatg
     2281 tctaagtcaa aatcgtcaag ggcgtcggcc ggcccgccgc tttcgcactt tagctgtttc
     2341 tccaggccac atatgattag ttccaggccg aaaaggaagg caggttcggc tccctgccgg
     2401 tcgaacagct caattgcttg tctcagaagt gggggcatag aatcggtggt aggtgtctct
     2461 ctttcctctt ttgctacttg atgctcctgt tcctccaata cgcagcccag tgtaaagtgg
     2521 cccacggcgg acagagcgta cagtgcgttc tccagggaga agccttgctg acacaggaac
     2581 gcgagctgat tttccagggt ttcgtactgt ttctctgttg ggcgggtgcc gagatgcact
     2641 ttagccccgt cgcgatgtga gaggagagca cagcggaatg acttggcgtt gttccgcaga
     2701 aagtcttgcc atgactcgcc ttccaggggg cagaagtggg tatgatgcct gtccagcatc
     2761 tcgattggca gggcatcgag cagggcccgc ttgttcttca cgtgccagta cagggtaggc
     2821 tgctcaactc ccagcttttg agcgagtttc cttgtcgtca ggccttcgat accgactcca
     2881 ttgagtaatt ccagagcgcc gtttatgact ttgctcttgt ccagtctaga catggtggat
     2941 ccgtctaaca aaaaagccaa aaacggccag aatttagcgg acaatttact agtctaacac
     3001 tgaaaattac atattgaccc aaatgattac atttcaaaag gtgcctaaaa aacttcacaa
     3061 aacacactcg ccaaccccga gcgcatagtt caaaaccgga gcttcagcta cttaagaaga
     3121 taggtacata aaaccgacca aagaaactga cgcctcactt atccctcccc tcaccagagg
     3181 tccggcgcct gtcgattcag gagagcctac cctaggcccg aaccctgcgt cctgcgacgg
     3241 agaaaagcct accgcacacc taccggcagg tggccccacc ctgcattata agccaacaga
     3301 acgggtgacg tcacgacacg acgagggcgc gcgctcccaa aggtacgggt gcactgccca
     3361 acggcaccgc cataactgcc gcccccgcaa cagacgacaa accgagttct ccagtcagtg
     3421 acaaacttca cgtcagggtc cccagatggt gccccagccc atctcacccg aataagagct
     3481 ttcccgcatt agcgaaggcc tcaagacctt gggttcttgc cgcccaccat gccccccacc
     3541 ttgtttcaac gacctcacag cccgcctcac aagcgtcttc cattcaagac tcgggaacag
     3601 ccgccatttt gctgcgctcc ccccaacccc cagttcaggg caaccttgct cgcggaccca
     3661 gactacagcc cttggcggtc tctccacacg cttccgtccc accgagcggc ccggcggcca
     3721 cgaaagcccc ggccagccca gcagcccgct actcaccaag tgacgatcac agcgatccac
     3781 aaacaagaac cgcgacccaa atcccggctg cgacggaact agctgtgcca cacccggcgc
     3841 gtccttatat aatcatcggc gttcaccgcc ccacggagat ccctccgcag aatcgccgag
     3901 aagggactac ttttcctcgc ctgttccgct ctctggaaag aaaaccagtg ccctagagtc
     3961 acccaagtcc cgtcctaaaa tgtccttctg ctgatactgg ggttctaagg ccgagtctta
     4021 tgagcagcgg gccgctgtcc tgagcgtccg ggcggaagga tcaggacgct cgctgcgccc
     4081 ttcgtctgac gtggcagcgc tcgccgtgag gaggggggcg cccgcgggag gcgccaaaac
     4141 ccggcgcgga ggccagatcg gcgcatcgat gaggcccttt cgtcttcact cgagtttact
     4201 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
     4261 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     4321 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
     4381 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     4441 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
     4501 atcgcctgga gaaggatccg cggccgcgcc gccaccatgt tcgtcaaatc cgagaccttg
     4561 gagttgaagg aggaagagga cgtgttagtg ctgctcggat cggcctcccc cgccttggcg
     4621 gccctgaccc cgctgtcatc cagcgccgac gaagaagagg aggaggagcc gggcgcgtca
     4681 ggcggggcgc gtcggcagcg cggggctgag gccgggcagg gggcgcgggg cggcgtggct
     4741 gcgggtgcgg agggctgccg gcccgcacgg ctgctgggtc tggtacacga ttgcaaacgg
     4801 cgcccttccc gggcgcgggc cgtctcccga ggcgccaaga cggccgagac ggtgcagcgc
     4861 atcaagaaga cccgtagact gaaggccaac aaccgcgagc gaaaccgcat gcacaacctc
     4921 aacgcggcac tggacgcgct gcgcgaggtg ctccccacgt tccccgagga cgccaagctc
     4981 accaagatcg agaccctgcg cttcgcccac aactacatct gggcactcac cgagaccctg
     5041 cgcctggcgg atcactgcgg gggcggcggc gggggcctgc cgggggcgct cttctccgag
     5101 gcagtgttgc tgagcccggg aggcgccagc gccgccctga gcagcagcgg agacagcccc
     5161 tcgcccgcct ccacgtggag ttgcaccaac agccccgcgc cgtcctcctc cgtgtcctcc
     5221 aattccacct ccccctacag ctgcacttta tcgcccgcca gcccggccgg gtcagacatg
     5281 gactattggc agcccccacc tcccgacaag caccgctatg cacctcacct ccccatagcc
     5341 agggattgta tccatatggc taccaacttc tcacttctca agcaggctgg tgacgtcgaa
     5401 gagaatcctg gcccaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     5461 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     5521 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     5581 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     5641 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     5701 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     5761 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     5821 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     5881 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     5941 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     6001 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     6061 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     6121 gagctgtaca agtaaacgcg tgaattctac cgggtagggg aggcgctttt cccaaggcag
     6181 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     6241 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     6301 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     6361 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     6421 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     6481 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     6541 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     6601 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     6661 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6721 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6781 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6841 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6901 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6961 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     7021 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     7081 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     7141 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     7201 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     7261 ccggtgcctg acgggcgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     7321 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7381 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7441 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7501 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7561 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7621 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7681 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7741 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7801 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7861 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     7921 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     7981 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     8041 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     8101 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     8161 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     8221 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     8281 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     8341 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     8401 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     8461 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     8521 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8581 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8641 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8701 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     8761 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct
     8821 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8881 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8941 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9001 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9061 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9121 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9181 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9481 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9661 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9721 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9961 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10021 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10081 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10141 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10201 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10261 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10321 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10381 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10441 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10501 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10561 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10621 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10681 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10741 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10801 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10861 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10921 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10981 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
    11041 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    11101 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    11161 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    11221 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    11281 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
