LOCUS       sequence-246263-       15220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15220)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX009
FEATURES             Location/Qualifiers
     source          1..15220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5334..5358)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5387..5476
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5510..5762
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6191..6443)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6911..7936)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6911..7936)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7946..8290)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8432..8449)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8432..8448)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8441..8463)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8588..8607)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8798..8819
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9066..9090
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10686..11834)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12133..12927)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13119..13886
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14579..15210
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggattc
     4621 ttcttgttct tctcattttt tctatcctat gaattctgat ctttcttctg attcttcttg
     4681 ggaatggtcc actttaaact caactaattc tcttcctttt aaccttaacg attccgaaga
     4741 aatgcttctt tttggtgttc ttactaacgc agctcaagaa acaacgtcgg aaacagttac
     4801 ctcgtaccat gttaaggaag aggaagttag ttcaaaatct aaggttgtaa aagaaattga
     4861 agaaaaaccg gccaaggaga agtcgtttcg cggcgttaga aggcggccgt ggggaaaatt
     4921 cgcggcggag ataagggatt ctactaggaa tggtgttagg gtatggttag ggacatttga
     4981 tagtccagag gcagctgctt tagcgtatga tcaagccgct ttttcaatac ggggtacatc
     5041 agcaatcttg aattttcctg tggaaacagt tcaagaatcg ctacgtgaca tgaaatgcca
     5101 cgtggatgaa gacggttctc ctgtagtggc gctgaagaag cgccactcaa tgaggaagag
     5161 gagcttgaat tccaagaaca acagtaatag taaagttgtg agggaagtaa aaatggagaa
     5221 tgtaaatgtt gtagtatttg aagatttggg ggctgattat ttggaacaac ttttgagtag
     5281 tagttcaagt gatcaaagta gttgtaatga agcttttcta ccatggcccg gggacccagc
     5341 tttcttgtac aaagtggttg ataacagcgg gttaattaac atcttttacc catacgatgt
     5401 tcctgactat gcgggctatc cctatgacgt cccggactat gcaggatcct atccatatga
     5461 cgttccagat tacgctgctc agtgcagcgc ttagagctcg aatttccccg atcgttcaaa
     5521 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     5581 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     5641 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     5701 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     5761 tcgggaatta gcttcatcaa cgcaagacat gcgcacgacc gtctgacagg agaggaattt
     5821 ccgacgagca cagaaaggac ttgctcttgg acgtaggcct atttctcagg cacatgtatc
     5881 aagtgttcgg acgtgggttt tcgatggtgt atcagccgcc gccaactggg agatgaggag
     5941 gctttcttgg ggggcagtca gcagttcatt tcacaagaca gaggaacttg taaggagatg
     6001 cactgattta tcttggcgca aaccagcagg acgaattagt gggaatagcc cgcgaatatc
     6061 taagttatgc ctgtcggcat gagcagaaac ttccaattcg aaacagtttg gagaggttgt
     6121 ttttgggcat accttttgtt agtcagcctc tcgattgctc atcgtcatta cacagtaccg
     6181 aagtttgatc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
     6241 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
     6301 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
     6361 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
     6421 tattgccaaa tgtttgaacg atctgcttcg acgcactcct tctttactcc accatctcgt
     6481 ccttattgaa aacgtgggta gcaccaaaac gaatcaagtc gctggaactg aagttaccaa
     6541 tcacgctgga tgatttgcca gttggattaa tcttgccttt ccccgcatga ataatattga
     6601 tgaatgcatg cgtgaggggt atttcgattt tggcaatagc tgcaattgcc gcgacatcct
     6661 ccaacgagca taattcttca gaaaaatagc gatgttccat gttgtcaggg catgcatgat
     6721 gcacgttatg aggtgacggt gctaggcagt attccctcaa agtttcatag tcagtatcat
     6781 attcatcatt gcattcctgc aagagagaat tgagacgcaa tccacacgct gcggcaacct
     6841 tccggcgttc gtggtctatt tgctcttgga cgttgcaaac gtaagtgttg gatcccggtc
     6901 ggcatctact ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg
     6961 cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac
     7021 gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg
     7081 catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca
     7141 tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg
     7201 tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt
     7261 gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg
     7321 tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct
     7381 cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca
     7441 tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg
     7501 tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat
     7561 cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg
     7621 tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc
     7681 tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt
     7741 gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat
     7801 atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca
     7861 tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga
     7921 gttcaggctt tttcatatcg gggtcgtcct ctccaaatga aatgaacttc cttatataga
     7981 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc
     8041 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     8101 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     8161 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     8221 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     8281 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     8341 gagagtgtcg tgctccacca tgttgacgga tctctaggac gcgtcgacgc gtcgacgcgt
     8401 cgacgcgtcg acgcgtccta gaagctaatt cactggccgt cgttttacaa cgtcgtgact
     8461 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     8521 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     8581 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8641 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8701 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8761 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8821 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     8881 gaaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     8941 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     9001 gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     9061 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     9121 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     9181 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     9241 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     9301 gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc ctcgcagaga tccgaattat
     9361 cagccttctt attcatttct cgcttaaccg tgacaggctg tcgatcttga gaactatgcc
