LOCUS       sequence-246351-       12680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 
            promoter in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12680)
  AUTHORS   Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC
  TITLE     Proximity labeling of protein complexes and cell type-specific 
            organellar proteomes in Arabidopsis enabled by TurboID.
  JOURNAL   Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864.
  PUBMED    31535972
REFERENCE   2  (bases 1 to 12680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-19"; volume: "8. pii: 47864. doi"; pages: " 
            10.7554/eLife.47864"
COMMENT     Sequence Label: R4pGWB601_UBQ10p-miniTurbo-YFP-NLS
FEATURES             Location/Qualifiers
     source          1..12680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..210
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     202..219
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     203..219
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    244..264
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2283..2307
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2321..2362
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2369..3139
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             join(3167..3169,3170..3172,3173..3883)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3473..3492)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3820..3841
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3893..3913
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3933..3957)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3989..4241
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4274..4290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4274..4290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4287..4309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4298..4314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4322..4328,4329..4346,4347..4352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4367..4388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4424..4676)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4836..5387)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5439..5618)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6124..6148
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6669..7460
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7706..8294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8195..8214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8448..8465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8480..8620
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8546..8565)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8706..8728
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8964..9158
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9224..10297)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10726..11355)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12656..12680
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aacactgata gtttaaactg aaggcgggaa acgacaatct gatccaagct caagctgctc
       61 tagcattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      121 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      181 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttgt ggatccccca
      241 tcacaacttt gtatagaaaa gttggctccg aattcgccct tagtctagct caacagagct
      301 tttaacccaa attggtacaa tagaatacaa ctttagatca taattctcaa aagaaagaga
      361 ttccttagct attctatctg ccactccatt tccttctcgg cttgtatgca caagcataaa
      421 atcctcaaac ttgctaagta gatactttat gtcttggata attggattga gacttgacaa
      481 gcataacttt catgtaacca aagacacaag ttgctgagaa tccacctcaa aaatgatctt
      541 cctataattg aatcgggata atgacagcac agcccatcta agagcctcca cttctacttc
      601 cagcacgctt cttactttta ccacagctct tgcacctaac cataacacct tccctgtatg
      661 atcgcgaagc acccacccta agccacattt taatccttct gttggccatg ccccatcaaa
      721 gttgcactta acccaagatt gtggtggagc ttcccatgtt tctcgtctgt cccgacggtg
      781 ttgtggttgg tgctttcctt acattctgag cctctttcct tctaatccac tcatctgcat
      841 cttcttgtgt ccttactaat acctcattgg ttccaaattc cctcccttta agcaccagct
      901 cgtttctgtt cttccacagc ctcccaagta tccaagggac taaagcctcc acattcttca
      961 gatcaggata ttcttgttta agatgttgaa ctctatggag gtttgtatga actgatgatc
     1021 taggaccgga taagttccct tcttcatagc gaacttattc aaagaatgtt ttgtgtatca
     1081 ttcttgttac attgttatta atgaaaaaat attattggtc attggactga acacgagtgt
     1141 taaatatgga ccaggcccca aataagatcc attgatatat gaattaaata acaagaataa
     1201 atcgagtcac caaaccactt gcctttttta acgagacttg ttcaccaact tgatacaaaa
     1261 gtcattatcc tatgcaaatc aataatcata caaaaatatc caataacact aaaaaattaa
     1321 aagaaatgga taatttcaca atatgttata cgataaagaa gttacttttc caagaaattc
     1381 actgatttta taagcccact tgcattagat aaatggcaaa aaaaaacaaa aaggaaaaga
     1441 aataaagcac gaagaattct agaaaatacg aaatacgctt caatgcagtg ggacccacgg
     1501 ttcaattatt gccaattttc agctccaccg tatatttaaa aaataaaacg ataatgctaa
     1561 aaaaatataa atcgtaacga tcgttaaatc tcaacggctg gatcttatga cgaccgttag
     1621 aaattgtggt tgtcgacgag tcagtaataa acggcgtcaa agtggttgca gccggcacac
     1681 acgagtcgtg tttatcaact caaagcacaa atacttttcc tcaacctaaa aataaggcaa
     1741 ttagccaaaa acaactttgc gtgtaaacaa cgctcaatac acgtgtcatt ttattattag
     1801 ctattgcttc accgccttag ctttctcgtg acctagtcgt cctcgtcttt tcttcttctt
     1861 cttctataaa acaataccca aagagctctt cttcttcaca attcagattt caatttctca
     1921 aaatcttaaa aactttctct caattctctc taccgtgatc aaggtaaatt tctgtgttcc
     1981 ttattctctc aaaatcttcg attttgtttt cgttcgatcc caatttcgta tatgttcttt
     2041 ggtttagatt ctgttaatct tagatcgaag acgattttct gggtttgatc gttagatatc
     2101 atcttaattc tcgattaggg tttcatagat atcatccgat ttgttcaaat aatttgagtt
     2161 ttgtcgaata attactcttc gatttgtgat ttctatctag atctggtgtt agtttctagt
     2221 ttgtgcgatc gaatttgtcg attaatctga gtttttctga ttaacagaag ggcgaattcg
     2281 acccaagttt gtacaaaaaa gcaggctccg cggccgcatg ggcaagccca tccccaaccc
     2341 cctgctgggc ctggacagca ccgctagcat cccgctgctg aacgctaaac agattctggg
     2401 acagctggac ggcgggagcg tggcagtcct gcctgtggtc gactccacca atcagtacct
     2461 gctggatcga atcggcgagc tgaagagtgg ggatgcttgc attgcagaat atcagcaggc
     2521 agggagagga agcagaggga ggaaatggtt ctctcctttt ggagctaacc tgtacctgag
     2581 tatgttttgg cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg tcatcggaat
     2641 tgtcatggca gaagcgctgc gaaagctggg agcagacaag gtgcgagtca aatggcccaa
     2701 tgacctgtat ctgcaggata gaaagctggc aggcatcctg gtggagctgg ccggaataac
     2761 aggcgatgct gcacagatcg tcattggcgc cgggattaac gtggctatga ggcgcgtgga
     2821 ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa gcagggatta acctggacag
     2881 gaatactctg gccgctatgc tgatccgaga gctgcgggca gccctggaac tgttcgagca
     2941 ggaaggcctg gctccatatc tgtcacggtg ggagaagctg gataacttca tcaatagacc
     3001 cgtgaagctg atcattgggg acaaagagat tttcgggatt agccggggga ttgataaaca
     3061 gggagccctg ctgctggaac aggacggagt tatcaaaccc tggatgggcg gagaaatcag
     3121 tctgcggtct gccgaaaagg gaggcggtgg aagcggcgga ggttccatgg tgagcaaggg
     3181 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     3241 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     3301 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     3361 cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     3421 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     3481 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3541 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3601 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3661 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3721 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     3781 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3841 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggcttcgg atccaaaaaa
     3901 gaagagaaag gtatagaagg gtgggcgcgc cgacccagct ttcttgtaca aagtggttga
     3961 taacagcgct tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     4021 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     4081 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     4141 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     4201 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattgg ttccggaacc
     4261 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4321 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4381 tcacattagg ctgaattagg cgcgcctatt tctattaatt cccgatctag taacatagat
     4441 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
