LOCUS       sequence-246288-       12866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expressing nuclear BirA* (R118G)-YFP under the 
            UBQ10 promoter in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12866)
  AUTHORS   Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC
  TITLE     Proximity labeling of protein complexes and cell type-specific 
            organellar proteomes in Arabidopsis enabled by TurboID.
  JOURNAL   Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864.
  PUBMED    31535972
REFERENCE   2  (bases 1 to 12866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-19"; volume: "8. pii: 47864. doi"; pages: " 
            10.7554/eLife.47864"
COMMENT     Sequence Label: R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS
FEATURES             Location/Qualifiers
     source          1..12866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             39..80
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             273..1043
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAIGLSLVIGIVMAEVLRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             join(1071..1073,1074..1076,1077..1787)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1116..1137)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1377..1396)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1724..1745
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1797..1817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1837..1861)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1893..2145
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2178..2194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2178..2194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2191..2213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2202..2218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2226..2232,2233..2250,2251..2256))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2271..2292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2328..2580)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2740..3291)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(3343..3522)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4028..4052
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4573..5364
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5610..6198
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6099..6118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6352..6369
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6384..6524
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6450..6469)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6610..6632
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6868..7062
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7128..8201)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8630..9259)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10560..10584
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10772..10794
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10786..10803
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10787..10803
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10828..10848
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ccaagtttgt acaaaaaagc aggctccgcg gccgcatggg caagcccatc cccaaccccc
       61 tgctgggcct ggacagcacc gctagcaagg ataacaccgt gcccctgaaa ctgattgctc
      121 tgctggctaa tggcgagttc catagtggcg aacagctggg agaaaccctg ggcatgtcca
      181 gggccgctat caacaagcac attcagactc tgcgcgactg gggcgtggac gtgttcaccg
      241 tgcccggaaa gggctactct ctgcccgagc ctatccagct gctgaacgct aaacagattc
      301 tgggacagct ggacggcggg agcgtggcag tcctgcctgt gatcgactcc accaatcagt
      361 acctgctgga tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc
      421 aggcaggaag aggagggaga gggaggaaat ggttctctcc ttttggagct aacctgtacc
      481 tgagtatgtt ttggcgcctg gagcagggac cagcagcaat cggcctgagc ctggtcatcg
      541 gaattgtcat ggcagaagtg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc
      601 ccaatgacct gtatctgcag gatagaaagc tggcaggcat cctggtggag ctgaccggaa
      661 aaacaggcga tgctgcacag atcgtcattg gcgccgggat taacatggct atgaggcgcg
      721 tggaggaaag cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg
      781 acaggaatac tctggccgct atgctgatcc gagagctgcg ggcagccctg gaactgttcg
      841 agcaggaagg cctggctcca tatctgtcac ggtgggagaa gctggataac ttcatcaata
      901 gacccgtgaa gctgatcatt ggggacaaag agattttcgg gattagccgg ggaattgata
      961 aacagggagc cctgctgctg gaacaggacg gaattatcaa accctggatg ggcggagaaa
     1021 tcagtctgcg gtctgccgaa aagggaggcg gtggaagcgg cggaggttcc atggtgagca
     1081 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1141 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1201 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1261 ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     1321 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1381 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1441 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1501 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1561 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1621 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     1681 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1741 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggct tcggatccaa
     1801 aaaagaagag aaaggtatag aagggtgggc gcgccgaccc agctttcttg tacaaagtgg
     1861 ttgataacag cgcttagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     1921 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     1981 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     2041 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     2101 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttggttccgg
     2161 aaccaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2221 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2281 taactcacat taggctgaat taggcgcgcc tatttctatt aattcccgat ctagtaacat
     2341 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     2401 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     2461 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
     2521 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
     2581 ggggaaattc gagctgggga cgtccccagc tcggtacccc ctcctgggca cggtcgtacg
     2641 cgatccgccg cggttccacc tcaccattgc gggtatgcca gttgagttcg atgagttccc
     2701 gtaccagctc caactcgggc gatgccacgc gcactccgtt cagatctcgg tgacgggcag
     2761 gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca
     2821 gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc
     2881 gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc
     2941 ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg
     3001 gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt
     3061 ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga cgagccaggg
     3121 atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt
     3181 acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga ccgccggcat
     3241 gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca tatcttattg
     3301 ccccccggga tcagatctgg attgagagtg aatatgagac tctaattgga taccgagggg
     3361 aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc
     3421 ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact
     3481 ccagaaaccc gcggctgagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa
     3541 aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca
     3601 tgatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
     3661 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
     3721 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
     3781 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
     3841 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc
     3901 tgagtgcttg cggcagcgtg aagctgatcc gtcgagatta atagaaataa attcagccta
     3961 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     4021 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     4081 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     4141 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     4201 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     4261 acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc
     4321 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     4381 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     4441 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     4501 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     4561 aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     4621 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     4681 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     4741 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     4801 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     4861 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     4921 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     4981 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     5041 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     5101 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     5161 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     5221 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     5281 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     5341 atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt
