LOCUS       sequence-246290-       15572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15572)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX012
FEATURES             Location/Qualifiers
     source          1..15572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5718..5742)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5771..5860
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5894..6146
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6575..6827)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7295..8320)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7295..8320)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8330..8674)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8784..8801)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8784..8800)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8793..8815)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8940..8959)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9150..9171
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9418..9442
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11038..12186)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12485..13279)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13471..14238
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14931..15562
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggcagc
     4621 agctatggaa ttttggaata gtacatctgt agatgttcac tcaacaccag aacctattat
     4681 tacttctggt ggtgaattaa tggaagcact tgaacctttt atgaaaagtg cttcttcttc
     4741 tccttcttct aatttccctc cttctatttc tactcctttc ccttcttctg attttcactc
     4801 tcttccttca tttcctcctc ctcttcctac ccctacttct accatttcat atccttgcac
     4861 ttttttttac caaacagcac ctcaacctat gggctctgaa tcttgttcma cttccactac
     4921 tgaaaacacc caaatctttt caactgggtt ttcgggttat ggaatggagc aaccaggctc
     4981 aatcgggttg aatcaattaa ccccagctca gatccaacaa attcaagccc aaatcaactt
     5041 ccaaacccaa caacaacacc agatgatgtt ccatgatggg taccaccacc atgcatctac
     5101 tatgaacttg ttgggcccaa agcccgttca aatgaagcaa tcgggctcac caccaaagcc
     5161 cactaagctc tacagaggcg ttagacaacg tcactgggga aagtgggtcg ctgaaatccg
     5221 attgcccaag aaccggaccc ggctttggct tggcaccttt gacactgctg aagaagctgc
     5281 gttggcctat gacaaggcgg cgtatatgct gcgtggcgac tttgctagac tcaactttcc
     5341 ccatctccgc cacaacggtt cccttatcgg cggcgaattc ggagagtaca atccattgca
     5401 ttcctctgtt gatgctaagt tgaaggagat ttgcgagagc ttggcgcagg ggaagagcat
     5461 tgactctagg aagaagaaga cgtccaaagg gtcttccgcg gccgcgttgg tgaaaatgga
     5521 ggaagaggag agcaaggtgg tggaaggtgg atccgagagt gacgggtcgg gttcagggtc
     5581 aggtggatcc tcgcccgtaa ctgacctgac atttccggag tttactcagg aagagtcaac
     5641 gtgggacatg tgttcggaga atacgttgtt gcagaagtat ccatctcatg agattgattg
     5701 ggccgcttta cccggggacc cagctttctt gtacaaagtg gttgataaca gcgggttaat
     5761 taacatcttt tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga
     5821 ctatgcagga tcctatccat atgacgttcc agattacgct gctcagtgca gcgcttagag
     5881 ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     5941 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     6001 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     6061 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     6121 cgcggtgtca tctatgttac tagatcggga attagcttca tcaacgcaag acatgcgcac
     6181 gaccgtctga caggagagga atttccgacg agcacagaaa ggacttgctc ttggacgtag
     6241 gcctatttct caggcacatg tatcaagtgt tcggacgtgg gttttcgatg gtgtatcagc
     6301 cgccgccaac tgggagatga ggaggctttc ttggggggca gtcagcagtt catttcacaa
     6361 gacagaggaa cttgtaagga gatgcactga tttatcttgg cgcaaaccag caggacgaat
     6421 tagtgggaat agcccgcgaa tatctaagtt atgcctgtcg gcatgagcag aaacttccaa
     6481 ttcgaaacag tttggagagg ttgtttttgg gcataccttt tgttagtcag cctctcgatt
     6541 gctcatcgtc attacacagt accgaagttt gatcgatcta gtaacataga tgacaccgcg
     6601 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     6661 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     6721 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
     6781 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttcgacgcac
     6841 tccttcttta ctccaccatc tcgtccttat tgaaaacgtg ggtagcacca aaacgaatca
     6901 agtcgctgga actgaagtta ccaatcacgc tggatgattt gccagttgga ttaatcttgc
     6961 ctttccccgc atgaataata ttgatgaatg catgcgtgag gggtatttcg attttggcaa
     7021 tagctgcaat tgccgcgaca tcctccaacg agcataattc ttcagaaaaa tagcgatgtt
     7081 ccatgttgtc agggcatgca tgatgcacgt tatgaggtga cggtgctagg cagtattccc
     7141 tcaaagtttc atagtcagta tcatattcat cattgcattc ctgcaagaga gaattgagac
     7201 gcaatccaca cgctgcggca accttccggc gttcgtggtc tatttgctct tggacgttgc
     7261 aaacgtaagt gttggatccc ggtcggcatc tactctattc ctttgccctc ggacgagtgc
     7321 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
     7381 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
     7441 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
     7501 gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
     7561 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     7621 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     7681 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     7741 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     7801 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
     7861 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
     7921 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg
     7981 gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
     8041 ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa
     8101 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
     8161 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
     8221 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
     8281 acttctcgac agacgtcgcg gtgagttcag gctttttcat atcggggtcg tcctctccaa
     8341 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
     8401 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
     8461 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     8521 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     8581 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     8641 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     8701 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttga cggatctcta
     8761 ggacgcgtcc tagaagctaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     8821 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     8881 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgcccgc
     8941 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9001 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9061 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9121 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9181 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggaaccacc
     9241 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     9301 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     9361 accccagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     9421 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     9481 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     9541 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     9601 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     9661 agcgttgctg cctgtgatca aatatcatct ccctcgcaga gatccgaatt atcagccttc