     4501 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
     4561 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
     4621 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg
     4681 aaattcgagc tggggacgtc cccagctcgg taccccctcc tgggcacggt cgtacgcgat
     4741 ccgccgcggt tccacctcac cattgcgggt atgccagttg agttcgatga gttcccgtac
     4801 cagctccaac tcgggcgatg ccacgcgcac tccgttcaga tctcggtgac gggcaggacc
     4861 ggacggggcg gtaccggcag gctgaagtcc agctgccaga aacccacgtc atgccagttc
     4921 ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg catatccgag cgcctcgtgc
     4981 atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga ccacgctctt gaagccctgt
     5041 gcctccaggg acttcagcag gtgggtgtag agcgtggagc ccagtcccgt ccgctggtgg
     5101 cggggggaga cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg tgccttccag
     5161 gggcccgcgt aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag ccagggatag
     5221 cgctcccgca gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg ctcggtacgg
     5281 aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc cggcatgtcc
     5341 gcctcggtgg cacggcggat gtcggccggg cgtcgttctg ggctcatatc ttattgcccc
     5401 ccgggatcag atctggattg agagtgaata tgagactcta attggatacc gaggggaatt
     5461 tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag
     5521 gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag
     5581 aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg
     5641 gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat
     5701 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
     5761 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     5821 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     5881 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     5941 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag
     6001 tgcttgcggc agcgtgaagc tgatccgtcg agattaatag aaataaattc agcctaattc
     6061 ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
     6121 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
     6181 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
     6241 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
     6301 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
     6361 tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt
     6421 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     6481 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     6541 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     6601 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     6661 taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     6721 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     6781 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     6841 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     6901 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     6961 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     7021 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     7081 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     7141 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     7201 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     7261 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     7321 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     7381 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     7441 ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg
     7501 gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc
     7561 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
     7621 atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7681 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8281 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8341 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8401 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8461 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     8521 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     8581 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     8641 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     8701 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     8761 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg
     8821 gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac
     8881 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt
     8941 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa
     9001 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca
     9061 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa
     9121 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt
     9181 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg
     9241 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct
     9301 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac
     9361 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc
     9421 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg
     9481 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga
     9541 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc
     9601 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc
     9661 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg
     9721 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt
     9781 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt
     9841 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg
     9901 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca
     9961 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc
    10021 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc
    10081 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc
    10141 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt
    10201 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc
    10261 cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt
    10321 tccagtgcca ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt
    10381 tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc
    10441 gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta
    10501 gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca
    10561 tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg
    10621 tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac
    10681 ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga
    10741 tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc
    10801 aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga
    10861 ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt
    10921 gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat
    10981 gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg
    11041 caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg
    11101 gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat
    11161 cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc
    11221 gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag
    11281 ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac
    11341 agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac
    11401 gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg
    11461 cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa
    11521 ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac
    11581 ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg
    11641 tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt
    11701 cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca
    11761 cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag
    11821 ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg
    11881 ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc
    11941 cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg
    12001 tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca
    12061 ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg
    12121 ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc
    12181 tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc
    12241 agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc
    12301 gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct
    12361 agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg
    12421 gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg
    12481 gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac
    12541 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
    12601 cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta
    12661 cccgccaata tatcctgtca
//
