LOCUS       sequence-246300-        2768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human C-alpha-2M (from R787 to N1473) in insect cells. 
            With C-t H6x tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Marino-Puertas L, Del Amo-Maestro L, Taules M, Gomis-Ruth FX, Goulas
            T
  TITLE     Recombinant production of human alpha2-macroglobulin variants and 
            interaction studies with recombinant G-related alpha2-macroglobulin 
            binding protein and latent transforming growth factor-beta2.
  JOURNAL   Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.1038/s41598-019-45712-z.
  PUBMED    31235767
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-06-24"; volume: "9(1):9186. doi"; pages: " 
            10.1038/s41598-019-45712-z"
COMMENT     Sequence Label: pIE-C-h-alpha-2M-H6
FEATURES             Location/Qualifiers
     source          1..2768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..592
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             2716..2733
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgatgtcttt gtgatgcgcg cgacattttt gtaggttatt gataaaatga acggatacgt
       61 tgcccgacat tatcattaaa tccttggcgt agaatttgtc gggtccattg tccgtgtgcg
      121 ctagcatgcc cgtaacggac ctcgtacttt tggcttcaaa ggttttgcgc acagacaaaa
      181 tgtgccacac ttgcagctct gcatgtgtgc gcgttaccac aaatcccaac ggcgcagtgt
      241 acttgttgta tgcaaataaa tctcgataaa ggcgcggcgc gcgaatgcag ctgatcacgt
      301 acgctcctcg tgttccgttc aaggacggtg ttatcgacct cagattaatg tttatcggcc
      361 gactgttttc gtatccgctc accaaacgcg tttttgcatt aacattgtat gtcggcggat
      421 gttctatatc taatttgaat aaataaacga taaccgcgtt ggttttagag ggcataataa
      481 aagaaatatt gttatcgtgt tcgccattag ggcagtataa attgacgttc atgttggata
      541 ttgtttcagt tgcaagttga cactggcggc gacaagatcg tgaacaacca agtgactatg
      601 tacaagctca cagtcttcct gatgttcatc gctttcgtca tcatcgctga ggcctcacag
      661 cccttctttg tggagctcac aatgccttac tctgtgattc gtggagaggc cttcacactc
      721 aaggccacgg tcctaaacta ccttcccaaa tgcatccggg tcagtgtgca gctggaagcc
      781 tctcccgcct tcctagctgt cccagtggag aaggaacaag cgcctcactg catctgtgca
      841 aacgggcggc aaactgtgtc ctgggcagta accccaaagt cattaggaaa tgtgaatttc
      901 actgtgagcg cagaggcact agagtctcaa gagctgtgtg ggactgaggt gccttcagtt
      961 cctgaacacg gaaggaaaga cacagtcatc aagcctctgt tggttgaacc tgaaggacta
     1021 gagaaggaaa caacattcaa ctccctactt tgtccatcag gtggtgaggt ttctgaagaa
     1081 ttatccctga aactgccacc aaatgtggta gaagaatctg cccgagcttc tgtctcagtt
     1141 ttgggagaca tattaggctc tgccatgcaa aacacacaaa atcttctcca gatgccctat
     1201 ggctgtggag agcagaatat ggtcctcttt gctcctaaca tctatgtact ggattatcta
     1261 aatgaaacac agcagcttac tccagagatc aagtccaagg ccattggcta tctcaacact
     1321 ggttaccaga gacagttgaa ctacaaacac tatgatggct cctacagcac ctttggggag
     1381 cgatatggca ggaaccaggg caacacctgg ctcacagcct ttgttctgaa gacttttgcc
     1441 caagctcgag cctacatctt catcgatgaa gcacacatta cccaagccct catatggctc
     1501 tcccagaggc agaaggacaa tggctgtttc aggagctctg ggtcactgct caacaatgcc
     1561 ataaagggag gagtagaaga tgaagtgacc ctctccgcct atatcaccat cgcccttctg
     1621 gagattcctc tcacagtcac tcaccctgtt gtccgcaatg ccctgttttg cctggagtca
     1681 gcctggaaga cagcacaaga aggggaccat ggcagccatg tatataccaa agcactgctg
     1741 gcctatgctt ttgccctggc aggtaaccag gacaagagga aggaagtact caagtcactt
     1801 aatgaggaag ctgtgaagaa agacaactct gtccattggg agcgccctca gaaacccaag
     1861 gcaccagtgg ggcattttta cgaaccccag gctccctctg ctgaggtgga gatgacatcc
     1921 tatgtgctcc tcgcttatct cacggcccag ccagccccaa cctcggagga cctgacctct
     1981 gcaaccaaca tcgtgaagtg gatcacgaag cagcagaatg cccagggcgg tttctcctcc
     2041 acccaggaca cagtggtggc tctccatgct ctgtccaaat atggagcagc cacatttacc
     2101 aggactggga aggctgcaca ggtgactatc cagtcttcag ggacattttc cagcaaattc
     2161 caagtggaca acaacaaccg cctgttactg cagcaggtct cattgccaga gctgcctggg
     2221 gaatacagca tgaaagtgac aggagaagga tgtgtctacc tccagacatc cttgaaatac
     2281 aatattctcc cagaaaagga agagttcccc tttgctttag gagtgcagac tctgcctcaa
     2341 acttgtgatg aacccaaagc ccacaccagc ttccaaatct ccctaagtgt cagttacaca
     2401 gggagccgct ctgcctccaa catggcgatc gttgatgtga agatggtctc tggcttcatt
     2461 cccctgaagc caacagtgaa aatgcttgaa agatctaacc atgtgagccg gacagaagtc
     2521 agcagcaacc atgtcttgat ttaccttgat aaggtgtcaa atcagacact gagcttgttc
     2581 ttcacggttc tgcaagatgt cccagtaaga gatctgaaac cagccatagt gaaagtctat
     2641 gattactacg agacggatga gtttgcaatt gctgagtaca atgctccttg cagcaaagat
     2701 cttggaaata ccggtcatca tcaccatcac cattgagttt ggcaaggccg gatccgttct
     2761 accctgcg
//
