LOCUS       sequence-246239-         311 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SV40 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Hazelbaker DZ, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D,
            Eggan K, Barrett LE
  TITLE     A multiplexed gRNA piggyBac transposon system facilitates efficient 
            induction of CRISPRi and CRISPRa in human pluripotent stem cells.
  JOURNAL   Sci Rep. 2020 Jan 20;10(1):635. doi: 10.1038/s41598-020-57500-1.
  PUBMED    31959800
REFERENCE   2  (bases 1 to 311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-01-20"; volume: "10(1):635. doi"; pages: " 
            10.1038/s41598-020-57500-1"
COMMENT     Sequence Label: S6_BstBI_SV40_SwaI
FEATURES             Location/Qualifiers
     source          1..311
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        57..267
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        79..275
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      126..261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ttcgaatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaactc
      121 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
      181 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      241 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt accaccggag
      301 cttatttaaa t
//
