LOCUS       sequence-246345-       12866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expressing nuclear TurboID-YFP under the UBQ10 
            promoter in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12866)
  AUTHORS   Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC
  TITLE     Proximity labeling of protein complexes and cell type-specific 
            organellar proteomes in Arabidopsis enabled by TurboID.
  JOURNAL   Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864.
  PUBMED    31535972
REFERENCE   2  (bases 1 to 12866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-19"; volume: "8. pii: 47864. doi"; pages: " 
            10.7554/eLife.47864"
COMMENT     Sequence Label: R4pGWB601_UBQ10p-Turbo-YFP-NLS
FEATURES             Location/Qualifiers
     source          1..12866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    269..289
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2308..2332
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2346..2387
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2394..3350
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             join(3378..3380,3381..3383,3384..4094)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3684..3703)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4031..4052
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4104..4124
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(4144..4168)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4200..4452
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4485..4501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4485..4501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4498..4520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4509..4525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4533..4539,4540..4557,4558..4563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4578..4599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4635..4887)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5047..5598)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5650..5829)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6335..6359
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6880..7671
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7917..8505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8406..8425
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8659..8676
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8691..8831
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8757..8776)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8917..8939
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9175..9369
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9435..10508)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10937..11566)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag cttgtggatc ccccatcaca actttgtata gaaaagttgg ctccgaattc
      301 gcccttagtc tagctcaaca gagcttttaa cccaaattgg tacaatagaa tacaacttta
      361 gatcataatt ctcaaaagaa agagattcct tagctattct atctgccact ccatttcctt
      421 ctcggcttgt atgcacaagc ataaaatcct caaacttgct aagtagatac tttatgtctt
      481 ggataattgg attgagactt gacaagcata actttcatgt aaccaaagac acaagttgct
      541 gagaatccac ctcaaaaatg atcttcctat aattgaatcg ggataatgac agcacagccc
      601 atctaagagc ctccacttct acttccagca cgcttcttac ttttaccaca gctcttgcac
      661 ctaaccataa caccttccct gtatgatcgc gaagcaccca ccctaagcca cattttaatc
      721 cttctgttgg ccatgcccca tcaaagttgc acttaaccca agattgtggt ggagcttccc
      781 atgtttctcg tctgtcccga cggtgttgtg gttggtgctt tccttacatt ctgagcctct
      841 ttccttctaa tccactcatc tgcatcttct tgtgtcctta ctaatacctc attggttcca
      901 aattccctcc ctttaagcac cagctcgttt ctgttcttcc acagcctccc aagtatccaa
      961 gggactaaag cctccacatt cttcagatca ggatattctt gtttaagatg ttgaactcta
     1021 tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc atagcgaact
     1081 tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa aaaatattat
     1141 tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa gatccattga
     1201 tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt ttttaacgag
     1261 acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa tcatacaaaa
     1321 atatccaata acactaaaaa attaaaagaa atggataatt tcacaatatg ttatacgata
     1381 aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat tagataaatg
     1441 gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa atacgaaata
     1501 cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc caccgtatat
     1561 ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac
     1621 ggctggatct tatgacgacc gttagaaatt gtggttgtcg acgagtcagt aataaacggc
     1681 gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag cacaaatact
     1741 tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta aacaacgctc
     1801 aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc tcgtgaccta
     1861 gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag ctcttcttct
     1921 tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg
     1981 tgatcaaggt aaatttctgt gttccttatt ctctcaaaat cttcgatttt gttttcgttc
     2041 gatcccaatt tcgtatatgt tctttggttt agattctgtt aatcttagat cgaagacgat
     2101 tttctgggtt tgatcgttag atatcatctt aattctcgat tagggtttca tagatatcat
     2161 ccgatttgtt caaataattt gagttttgtc gaataattac tcttcgattt gtgatttcta
     2221 tctagatctg gtgttagttt ctagtttgtg cgatcgaatt tgtcgattaa tctgagtttt
     2281 tctgattaac agaagggcga attcgaccca agtttgtaca aaaaagcagg ctccgcggcc
     2341 gcatgggcaa gcccatcccc aaccccctgc tgggcctgga cagcaccgct agcaaagaca
     2401 atactgtgcc tctgaagctg atcgctctcc tggctaatgg cgagttccat agtggcgaac
     2461 agctgggaga aaccctgggc atgtccaggg ccgctatcaa caagcacatt cagactctgc
     2521 gcgactgggg cgtggacgtg ttcaccgtgc ccggaaaggg ctactctctg cccgagccta
     2581 tcccgctgct gaacgctaaa cagattctgg gacagctgga cggcgggagc gtggcagtcc
     2641 tgcctgtggt cgactccacc aatcagtacc tgctggatcg aatcggcgag ctgaagagtg
     2701 gggatgcttg cattgcagaa tatcagcagg cagggagagg aagcagaggg aggaaatggt
     2761 tctctccttt tggagctaac ctgtacctga gtatgttttg gcgcctgaag cggggaccag
     2821 cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc agaagcgctg cgaaagctgg
     2881 gagcagacaa ggtgcgagtc aaatggccca atgacctgta tctgcaggat agaaagctgg
     2941 caggcatcct ggtggagctg gccggaataa caggcgatgc tgcacagatc gtcattggcg
     3001 ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt ggtcaatcag ggctggatca
     3061 cactgcagga agcagggatt aacctggaca ggaatactct ggccgctacg ctgatccgag
     3121 agctgcgggc agccctggaa ctgttcgagc aggaaggcct ggctccatat ctgccacggt
     3181 gggagaagct ggataacttc atcaatagac ccgtgaagct gatcattggg gacaaagaga
     3241 ttttcgggat tagccggggg attgataaac agggagccct gctgctggaa caggacggag
     3301 ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc tgccgaaaag ggaggcggtg
     3361 gaagcggcgg aggttccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3421 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     3481 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     3541 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct
     3601 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     3661 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     3721 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     3781 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     3841 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     3901 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     3961 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
     4021 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4081 acgagctgta caaggcttcg gatccaaaaa agaagagaaa ggtatagaag ggtgggcgcg
     4141 ccgacccagc tttcttgtac aaagtggttg ataacagcgc ttagagctcg aatttccccg
     4201 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     4261 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     4321 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     4381 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     4441 tgttactaga tcgggaattg gttccggaac caattcgtaa tcatgtcata gctgtttcct
     4501 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4561 aaagcctggg gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat
     4621 ttctattaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
     4681 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
     4741 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
     4801 attcaacaga aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa
     4861 actttattgc caaatgtttg aacgatcggg gaaattcgag ctggggacgt ccccagctcg
