LOCUS       sequence-246323-        7377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SomArchon-P2A-CoChR-Kv2.1 under Syn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Piatkevich KD, Bensussen S, Tseng HA, Shroff SN, Lopez-Huerta VG, 
            Park D, Jung EE, Shemesh OA, Straub C, Gritton HJ, Romano MF, Costa 
            E, Sabatini BL, Fu Z, Boyden ES, Han X
  TITLE     Population imaging of neural activity in awake behaving mice.
  JOURNAL   Nature. 2019 Oct;574(7778):413-417. doi: 10.1038/s41586-019-1641-1. 
            Epub 2019 Oct 9.
  PUBMED    31597963
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1641-1"; journalName: "Nature"; date: "2019-10";
            volume: "574"; issue: "7778"; pages: "413-417"
COMMENT     Sequence Label: pAAV-Syn-SomArchon-P2A-CoChR-Kv2.1
FEATURES             Location/Qualifiers
     source          1..7377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   41..170
                     /label=AAV2 ITR
     promoter        273..720
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      733..742
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1606..1608,1609..2319)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1606..1608,1609..2319)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1648..1669)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1909..1928)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2256..2277
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2575..2631
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2632..3495
                     /codon_start=1
                     /product="high-photocurrent channelrhodopsin from 
                     Chloromonas oogama (Shemesh et al., 2017)"
                     /label=CoChR
                     /translation="MLGNGSAIVPIDQCFCLAWTDSLGSDTEQLVANILQWFAFGFSIL
                     ILMFYAYQTWRATCGWEEVYVCCVELTKVIIEFFHEFDDPSMLYLANGHRVQWLRYAEW
                     LLTCPVILIHLSNLTGLKDDYSKRTMRLLVSDVGTIVWGATSAMSTGYVKVIFFVLGCI
                     YGANTFFHAAKVYIESYHVVPKGRPRTVVRIMAWLFFLSWGMFPVLFVVGPEGFDAISV
                     YGSTIGHTIIDLMSKNCWGLLGHYLRVLIHQHIIIYGDIRKKTKINVAGEEMEVETMVD
                     QEDEETV"
     misc_feature    3769..4357
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3822..3842)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4240..4251)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4398..4605
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4775..4904
                     /label=AAV2 ITR
     rep_origin      complement(5141..5729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5221..5240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5900..6691,6692..6760))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6523..6542
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6761..6865)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6891..7346)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7028..7049)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7240..7259
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctgcgcgctc gctcgctcac
       61 tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag
      121 cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct tgtagttaat
      181 gattaacccg ccatgctact tatctacgta gccatgctct aggaagatcg taggttaatt
      241 aagtgtctag actgcagagg gccctgcgta tgagtgcaag tgggttttag gaccaggatg
      301 aggcggggtg ggggtgccta cctgacgacc gaccccgacc cactggacaa gcacccaacc
      361 cccattcccc aaattgcgca tcccctatca gagaggggga ggggaaacag gatgcggcga
      421 ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc cttcgccccc gcctggcggc
      481 gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt cactcgccgg tcccccgcaa
      541 actccccttc ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc cggaccgcac
      601 cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg cgccggcgac
      661 tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct gagagcgcag
      721 tcgagaggat ccgccaccat ggtttctatc gcactgcagg ctggttatga cctcctcgga
      781 gatggtagac ctgaaagtct gtggctcgga attggtacac tgctgatgct gatcggaacc
      841 ttctacttcc tggttagagc gtggggagag actgacaagg atgcacggga gtactacgca
      901 gtgacaatcc tggtcagcgg tattgccagt gcagcttatc tgtcaatgtt ctttggtatt
      961 ggcctcacag aggtgccagt aggtggagag atgctgaaca tctactacgc aagatacgca
     1021 cagtggctgt tcactacacc actgttgctg ctgcacctgg cactcctggc caaagttgat
     1081 agagtgacca tcggcactct cgttggcgtc gatgctctga tgattgtcac aggcctgata
     1141 ggaacactgt cacatactgc cattgccaga tactcttggt ggctgttctc aactatctgt
     1201 atgatcgtcg ttctgtatgt cctggccact tctctgcggt cagccgctaa ggagagaggc
     1261 ccagaggttg cctcaacctt caacatcctc accgcactgg ttctggtcct gtggactgct
     1321 taccctatca tctggattat cggcaccgag ggtgccggag ttgtgggcct gggaatcgaa
