LOCUS       sequence-24633-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     Lowe Lab Plasmids
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe H-Ras V12 G37
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(22..54,55..57,58..591)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIGDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     primer_bind     complement(610..630)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gnngatnnna ttcnctcgac catgacggaa tataagctgg tggtggtggg cgccgtcggt
       61 gtgggcaaga gtgcgctgac catccagctg atccagaacc attttgtgga cgaatacgac
      121 cccactatag gggattccta ccggaagcag gtggtcattg atggggagac gtgcctgttg
      181 gacatcctgg ataccgccgg ccaggaggag tacagcgcca tgcgggacca gtacatgcgc
      241 accggggagg gcttcctgtg tgtgtttgcc atcaacaaca ccaagtcttt tgaggacatc
      301 caccagtaca gggagcagat caaacgggtg aaggactcgg atgacgtgcc catggtgctg
      361 gtggggaaca agtgtgacct ggctgcacgc actgtggaat ctcggcaggc tcaggacctc
      421 gcccgaagct acggcatccc ctacatcgag acctcggcca agacccggca gggagtggag
      481 gatgccttct acacgttggt gcgtgagatc cggcagcaca agctgcggaa gctgaaccct
      541 cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg tgctctcctg aggatctagt
      601 cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc cccnnnnncc cgcaggagaa
      661 nnnnnnnnna anagcnngcn nctcaattag tcagcaacca ggngtgngna aagtccccag
      721 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttantnnnca accatagtcc
      781 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tnntccgccc
      841 ca
//