     4921 gtaccccctc ctgggcacgg tcgtacgcga tccgccgcgg ttccacctca ccattgcggg
     4981 tatgccagtt gagttcgatg agttcccgta ccagctccaa ctcgggcgat gccacgcgca
     5041 ctccgttcag atctcggtga cgggcaggac cggacggggc ggtaccggca ggctgaagtc
     5101 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
     5161 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
     5221 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
     5281 gagcgtggag cccagtcccg tccgctggtg gcggggggag acgtacacgg tcgactcggc
     5341 cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg taggcgatgc cggcgacctc
     5401 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
     5461 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
     5521 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
     5581 gcgtcgttct gggctcatat cttattgccc cccgggatca gatctggatt gagagtgaat
     5641 atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca
     5701 agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc
     5761 tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg
     5821 ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat
     5881 aacgtgactc ccttaattct ccgctcatga tcttgatccc ctgcgccatc agatccttgg
     5941 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     6001 agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt
     6061 aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc
     6121 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt
     6181 ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctgatccgtc
     6241 gagattaata gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc
     6301 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     6361 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     6421 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     6481 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     6541 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt
     6601 cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     6661 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     6721 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     6781 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     6841 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc
     6901 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     6961 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     7021 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     7081 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     7141 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     7201 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     7261 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     7321 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     7381 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     7441 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     7501 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     7561 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     7621 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct
     7681 agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     7741 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     7801 cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa
     7861 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7921 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7981 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8041 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     8101 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8161 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8221 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8281 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8341 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8401 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8461 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8521 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8581 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8641 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     8701 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     8761 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     8821 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8881 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8941 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     9001 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     9061 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     9121 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     9181 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     9241 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     9301 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     9361 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     9421 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     9481 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     9541 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     9601 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     9661 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     9721 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     9781 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     9841 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     9901 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     9961 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
    10021 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
    10081 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
    10141 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
    10201 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
    10261 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
    10321 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
    10381 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
    10441 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
    10501 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
    10561 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
    10621 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
    10681 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
    10741 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
    10801 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
    10861 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
    10921 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
    10981 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
    11041 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
    11101 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
    11161 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
    11221 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
    11281 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    11341 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    11401 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    11461 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    11521 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    11581 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    11641 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    11701 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    11761 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    11821 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    11881 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    11941 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    12001 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    12061 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    12121 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    12181 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    12241 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    12301 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    12361 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    12421 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    12481 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    12541 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    12601 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    12661 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    12721 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    12781 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta
    12841 ttctaataaa cgctcttttc tcttag
//
