LOCUS       sequence-24623-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA
  TITLE     Targeted gene inactivation in zebrafish using engineered zinc-finger
            nucleases.
  JOURNAL   Nat Biotechnol. 2008 Jun . 26(6):695-701.
  PUBMED    18500337
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2008 Jun . 26(6):695-701."
COMMENT     Sequence Label: 1352wUV2 F2bbs kan GCANNNAGGk
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             218..487
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     CDS             497..520
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cattcctacg cagcaatgca acctcccaat tcaagtgcgg acaacaaccc tttgcaacag
       61 caatcatcac aaaataccgt accaaacgtc ctcaaccaaa ttaaccaaat cttttctcca
      121 gaggagcaac gcagcttatt acaagaagcc atcgaaacct gcaagaattt tgaaaaaaca
      181 caattgtaag tctcggctcc aagaaggaga tatacccatg gcacgcgtaa ctgttcagga
      241 cgctgtagag aaaattggta accgttttga cctggtactg gtcgccgcgc gtcgcgctcg
      301 tcagatgcag gtaggcggaa aggacccgct cgtaccggaa gaaaacgata aaaccactgt
      361 aatcgcgctg cgcgaaatcg aagaaggtct gatcaacaac cagatcctcg acgttcgcga
      421 acgccaggaa cagcaagagc aggaagccgc tgaattacaa gccgttaccg ctattgctga
      481 aggtcgtgcg gccgcggact acaaggatga cgacgacaag ttccggaccg gttccaagac
      541 acccccccat ggtacccgcc catatgcttg ccctgtcgag tcctgcgacc gccgcttctc
      601 tcggtccgac cacctgaccc agcatatccg gattcacaca ggccagaagc ccttccagtg
      661 tcgcatctgc cattnnggng agngagaanc aaatgncccg ggnggcaccc gagcgttctg
      721 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
      781 ctctcgaacc cagagtccgc tcagaa
//