     9421 gacataatag gaaatcgctg gataaagccg ctgaggaagc tgagtggcgc tatttcttta
     9481 gaagtgaacg ttgacgatat caactcccct atccattgct caccgaatgg tacaggtcgg
     9541 ggacccgaag ttccgactgt cggcctgatg catccccggc tgatcgaccc cagatctggg
     9601 gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc cccggaacca
     9661 aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga gaacattggt
     9721 tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga gcagaagtcc
     9781 tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt gccacttgcg
     9841 ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg cgacgccgac
     9901 aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag ttccgcaaat
     9961 agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag agatgaacac
    10021 gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca ggcggtagac
    10081 cgaaataaac aacaagctcc agaatagcga aatattaagt gcgccgagga tgaagatgcg
    10141 catccaccag attcccgttg gaatctgtcg gacgatcatc acgagcaata aacccgccgg
    10201 caacgcccgc agcagcatac cggcgacccc tcggcctcgc tgttcgggct ccacgaaaac
    10261 gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc tcctgtttga agaccgacag
    10321 cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca accgttcagc
    10381 gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca tctctggagc
    10441 tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg ctccaagccg
    10501 tcgaatctga gccttaatca caattgtcaa ttttaatcct ctgtttatcg gcagttcgta
    10561 gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc agtgcccgct
    10621 tgttcctgaa atgccagtaa agcgctggct gctgaacccc cagccggaac tgaccccaca
    10681 aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg
    10741 ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg
    10801 gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc
    10861 gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat
    10921 atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc
    10981 tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc
    11041 gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac
    11101 aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa
    11161 agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac
    11221 acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc
    11281 ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc
    11341 ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg
    11401 tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg
    11461 tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg
    11521 gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa
    11581 cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg
    11641 gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc
    11701 gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt
    11761 tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca
    11821 aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg ggcaatgtgc
    11881 ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac cttatcggca
    11941 atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt ccgaatcttg
    12001 ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc ggcctgagag
    12061 ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc
    12121 cacatctagg tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc
    12181 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc
    12241 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
    12301 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
    12361 gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc
    12421 gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc
    12481 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
    12541 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
    12601 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
    12661 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
    12721 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
    12781 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
    12841 agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa
    12901 ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct acagtattta
    12961 aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc
    13021 taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac
    13081 atagtatcga cggagccgat tttgaaacca caattatggg tgatgctgcc aacttactga
    13141 tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc
    13201 tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc
    13261 tatctctgct ctcactgccg taaaacatgg caactgcagt tcacttacac cgcttctcaa
    13321 cccggtacgc accagaaaat cattgatatg gccatgaatg gcgttggatg ccgggcaaca
    13381 gcccgcatta tgggcgttgg cctcaacacg attttacgtc acttaaaaaa ctcaggccgc
    13441 agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg
    13501 aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg gctgttttac gcgtatgaca
    13561 gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactatggcg acgctggggc
    13621 gtcttatgag cctgctgtca ccctttgacg tggtgatatg gatgacggat ggctggccgc
    13681 tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag caagcgatat acgcagcgaa
    13741 ttgagcggca taacctgaat ctgaggcagc acctggcacg gctgggacgg aagtcgctgt
    13801 cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg gcattatctg aacataaaac
    13861 actatcaata agttggagtc attacccaat tatgatagaa tttacaagct ataaggttat
    13921 tgtcctgggt ttcaagcatt agtccatgca agtttttatg ctttgcccat tctatagata
    13981 tattgataag cgcgctgcct atgccttgcc ccctgaaatc cttacatacg gcgatatctt
    14041 ctatataaaa gatatattat cttatcagta ttgtcaatat attcaaggca atctgcctcc
    14101 tcatcctctt catcctcttc gtcttggtag ctttttaaat atggcgcttc atagagtaat
    14161 tctgtaaagg tccaattctc gttttcatac ctcggtataa tcttacctat cacctcaaat
    14221 ggttcgctgg gtttatcgca cccccgaaca cgagcacggc acccgcgacc actatgccaa
    14281 gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc cgtgaatgcc ccgacggccg
    14341 aagtgaaggg caggccgcca cccaggccgc cgccctcact gcccggcacc tggtcgctga
    14401 atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg cgtcgcgctc gggctgatcg
    14461 cccatcccgt tactgccccg atcccggcaa tggcaaggac tgccagcgct gccatttttg
    14521 gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag
    14581 cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac
    14641 agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag
    14701 acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct
    14761 gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca
    14821 agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc
    14881 agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg
    14941 ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc
    15001 tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat
    15061 ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc
    15121 gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca
    15181 gcggcgaggg caaccagccc ggtgagcgtc ggaaaggcgc
//
