LOCUS       sequence-246245-         514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PGK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Hazelbaker DZ, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D,
            Eggan K, Barrett LE
  TITLE     A multiplexed gRNA piggyBac transposon system facilitates efficient 
            induction of CRISPRi and CRISPRa in human pluripotent stem cells.
  JOURNAL   Sci Rep. 2020 Jan 20;10(1):635. doi: 10.1038/s41598-020-57500-1.
  PUBMED    31959800
REFERENCE   2  (bases 1 to 514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-01-20"; volume: "10(1):635. doi"; pages: " 
            10.1038/s41598-020-57500-1"
COMMENT     Sequence Label: S12_EcoRV_PGK_AscI
FEATURES             Location/Qualifiers
     source          1..514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..506
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(42..65)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     439..458
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gatatcgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
       61 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
      121 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
      181 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
      241 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
      301 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
      361 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
      421 ggtcctcccg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
      481 cctcttcctc atctccgggc ctttcgggcg cgcc
//
