LOCUS       sequence-24626-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA
  TITLE     Targeted gene inactivation in zebrafish using engineered zinc-finger
            nucleases.
  JOURNAL   Nat Biotechnol. 2008 Jun . 26(6):695-701.
  PUBMED    18500337
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2008 Jun . 26(6):695-701."
COMMENT     Sequence Label: pCS2-Flag-TTGZFP-FokI-DD
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..46
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             47..112
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ttttttgcgg atctgcccca tggctccaaa gaagaagcgt aaggtagact acaaagacca
       61 tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca agggtacccg
      121 cccatatgct tgccctgtcg agtcctgtga ccgccgcttc agccagaagg ggcacctcac
      181 ccgccatatc cggattcaca caggccagaa gcccttccag tgtcgcatct gcatgaggaa
      241 cttcagccgc agcgacgcgc tcacccgcca catccgcacc cacacaggcg agaagccttt
      301 tgcatgcgac atctgcggca ggaagttcgc gcgcagcgac gcgctcagga agcacaccaa
      361 aatccataca ggtggatccc aactagtcaa aagtgaactg gaggagaaga aatctgaact
      421 tcgtcataaa ttgaaatatg tgcctcatga atatattgaa ttaattgaaa ttgccagaaa
      481 ttccactcag gatagaattc ttgaaatgaa ggtaatggaa ttttttatga aagtttatgg
      541 atatagaggt aaacatttgg gtggatcaag gaaaccggac ggagcaattt atactgtcgg
      601 atctcctngg gnnnnnnggt ntgcgggtga atactaaagc ttatagcgga ggttataatc
      661 tgccattgnc agcgagantg tgatattntn tntntnaaaa aattcaaccg aacnacttnt
      721 ccccctgggg ggggggangt cttcctctnc nnacgaatta nntttttttn gagngncctt
      781 nagga
//
