LOCUS       sequence-246335-       15857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15857)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX025
FEATURES             Location/Qualifiers
     source          1..15857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6003..6027)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6056..6145
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6179..6431
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6860..7112)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7580..8605)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7580..8605)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8615..8959)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9069..9086)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9069..9085)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9078..9100)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9225..9244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9435..9456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9703..9727
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11323..12471)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12770..13564)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13756..14523
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15216..15847
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg ttggatctga atgtatctgc
     4621 agtttctgtg aactcaaatt gcgatgagac tgaccccaac aacaacacca cccttttctt
     4681 taaagatgaa tattctgctg gaacatccaa caccactgct tcctcctccg tcgtcaacgc
     4741 ccccgtcgcc ggtgacgaag attccaacag ctcctccgtg catcacggtt ctaaatgggc
     4801 ccaccattct ctttcaactc tcaatttctc catactgaag agcgatcggg ccattgatac
     4861 tgaagatgat atgagcactg atgacgtgct gacgaggcag ctttttccgg tgaaaaatga
     4921 atcgcagggg caggggacga ggattcaggg tcaatctcag tgctggctga acttgtctgt
     4981 gccggaatcc ggcggcggag cagaaatggg aatttacaaa ccacctacgc cggcgcaaca
     5041 gccggtgaag aaaagccgac gtgggcccag gtcccgtagc tcacagtatc gtggtgtcac
     5101 attttaccgg cgaactggca gatgggaatc gcatatctgg gattgtggga aacaagtgta
     5161 tttgggagga tttgatactg ctcatgctgc agctagggca tatgatcgag ctgcaattaa
     5221 gttccgggga gttgatgctg atatcaactt caatataact gattatgaag aagatatgga
     5281 acagatgaaa aatttgtcaa aagaagaatt tgttcaagtc cttcgtcgtc aaagcactgg
     5341 attctctagg ggaaattcaa aatacagagg ggtaaccttg cacaaatgtg gtcgctggga
     5401 ggctcgtgtg ggtcagttcc taggaaaaaa ggcttatgac aaggcagcca tcaaatgtaa
     5461 tggaagggaa gcggttacca gctttgagcc aaatacatat gaacgggata tgaaggagga
     5521 taataaagat ggtagtggtc agaatcttga tctgaacctc tggatttctc cacctttggc
     5581 tggtccaaag cgtgatgaaa ttggtagaaa cgtacccttc aattttggag ctcatgaaat
     5641 ggttattgga agaagattag aggtagagaa ctcctctact gctccacaag gtgcaccaac
     5701 ggcttctaag ctgtccgtat tgactggcgt gtatcctggt ttttctccgc acaatatgga
     5761 aggagcaatg gcgaaggttg aagcaacttc ctctccagga ttcccaaatt gggcttggaa
     5821 aatacatagc cagggtttaa tcactccagt gcccgtgttt tcttcttctg cagcatcatc
     5881 aggattctct accactacca ctctgtatcc gagtaccctc ctactgccaa gcaaccaact
     5941 acgactcccc acaaactcga ctttccaaca ccctcccgtc acccttaaca tcagtcccgg
     6001 ggacccagct ttcttgtaca aagtggttga taacagcggg ttaattaaca tcttttaccc
     6061 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta
     6121 tccatatgac gttccagatt acgctgctca gtgcagcgct tagagctcga atttccccga
     6181 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     6241 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     6301 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     6361 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     6421 gttactagat cgggaattag cttcatcaac gcaagacatg cgcacgaccg tctgacagga
     6481 gaggaatttc cgacgagcac agaaaggact tgctcttgga cgtaggccta tttctcaggc
     6541 acatgtatca agtgttcgga cgtgggtttt cgatggtgta tcagccgccg ccaactggga
     6601 gatgaggagg ctttcttggg gggcagtcag cagttcattt cacaagacag aggaacttgt
     6661 aaggagatgc actgatttat cttggcgcaa accagcagga cgaattagtg ggaatagccc
     6721 gcgaatatct aagttatgcc tgtcggcatg agcagaaact tccaattcga aacagtttgg
     6781 agaggttgtt tttgggcata ccttttgtta gtcagcctct cgattgctca tcgtcattac
     6841 acagtaccga agtttgatcg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     6901 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     6961 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     7021 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     7081 aagaaacttt attgccaaat gtttgaacga tctgcttcga cgcactcctt ctttactcca
     7141 ccatctcgtc cttattgaaa acgtgggtag caccaaaacg aatcaagtcg ctggaactga
     7201 agttaccaat cacgctggat gatttgccag ttggattaat cttgcctttc cccgcatgaa
     7261 taatattgat gaatgcatgc gtgaggggta tttcgatttt ggcaatagct gcaattgccg
     7321 cgacatcctc caacgagcat aattcttcag aaaaatagcg atgttccatg ttgtcagggc
     7381 atgcatgatg cacgttatga ggtgacggtg ctaggcagta ttccctcaaa gtttcatagt
     7441 cagtatcata ttcatcattg cattcctgca agagagaatt gagacgcaat ccacacgctg
