LOCUS       sequence-24635-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  TITLE     Lowe Lab Plasmids
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe H-Ras V12 C40
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(13..45,46..48,49..582)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSCRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     primer_bind     complement(601..621)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     gap             626..637
                     /estimated_length=12
ORIGIN
        1 gattcntcga ccatgacgga atataagctg gtggtggtgg gcgccgtcgg tgtgggcaag
       61 agtgcgctga ccatccagct gatccagaac cattttgtgg acgaatacga ccccactata
      121 gaggattcct gccggaagca ggtggtcatt gatggggaga cgtgcctgtt ggacatcctg
      181 gataccgccg gccaggagga gtacagcgcc atgcgggacc agtacatgcg caccggggag
      241 ggcttcctgt gtgtgtttgc catcaacaac accaagtctt ttgaggacat ccaccagtac
      301 agggagcaga tcaaacgggt gaaggactcg gatgacgtgc ccatggtgct ggtggggaac
      361 aagtgtgacc tggctgcacg cactgtggaa tctcggcagg ctcaggacct cgcccgaagc
      421 tacggcatcc cctacatcga gacctcggcc aagacccggc agggagtgga ggatgccttc
      481 tacacgttgg tgcgtgagat ccggcagcac aagctgcgga agctgaaccc tcctgatgag
      541 agtggccccg gctgcatgag ctgcaagtgt gtgctctcct gaggatctag tcgaccctgt
      601 ggaatgtgtg tcagttaggg tgtgannnnn nnnnnnnccc cagncaggca nnnnnatgca
      661 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccngctc cccagcangc
      721 agaagtatgc aaagcatgca tctcattann nnncaaccat agtcccgccc ctaactccgc
      781 ccatcccgcc cctaactccg cccagttccg cccattctc
//
