LOCUS       sequence-24627-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA
  TITLE     Targeted gene inactivation in zebrafish using engineered zinc-finger
            nucleases.
  JOURNAL   Nat Biotechnol. 2008 Jun . 26(6):695-701.
  PUBMED    18500337
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2008 Jun . 26(6):695-701."
COMMENT     Sequence Label: 1352wUV2 F3bbs kan NNNTGGGCGk
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             219..488
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     CDS             498..521
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgatncntac gcagcaatgc aacctcccaa ttcaagtgcg gacaacaacc ctttgcaaca
       61 gcaatcatca caaaataccg taccaaacgt cctcaaccaa attaaccaaa tcttttctcc
      121 agaggagcaa cgcagcttat tacaagaagc catcgaaacc tgcaagaatt ttgaaaaaac
      181 acaattgtaa gtctcggctc caagaaggag atatacccat ggcacgcgta actgttcagg
      241 acgctgtaga gaaaattggt aaccgttttg acctggtact ggtcgccgcg cgtcgcgctc
      301 gtcagatgca ggtaggcgga aaggacccgc tcgtaccgga agaaaacgat aaaaccactg
      361 taatcgcgct gcgcgaaatc gaagaaggtc tgatcaacaa ccagatcctc gacgttcgcg
      421 aacgccagga acagcaagag caggaagccg ctgaattaca agccgttacc gctattgctg
      481 aaggtcgtgc ggccgcggac tacaaggatg acgacgacaa gttccggacc ggttccaaga
      541 caccccccca tggtacccgc ccatatgctt gccctgtcga gtcctgcgat cgccgctttt
      601 ctcgctcgga tgagcttacc cgccatatcc gcatccatac cggtcagaag cccttccagt
      661 gtcgaatctg tatgcgtaac ttcagtcnan gnggaccacn tttaccaccc anntcngcac
      721 ccaaanatgg cgagaagcct tttgcatgcg acatctgcgg gtcttctgag tgaggatcca
      781 aacgcccggc ggca
//