     1381 actttgctgt tcatggtgct ggacgtcacc gccaaggtgg gcttcggttt tatcctgctc
     1441 aggtcaagag ccattctgca agatacagaa gcacctgaac cttcagctgg agctgatacc
     1501 ggtgccgccg accgcccggt agtagcagtg agcaaggcgg ccgccaagag caggatcacc
     1561 agcgagggcg agtacatccc cctggaccag atcgacatca acgtggtgag caagggcgag
     1621 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1681 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1741 ttcatttgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1801 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1861 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1921 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1981 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2041 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     2101 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     2161 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     2221 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2281 gccgccggga tcactctcgg catggacgag ctgtacaagg gtgggagcgg aggtacccag
     2341 agccagccta tcctgaacac caaggagatg gcccctcaga gcaagcctcc tgaggagctg
     2401 gagatgagca gcatgcctag ccctgtggcc cctctgcctg ccaggaccga gggcgtgatc
     2461 gacatgagga gcatgagcag catcgacagc ttcatcagct gcgccaccga cttccctgag
     2521 gccaccaggt tctacaagtt ctgctacgag aacgaggtgg gtgtgggctc cggagccacg
     2581 aacttctctc tgttaaagca agcaggagac gtggaagaaa accccggtcc catgctggga
     2641 aacggcagcg ccattgtgcc tatcgaccag tgcttttgcc tggcttggac cgacagcctg
     2701 ggaagcgata cagagcagct ggtggccaac atcctccagt ggttcgcctt cggcttcagc
     2761 atcctgatcc tgatgttcta cgcctaccag acttggagag ccacttgcgg ttgggaggag
     2821 gtctacgtct gttgcgtcga gctgaccaag gtcatcatcg agttcttcca cgagttcgac
     2881 gaccccagca tgctgtacct ggctaacgga caccgagtcc agtggctgag atacgcagag
     2941 tggctgctga cttgtcccgt catcctgatc cacctgagca acctgaccgg cctgaaggac
     3001 gactacagca agcggaccat gaggctgctg gtgtcagacg tgggaaccat cgtgtgggga
     3061 gctacaagcg ccatgagcac aggctacgtc aaggtcatct tcttcgtgct gggttgcatc
     3121 tacggcgcca acaccttctt ccacgccgcc aaggtgtata tcgagagcta ccacgtggtg
     3181 ccaaagggca gacctagaac cgtcgtgcgg atcatggctt ggctgttctt cctgtcttgg
     3241 ggcatgttcc ccgtgctgtt cgtcgtggga ccagaaggat tcgacgccat cagcgtgtac
     3301 ggctctacca ttggccacac catcatcgac ctcatgagca agaattgttg gggcctgctg
     3361 ggacactatc tgagagtgct gatccaccag cacatcatca tctacggcga catccggaag
     3421 aagaccaaga tcaacgtggc cggcgaggag atggaagtgg agaccatggt ggaccaggag
     3481 gacgaggaga cagtgcttaa gggtgggagc ggaggtaccc agagccagcc tatcctgaac
     3541 accaaggaga tggcccctca gagcaagcct cctgaggagc tggagatgag cagcatgcct
     3601 agccctgtgg cccctctgcc tgccaggacc gagggcgtga tcgacatgag gagcatgagc
     3661 agcatcgaca gcttcatcag ctgcgccacc gacttccctg aggccaccag gttctacaag
     3721 ttctgctacg agaacgaggt gtaagaattc cgtacaaagc ttatcgataa tcaacctctg
     3781 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3841 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3901 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3961 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4021 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4081 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4141 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     4201 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4261 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4321 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acccgggcgg
     4381 ccgctcataa agctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     4441 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4501 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4561 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tccggactgt
     4621 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca
     4681 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatct tcctagagca tggctacgta
     4741 gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc
     4801 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     4861 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcat taatgaatcg
     4921 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4981 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5041 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5581 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     6901 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     6961 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     7021 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     7081 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     7141 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     7201 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     7261 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     7321 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttg
//