     7501 cggcaacctt ccggcgttcg tggtctattt gctcttggac gttgcaaacg taagtgttgg
     7561 atcccggtcg gcatctactc tattcctttg ccctcggacg agtgctgggg cgtcggtttc
     7621 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     7681 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     7741 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     7801 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga
     7861 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
     7921 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
     7981 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
     8041 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
     8101 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
     8161 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
     8221 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg
     8281 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
     8341 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg
     8401 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
     8461 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
     8521 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
     8581 tcgcggtgag ttcaggcttt ttcatatcgg ggtcgtcctc tccaaatgaa atgaacttcc
     8641 ttatatagag gaagggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt
     8701 ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     8761 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     8821 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     8881 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     8941 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     9001 ggagtagacg agagtgtcgt gctccaccat gttgacggat ctctaggacg cgtcctagaa
     9061 gctaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     9121 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     9181 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg cccgctcctt tcgctttctt
     9241 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     9301 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     9361 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     9421 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     9481 ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa acaggatttt
     9541 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     9601 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccacccc agtacattaa
     9661 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
     9721 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
     9781 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
     9841 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
     9901 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
     9961 gatcaaatat catctccctc gcagagatcc gaattatcag ccttcttatt catttctcgc
    10021 ttaaccgtga caggctgtcg atcttgagaa ctatgccgac ataataggaa atcgctggat
    10081 aaagccgctg aggaagctga gtggcgctat ttctttagaa gtgaacgttg acgatatcaa
    10141 ctcccctatc cattgctcac cgaatggtac aggtcgggga cccgaagttc cgactgtcgg
    10201 cctgatgcat ccccggctga tcgaccccag atctggggct gagaaagccc agtaaggaaa
    10261 caactgtagg ttcgagtcgc gagatccccc ggaaccaaag gaagtaggtt aaacccgctc
    10321 cgatcaggcc gagccacgcc aggccgagaa cattggttcc tgtaggcatc gggattggcg
    10381 gatcaaacac taaagctact ggaacgagca gaagtcctcc ggccgccagt tgccaggcgg
    10441 taaaggtgag cagaggcacg ggaggttgcc acttgcgggt cagcacggtt ccgaacgcca
    10501 tggaaaccgc ccccgccagg cccgctgcga cgccgacagg atctagcgct gcgtttggtg
    10561 tcaacaccaa cagcgccacg cccgcagttc cgcaaatagc ccccaggacc gccatcaatc
    10621 gtatcgggct acctagcaga gcggcagaga tgaacacgac catcagcggc tgcacagcgc
    10681 ctaccgtcgc cgcgaccccg cccggcaggc ggtagaccga aataaacaac aagctccaga
    10741 atagcgaaat attaagtgcg ccgaggatga agatgcgcat ccaccagatt cccgttggaa
    10801 tctgtcggac gatcatcacg agcaataaac ccgccggcaa cgcccgcagc agcataccgg
    10861 cgacccctcg gcctcgctgt tcgggctcca cgaaaacgcc ggacagatgc gccttgtgag
    10921 cgtccttggg gccgtcctcc tgtttgaaga ccgacagccc aatgatctcg ccgtcgatgt
    10981 aggcgccgaa tgccacggca tctcgcaacc gttcagcgaa cgcctccatg ggctttttct
    11041 cctcgtgctc gtaaacggac ccgaacatct ctggagcttt cttcagggcc gacaatcgga
    11101 tctcgcggaa atcctgcacg tcggccgctc caagccgtcg aatctgagcc ttaatcacaa
    11161 ttgtcaattt taatcctctg tttatcggca gttcgtagag cgcgccgtgc gtcccgagcg
    11221 atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt tcctgaaatg ccagtaaagc
    11281 gctggctgct gaacccccag ccggaactga ccccacaagg ccctagcgtt tgcaatgcac
    11341 caggtcatca ttgacccagg cgtgttccac caggccgctg cctcgcaact cttcgcaggc
    11401 ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc acatgaggcg
    11461 gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg
    11521 tcgggccgtc ggcgacagct tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc
    11581 agcaaacagc acgacgattt cctcgtcgat caggacctgg caacgggacg ttttcttgcc
    11641 acggtccagg acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc
    11701 ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta
    11761 ataccggcca ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat
    11821 cggctcgccg ataggggtgc gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc
    11881 atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa
    11941 aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc
    12001 agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa
    12061 caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt
    12121 ggcctcgctg acctgttttg ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat
    12181 agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct gttcgagacg
    12241 acgcgaacgc tccacggcgg ccgatggcgc gggcagggca gggggagcca gttgcacgct
    12301 gtcgcgctcg atcttggccg tagcttgctg gaccatcgag ccgacggact ggaaggtttc
    12361 gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc
    12421 cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc
    12481 ttgcgggatt gccccgactc acgccggggc aatgtgccct tattcctgat ttgacccgcc
    12541 tggtgccttg gtgtccagat aatccacctt atcggcaatg aagtcggtcc cgtagaccgt
    12601 ctggccgtcc ttctcgtact tggtattccg aatcttgccc tgcacgaata ccagcgaccc
    12661 cttgcccaaa tacttgccgt gggcctcggc ctgagagcca aaacacttga tgcggaagaa
    12721 gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac atctaggtac taaaacaatt
    12781 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
    12841 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
    12901 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
    12961 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
    13021 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
    13081 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
    13141 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
    13201 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
    13261 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
    13321 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
    13381 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
    13441 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
    13501 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
    13561 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
    13621 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
    13681 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
    13741 gaaaccacaa ttatgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg
    13801 aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt
    13861 ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat ctctgctctc actgccgtaa
    13921 aacatggcaa ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat
    13981 tgatatggcc atgaatggcg ttggatgccg ggcaacagcc cgcattatgg gcgttggcct
    14041 caacacgatt ttacgtcact taaaaaactc aggccgcagt cggtaacctc gcgcatacag
    14101 ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac agtggggcta tgtcggggct
    14161 aaatcgcgcc agcgctggct gttttacgcg tatgacagtc tccggaagac ggttgttgcg
    14221 cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct gctgtcaccc
    14281 tttgacgtgg tgatatggat gacggatggc tggccgctgt atgaatcccg cctgaaggga
    14341 aagctgcacg taatcagcaa gcgatatacg cagcgaattg agcggcataa cctgaatctg
    14401 aggcagcacc tggcacggct gggacggaag tcgctgtcgt tctcaaaatc ggtggagctg
    14461 catgacaaag tcatcgggca ttatctgaac ataaaacact atcaataagt tggagtcatt
    14521 acccaattat gatagaattt acaagctata aggttattgt cctgggtttc aagcattagt
    14581 ccatgcaagt ttttatgctt tgcccattct atagatatat tgataagcgc gctgcctatg
    14641 ccttgccccc tgaaatcctt acatacggcg atatcttcta tataaaagat atattatctt
    14701 atcagtattg tcaatatatt caaggcaatc tgcctcctca tcctcttcat cctcttcgtc
    14761 ttggtagctt tttaaatatg gcgcttcata gagtaattct gtaaaggtcc aattctcgtt
    14821 ttcatacctc ggtataatct tacctatcac ctcaaatggt tcgctgggtt tatcgcaccc
    14881 ccgaacacga gcacggcacc cgcgaccact atgccaagaa tgcccaaggt aaaaattgcc
    14941 ggccccgcca tgaagtccgt gaatgccccg acggccgaag tgaagggcag gccgccaccc
    15001 aggccgccgc cctcactgcc cggcacctgg tcgctgaatg tcgatgccag cacctgcggc
    15061 acgtcaatgc ttccgggcgt cgcgctcggg ctgatcgccc atcccgttac tgccccgatc
    15121 ccggcaatgg caaggactgc cagcgctgcc atttttgggg tgaggccgtt cgcggccgag
    15181 gggcgcagcc cctgggggga tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg
    15241 gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac
    15301 aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa
    15361 cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa
    15421 taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca
    15481 tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt
    15541 ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg
    15601 ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg
    15661 tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc
    15721 cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc
    15781 tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt
    15841 gagcgtcgga aaggcgc
//