     5401 cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac
     5461 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     5521 ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5581 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5641 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5701 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5761 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5821 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5881 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5941 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6001 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6061 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6121 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6181 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6241 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6301 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6361 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     6421 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6481 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     6541 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     6601 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     6661 ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc
     6721 cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc
     6781 cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc
     6841 tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta
     6901 ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact
     6961 tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc
     7021 ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga
     7081 cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg
     7141 gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc
     7201 gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg
     7261 aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac
     7321 catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg
     7381 ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct
     7441 gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt
     7501 tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg
     7561 cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg
     7621 caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt
     7681 acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat
     7741 cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca
     7801 ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg
     7861 atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg
     7921 ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg
     7981 tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct
     8041 ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg
     8101 cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc
     8161 gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg
     8221 gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc
     8281 ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca
     8341 tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct
     8401 ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg
     8461 tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct
     8521 ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg
     8581 gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt
     8641 ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg
     8701 attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac
     8761 gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc
     8821 gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct
     8881 caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg
     8941 gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg
     9001 cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt
     9061 caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc
     9121 aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt
     9181 gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa
     9241 gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat
     9301 atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg
     9361 gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga
     9421 caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac
     9481 gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg
     9541 gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg
     9601 gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc
     9661 gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt
     9721 gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc
     9781 tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg
     9841 cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac
     9901 acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta
     9961 cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg
    10021 cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc
    10081 atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg
    10141 gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg
    10201 acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg
    10261 ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa
    10321 aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa
    10381 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa
    10441 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
    10501 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
    10561 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
    10621 atctgatcca agctcaagct gctctagcat tcgccattca ggctgcgcaa ctgttgggaa
    10681 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
    10741 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
    10801 agtgccaagc ttgtggatcc cccatcacaa ctttgtatag aaaagttggc tccgaattcg
    10861 cccttagtct agctcaacag agcttttaac ccaaattggt acaatagaat acaactttag
    10921 atcataattc tcaaaagaaa gagattcctt agctattcta tctgccactc catttccttc
    10981 tcggcttgta tgcacaagca taaaatcctc aaacttgcta agtagatact ttatgtcttg
    11041 gataattgga ttgagacttg acaagcataa ctttcatgta accaaagaca caagttgctg
    11101 agaatccacc tcaaaaatga tcttcctata attgaatcgg gataatgaca gcacagccca
    11161 tctaagagcc tccacttcta cttccagcac gcttcttact tttaccacag ctcttgcacc
    11221 taaccataac accttccctg tatgatcgcg aagcacccac cctaagccac attttaatcc
    11281 ttctgttggc catgccccat caaagttgca cttaacccaa gattgtggtg gagcttccca
    11341 tgtttctcgt ctgtcccgac ggtgttgtgg ttggtgcttt ccttacattc tgagcctctt
    11401 tccttctaat ccactcatct gcatcttctt gtgtccttac taatacctca ttggttccaa
    11461 attccctccc tttaagcacc agctcgtttc tgttcttcca cagcctccca agtatccaag
    11521 ggactaaagc ctccacattc ttcagatcag gatattcttg tttaagatgt tgaactctat
    11581 ggaggtttgt atgaactgat gatctaggac cggataagtt cccttcttca tagcgaactt
    11641 attcaaagaa tgttttgtgt atcattcttg ttacattgtt attaatgaaa aaatattatt
    11701 ggtcattgga ctgaacacga gtgttaaata tggaccaggc cccaaataag atccattgat
    11761 atatgaatta aataacaaga ataaatcgag tcaccaaacc acttgccttt tttaacgaga
    11821 cttgttcacc aacttgatac aaaagtcatt atcctatgca aatcaataat catacaaaaa
    11881 tatccaataa cactaaaaaa ttaaaagaaa tggataattt cacaatatgt tatacgataa
    11941 agaagttact tttccaagaa attcactgat tttataagcc cacttgcatt agataaatgg
    12001 caaaaaaaaa caaaaaggaa aagaaataaa gcacgaagaa ttctagaaaa tacgaaatac
    12061 gcttcaatgc agtgggaccc acggttcaat tattgccaat tttcagctcc accgtatatt
    12121 taaaaaataa aacgataatg ctaaaaaaat ataaatcgta acgatcgtta aatctcaacg
    12181 gctggatctt atgacgaccg ttagaaattg tggttgtcga cgagtcagta ataaacggcg
    12241 tcaaagtggt tgcagccggc acacacgagt cgtgtttatc aactcaaagc acaaatactt
    12301 ttcctcaacc taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa acaacgctca
    12361 atacacgtgt cattttatta ttagctattg cttcaccgcc ttagctttct cgtgacctag
    12421 tcgtcctcgt cttttcttct tcttcttcta taaaacaata cccaaagagc tcttcttctt
    12481 cacaattcag atttcaattt ctcaaaatct taaaaacttt ctctcaattc tctctaccgt
    12541 gatcaaggta aatttctgtg ttccttattc tctcaaaatc ttcgattttg ttttcgttcg
    12601 atcccaattt cgtatatgtt ctttggttta gattctgtta atcttagatc gaagacgatt
    12661 ttctgggttt gatcgttaga tatcatctta attctcgatt agggtttcat agatatcatc
    12721 cgatttgttc aaataatttg agttttgtcg aataattact cttcgatttg tgatttctat
    12781 ctagatctgg tgttagtttc tagtttgtgc gatcgaattt gtcgattaat ctgagttttt
    12841 ctgattaaca gaagggcgaa ttcgac
//
