LOCUS       sequence-246342-       12878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 
            promoter in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12878)
  AUTHORS   Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC
  TITLE     Proximity labeling of protein complexes and cell type-specific 
            organellar proteomes in Arabidopsis enabled by TurboID.
  JOURNAL   Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864.
  PUBMED    31535972
REFERENCE   2  (bases 1 to 12878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-19"; volume: "8. pii: 47864. doi"; pages: " 
            10.7554/eLife.47864"
COMMENT     Sequence Label: R4pGWB601_UBQ10p-Turbo-NES-YFP
FEATURES             Location/Qualifiers
     source          1..12878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    269..289
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2308..2332
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2346..2387
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2394..3350
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             3357..3383
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(3414..3416,3417..3419,3420..4133)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3720..3739)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4067..4088
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4156..4180)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4212..4464
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4497..4513)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4497..4513)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4510..4532)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4521..4537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4545..4551,4552..4569,4570..4575))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4590..4611
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4647..4899)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5059..5610)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5662..5841)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6347..6371
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6892..7683
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7929..8517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8418..8437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8671..8688
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8703..8843
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8769..8788)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8929..8951
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9187..9381
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9447..10520)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10949..11578)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag cttgtggatc ccccatcaca actttgtata gaaaagttgg ctccgaattc
      301 gcccttagtc tagctcaaca gagcttttaa cccaaattgg tacaatagaa tacaacttta
      361 gatcataatt ctcaaaagaa agagattcct tagctattct atctgccact ccatttcctt
      421 ctcggcttgt atgcacaagc ataaaatcct caaacttgct aagtagatac tttatgtctt
      481 ggataattgg attgagactt gacaagcata actttcatgt aaccaaagac acaagttgct
      541 gagaatccac ctcaaaaatg atcttcctat aattgaatcg ggataatgac agcacagccc
      601 atctaagagc ctccacttct acttccagca cgcttcttac ttttaccaca gctcttgcac
      661 ctaaccataa caccttccct gtatgatcgc gaagcaccca ccctaagcca cattttaatc
      721 cttctgttgg ccatgcccca tcaaagttgc acttaaccca agattgtggt ggagcttccc
      781 atgtttctcg tctgtcccga cggtgttgtg gttggtgctt tccttacatt ctgagcctct
      841 ttccttctaa tccactcatc tgcatcttct tgtgtcctta ctaatacctc attggttcca
      901 aattccctcc ctttaagcac cagctcgttt ctgttcttcc acagcctccc aagtatccaa
      961 gggactaaag cctccacatt cttcagatca ggatattctt gtttaagatg ttgaactcta
     1021 tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc atagcgaact
     1081 tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa aaaatattat
     1141 tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa gatccattga
     1201 tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt ttttaacgag
     1261 acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa tcatacaaaa
     1321 atatccaata acactaaaaa attaaaagaa atggataatt tcacaatatg ttatacgata
     1381 aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat tagataaatg
     1441 gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa atacgaaata
     1501 cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc caccgtatat
     1561 ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac
     1621 ggctggatct tatgacgacc gttagaaatt gtggttgtcg acgagtcagt aataaacggc
     1681 gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag cacaaatact
     1741 tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta aacaacgctc
     1801 aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc tcgtgaccta
     1861 gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag ctcttcttct
     1921 tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg
     1981 tgatcaaggt aaatttctgt gttccttatt ctctcaaaat cttcgatttt gttttcgttc
     2041 gatcccaatt tcgtatatgt tctttggttt agattctgtt aatcttagat cgaagacgat
     2101 tttctgggtt tgatcgttag atatcatctt aattctcgat tagggtttca tagatatcat
     2161 ccgatttgtt caaataattt gagttttgtc gaataattac tcttcgattt gtgatttcta
     2221 tctagatctg gtgttagttt ctagtttgtg cgatcgaatt tgtcgattaa tctgagtttt
     2281 tctgattaac agaagggcga attcgaccca agtttgtaca aaaaagcagg ctccgcggcc
     2341 gcatgggcaa gcccatcccc aaccccctgc tgggcctgga cagcaccgct agcaaagaca
     2401 atactgtgcc tctgaagctg atcgctctcc tggctaatgg cgagttccat agtggcgaac
     2461 agctgggaga aaccctgggc atgtccaggg ccgctatcaa caagcacatt cagactctgc
     2521 gcgactgggg cgtggacgtg ttcaccgtgc ccggaaaggg ctactctctg cccgagccta
     2581 tcccgctgct gaacgctaaa cagattctgg gacagctgga cggcgggagc gtggcagtcc
     2641 tgcctgtggt cgactccacc aatcagtacc tgctggatcg aatcggcgag ctgaagagtg
     2701 gggatgcttg cattgcagaa tatcagcagg cagggagagg aagcagaggg aggaaatggt
     2761 tctctccttt tggagctaac ctgtacctga gtatgttttg gcgcctgaag cggggaccag
     2821 cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc agaagcgctg cgaaagctgg
     2881 gagcagacaa ggtgcgagtc aaatggccca atgacctgta tctgcaggat agaaagctgg
     2941 caggcatcct ggtggagctg gccggaataa caggcgatgc tgcacagatc gtcattggcg
     3001 ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt ggtcaatcag ggctggatca
     3061 cactgcagga agcagggatt aacctggaca ggaatactct ggccgctacg ctgatccgag
     3121 agctgcgggc agccctggaa ctgttcgagc aggaaggcct ggctccatat ctgccacggt
     3181 gggagaagct ggataacttc atcaatagac ccgtgaagct gatcattggg gacaaagaga
     3241 ttttcgggat tagccggggg attgataaac agggagccct gctgctggaa caggacggag
     3301 ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc tgccgaaaag ctgcagctgc
     3361 ctcccctgga gcgcctgacc ctggacggag gcggtggaag cggcggaggt tccatggtga
     3421 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3481 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3541 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3601 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     3661 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3721 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3781 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3841 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     3901 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     3961 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4021 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4081 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taagggccca
     4141 agggtgggcg cgccgaccca gctttcttgt acaaagtggt tgataacagc gcttagagct
     4201 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     4261 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     4321 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     4381 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     4441 cggtgtcatc tatgttacta gatcgggaat tggttccgga accaattcgt aatcatgtca
     4501 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4561 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aggctgaatt
     4621 aggcgcgcct atttctatta attcccgatc tagtaacata gatgacaccg cgcgcgataa
     4681 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
     4741 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
     4801 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt
     4861 caatcttaag aaactttatt gccaaatgtt tgaacgatcg gggaaattcg agctggggac
     4921 gtccccagct cggtaccccc tcctgggcac ggtcgtacgc gatccgccgc ggttccacct
     4981 caccattgcg ggtatgccag ttgagttcga tgagttcccg taccagctcc aactcgggcg
     5041 atgccacgcg cactccgttc agatctcggt gacgggcagg accggacggg gcggtaccgg
     5101 caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct tgaagccggc
     5161 cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca cgctcgggtc
     5221 gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca gggacttcag
     5281 caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg agacgtacac
     5341 ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc caggggcccg cgtaggcgat
     5401 gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc gcagacggac
     5461 gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga ccgtgcttgt
     5521 ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg tggcacggcg
     5581 gatgtcggcc gggcgtcgtt ctgggctcat atcttattgc cccccgggat cagatctgga
     5641 ttgagagtga atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga
     5701 gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc
     5761 aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg
     5821 gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc
     5881 ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcttgatc ccctgcgcca
     5941 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     6001 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag
     6061 ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct
     6121 tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc
     6181 tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga
     6241 agctgatccg tcgagattaa tagaaataaa ttcagcctaa ttcggcgtta attcagtaca
     6301 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     6361 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     6421 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     6481 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     6541 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     6601 ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     6661 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     6721 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     6781 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     6841 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa
     6901 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     6961 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     7021 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     7081 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     7141 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     7201 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     7261 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     7321 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     7381 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     7441 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     7501 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     7561 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     7621 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     7681 taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     7741 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     7801 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc
     7861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7981 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8041 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     8101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8281 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8521 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8581 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8641 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8701 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     8761 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     8821 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     8881 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     8941 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
     9001 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt
     9061 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
     9121 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
     9181 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
     9241 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
     9301 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
     9361 ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca
     9421 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
     9481 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
     9541 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
     9601 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
     9661 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
     9721 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
     9781 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
     9841 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
     9901 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
     9961 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
    10021 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
    10081 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
    10141 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
    10201 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
    10261 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
    10321 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
    10381 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
    10441 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
    10501 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
    10561 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
    10621 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
    10681 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
    10741 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
    10801 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
    10861 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
    10921 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
    10981 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
    11041 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
    11101 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
    11161 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
    11221 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
    11281 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
    11341 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
    11401 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
    11461 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
    11521 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
    11581 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
    11641 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
    11701 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
    11761 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
    11821 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
    11881 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
    11941 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
    12001 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
    12061 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
    12121 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
    12181 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
    12241 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
    12301 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
    12361 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
    12421 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
    12481 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
    12541 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
    12601 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
    12661 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
    12721 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa
    12781 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta
    12841 aatatccgat tattctaata aacgctcttt tctcttag
//
