LOCUS       sequence-246293-        3368 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human N-alpha-2M (from S24 to E908) in insect cells. With 
            C-t H6x tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Marino-Puertas L, Del Amo-Maestro L, Taules M, Gomis-Ruth FX, Goulas
            T
  TITLE     Recombinant production of human alpha2-macroglobulin variants and 
            interaction studies with recombinant G-related alpha2-macroglobulin 
            binding protein and latent transforming growth factor-beta2.
  JOURNAL   Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.1038/s41598-019-45712-z.
  PUBMED    31235767
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-06-24"; volume: "9(1):9186. doi"; pages: " 
            10.1038/s41598-019-45712-z"
COMMENT     Sequence Label: pIE-N-h-alpha-2M-H6
FEATURES             Location/Qualifiers
     source          1..3368
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..592
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             3316..3333
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgatgtcttt gtgatgcgcg cgacattttt gtaggttatt gataaaatga acggatacgt
       61 tgcccgacat tatcattaaa tccttggcgt agaatttgtc gggtccattg tccgtgtgcg
      121 ctagcatgcc cgtaacggac ctcgtacttt tggcttcaaa ggttttgcgc acagacaaaa
      181 tgtgccacac ttgcagctct gcatgtgtgc gcgttaccac aaatcccaac ggcgcagtgt
      241 acttgttgta tgcaaataaa tctcgataaa ggcgcggcgc gcgaatgcag ctgatcacgt
      301 acgctcctcg tgttccgttc aaggacggtg ttatcgacct cagattaatg tttatcggcc
      361 gactgttttc gtatccgctc accaaacgcg tttttgcatt aacattgtat gtcggcggat
      421 gttctatatc taatttgaat aaataaacga taaccgcgtt ggttttagag ggcataataa
      481 aagaaatatt gttatcgtgt tcgccattag ggcagtataa attgacgttc atgttggata
      541 ttgtttcagt tgcaagttga cactggcggc gacaagatcg tgaacaacca agtgactatg
      601 tacaagctca cagtcttcct gatgttcatc gctttcgtca tcatcgctga ggcctcagtc
      661 tctggaaaac cgcagtatat ggttctggtc ccctccctgc tccacactga gaccactgag
      721 aagggctgtg tccttctgag ctacctgaat gagacagtga ctgtaagtgc ttccttggag
      781 tctgtcaggg gaaacaggag cctcttcact gacctggagg cggagaatga cgtactccac
      841 tgtgtcgcct tcgctgtccc aaagtcttca tccaatgagg aggtaatgtt cctcactgtc
      901 caagtgaaag gaccaaccca agaatttaag aagcggacca cagtgatggt taagaacgag
      961 gacagtctgg tctttgtcca gacagacaaa tcaatctaca aaccagggca gacagtgaaa
     1021 tttcgtgttg tctccatgga tgaaaacttt caccccctga atgagttgat tccactagta
     1081 tacattcagg atcccaaagg aaatcgcatc gcacaatggc agagtttcca gttagagggt
     1141 ggcctcaagc aattttcttt tcccctctca tcagagccct tccagggctc ctacaaggtg
     1201 gtggtacaga agaaatcagg tggaaggaca gagcaccctt tcaccgtgga ggaatttgtt
     1261 cttcccaagt ttgaagtaca agtaacagtg ccaaagataa tcaccatctt ggaagaagag
     1321 atgaatgtat cagtgtgtgg cctatacaca tatgggaagc ctgtccctgg acatgtgact
     1381 gtgagcattt gcagaaagta tagtgacgct tccgactgcc acggtgaaga ttcacaggct
     1441 ttctgtgaga aattcagtgg acagctaaac agccatggct gcttctatca gcaagtaaaa
     1501 accaaggtct tccagctgaa gaggaaggag tatgaaatga aacttcacac tgaggcccag
     1561 atccaagaag aaggaacagt ggtggaattg actggaaggc agtccagtga aatcacaaga
     1621 accataacca aactctcatt tgtgaaagtg gactcacact ttcgacaggg aattcccttc
     1681 tttgggcagg tgcgcctagt agatgggaaa ggcgtcccta taccaaataa agtcatattc
     1741 atcagaggaa atgaagcaaa ctattactcc aatgctacca cggatgagca tggccttgta
     1801 cagttctcta tcaacaccac caatgttatg ggtacctctc ttactgttag ggtcaattac
     1861 aaggatcgta gtccctgtta cggctaccag tgggtgtcag aagaacacga agaggcacat
     1921 cacactgctt atcttgtgtt ctccccaagc aagagctttg tccaccttga gcccatgtct
     1981 catgaactac cctgtggcca tactcagaca gtccaggcac attatattct gaatggaggc
     2041 accctgctgg ggctgaagaa gctctccttc tattatctga taatggcaaa gggaggcatt
     2101 gtccgaactg ggactcatgg actgcttgtg aagcaggaag acatgaaggg ccatttttcc
     2161 atctcaatcc ctgtgaagtc agacattgct cctgtcgctc ggttgctcat ctatgctgtt
     2221 ttacctaccg gggacgtgat tggggattct gcaaaatatg atgttgaaaa ttgtctggcc
     2281 aacaaggtgg atttgagctt cagcccatca caaagtctcc cagcctcaca cgcccacctg
     2341 cgagtcacag cggctcctca gtccgtctgc gccctccgtg ctgtggacca aagcgtgctg
     2401 ctcatgaagc ctgatgctga gctctcggcg tcctcggttt acaacctgct accagaaaag
     2461 gacctcactg gcttccctgg gcctttgaat gaccaggacg atgaagactg catcaatcgt
     2521 cataatgtct atattaatgg aatcacatat actccagtat caagtacaaa tgaaaaggat
     2581 atgtacagct tcctagagga catgggctta aaggcattca ccaactcaaa gattcgtaaa
     2641 cccaaaatgt gtccacagct tcaacagtat gaaatgcatg gacctgaagg tctacgtgta
     2701 ggtttttatg agtcagatgt aatgggaaga ggccatgcac gcctggtgca tgttgaagag
     2761 cctcacacgg agaccgtacg aaagtacttc cctgagacat ggatctggga tttggtggtg
     2821 gtaaactcag caggtgtggc tgaggtagga gtaacagtcc ctgacaccat caccgagtgg
     2881 aaggcagggg ccttctgcct gtctgaagat gctggacttg gtatctcttc cactgcctct
     2941 ctccgagcct tccagccctt ctttgtggag ctcacaatgc cttactctgt gattcgtgga
     3001 gaggccttca cactcaaggc cacggtccta aactaccttc ccaaatgcat ccgggtcagt
     3061 gtgcagctgg aagcctctcc cgccttccta gctgtcccag tggagaagga acaagcgcct
     3121 cactgcatct gtgcaaacgg gcggcaaact gtgtcctggg cagtaacccc aaagtcatta
     3181 ggaaatgtga atttcactgt gagcgcagag gcactagagt ctcaagagct gtgtgggact
     3241 gaggtgcctt cagttcctga acacggaagg aaagacacag tcatcaagcc tctgttggtt
     3301 gaacctgaaa ccggtcatca tcaccatcac cattgagttt ggcaaggccg gatccgttct
     3361 accctgcg
//