     9721 ttattcattt ctcgcttaac cgtgacaggc tgtcgatctt gagaactatg ccgacataat
     9781 aggaaatcgc tggataaagc cgctgaggaa gctgagtggc gctatttctt tagaagtgaa
     9841 cgttgacgat atcaactccc ctatccattg ctcaccgaat ggtacaggtc ggggacccga
     9901 agttccgact gtcggcctga tgcatccccg gctgatcgac cccagatctg gggctgagaa
     9961 agcccagtaa ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt
    10021 aggttaaacc cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag
    10081 gcatcgggat tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg
    10141 ccagttgcca ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca
    10201 cggttccgaa cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta
    10261 gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca
    10321 ggaccgccat caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca
    10381 gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa
    10441 acaacaagct ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc
    10501 agattcccgt tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc
    10561 gcagcagcat accggcgacc cctcggcctc gctgttcggg ctccacgaaa acgccggaca
    10621 gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac agcccaatga
    10681 tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg caaccgttca gcgaacgcct
    10741 ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa catctctgga gctttcttca
    10801 gggccgacaa tcggatctcg cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct
    10861 gagccttaat cacaattgtc aattttaatc ctctgtttat cggcagttcg tagagcgcgc
    10921 cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg
    10981 aaatgccagt aaagcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta
    11041 gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg
    11101 caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga
    11161 tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa
    11221 atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt
    11281 cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg
    11341 ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag
    11401 gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc
    11461 ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta
    11521 gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg
    11581 ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc
    11641 cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg
    11701 cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca
    11761 cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag
    11821 caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg
    11881 cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc
    11941 ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg
    12001 agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac
    12061 ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc
    12121 ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg
    12181 attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc
    12241 ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc
    12301 ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac
    12361 gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca
    12421 cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta
    12481 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
    12541 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
    12601 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
    12661 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
    12721 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
    12781 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
    12841 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
    12901 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
    12961 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
    13021 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
    13081 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
    13141 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
    13201 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    13261 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
    13321 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    13381 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    13441 gacggagccg attttgaaac cacaattatg ggtgatgctg ccaacttact gatttagtgt
    13501 atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc
    13561 agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg
    13621 ctctcactgc cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac
    13681 gcaccagaaa atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat
    13741 tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta
    13801 acctcgcgca tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg
    13861 ggctatgtcg gggctaaatc gcgccagcgc tggctgtttt acgcgtatga cagtctccgg
    13921 aagacggttg ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg
    13981 agcctgctgt caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa
    14041 tcccgcctga agggaaagct gcacgtaatc agcaagcgat atacgcagcg aattgagcgg
    14101 cataacctga atctgaggca gcacctggca cggctgggac ggaagtcgct gtcgttctca
    14161 aaatcggtgg agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcaa
    14221 taagttggag tcattaccca attatgatag aatttacaag ctataaggtt attgtcctgg
    14281 gtttcaagca ttagtccatg caagttttta tgctttgccc attctataga tatattgata
    14341 agcgcgctgc ctatgccttg ccccctgaaa tccttacata cggcgatatc ttctatataa
    14401 aagatatatt atcttatcag tattgtcaat atattcaagg caatctgcct cctcatcctc
    14461 ttcatcctct tcgtcttggt agctttttaa atatggcgct tcatagagta attctgtaaa
    14521 ggtccaattc tcgttttcat acctcggtat aatcttacct atcacctcaa atggttcgct
    14581 gggtttatcg cacccccgaa cacgagcacg gcacccgcga ccactatgcc aagaatgccc
    14641 aaggtaaaaa ttgccggccc cgccatgaag tccgtgaatg ccccgacggc cgaagtgaag
    14701 ggcaggccgc cacccaggcc gccgccctca ctgcccggca cctggtcgct gaatgtcgat
    14761 gccagcacct gcggcacgtc aatgcttccg ggcgtcgcgc tcgggctgat cgcccatccc
    14821 gttactgccc cgatcccggc aatggcaagg actgccagcg ctgccatttt tggggtgagg
    14881 ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg
    14941 gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca
    15001 gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta
    15061 gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag
    15121 cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa
    15181 ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact
    15241 tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc
    15301 gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt
    15361 caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt
    15421 gagggccaag ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg
    15481 cttcgacggc gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag
    15541 ggcaaccagc ccggtgagcg tcggaaaggc gc
//
