LOCUS       sequence-246246-        1233 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  UBC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Hazelbaker DZ, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D,
            Eggan K, Barrett LE
  TITLE     A multiplexed gRNA piggyBac transposon system facilitates efficient 
            induction of CRISPRi and CRISPRa in human pluripotent stem cells.
  JOURNAL   Sci Rep. 2020 Jan 20;10(1):635. doi: 10.1038/s41598-020-57500-1.
  PUBMED    31959800
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-01-20"; volume: "10(1):635. doi"; pages: " 
            10.1038/s41598-020-57500-1"
COMMENT     Sequence Label: S13_EcoRV_UBC_AscI
FEATURES             Location/Qualifiers
     source          1..1233
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..1221
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1058..1077
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gatatcgatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
       61 ggcgagcgct gccacgtcag acgaagggcg caggagcgtc ctgatccttc cgcccggacg
      121 ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag
      181 gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga
      241 acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt
      301 gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg
      361 ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg
      421 ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg
      481 gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt
      541 ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg
      601 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc
      661 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
      721 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
      781 gggcggcagt tatgcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct
      841 cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg
      901 gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct
      961 ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt
     1021 ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg
     1081 cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca
     1141 tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg
     1201 tttttggctt ttttgttaga cgaagggcgc gcc
//
