LOCUS       sequence-246352-        1895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human pro-TGF-beta-2 (from L21 to S414) in insect cells. 
            With C-t H6x tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Marino-Puertas L, Del Amo-Maestro L, Taules M, Gomis-Ruth FX, Goulas
            T
  TITLE     Recombinant production of human alpha2-macroglobulin variants and 
            interaction studies with recombinant G-related alpha2-macroglobulin 
            binding protein and latent transforming growth factor-beta2.
  JOURNAL   Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.1038/s41598-019-45712-z.
  PUBMED    31235767
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-06-24"; volume: "9(1):9186. doi"; pages: " 
            10.1038/s41598-019-45712-z"
COMMENT     Sequence Label: pIE-TGFB2-H6
FEATURES             Location/Qualifiers
     source          1..1895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..592
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             1843..1860
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgatgtcttt gtgatgcgcg cgacattttt gtaggttatt gataaaatga acggatacgt
       61 tgcccgacat tatcattaaa tccttggcgt agaatttgtc gggtccattg tccgtgtgcg
      121 ctagcatgcc cgtaacggac ctcgtacttt tggcttcaaa ggttttgcgc acagacaaaa
      181 tgtgccacac ttgcagctct gcatgtgtgc gcgttaccac aaatcccaac ggcgcagtgt
      241 acttgttgta tgcaaataaa tctcgataaa ggcgcggcgc gcgaatgcag ctgatcacgt
      301 acgctcctcg tgttccgttc aaggacggtg ttatcgacct cagattaatg tttatcggcc
      361 gactgttttc gtatccgctc accaaacgcg tttttgcatt aacattgtat gtcggcggat
      421 gttctatatc taatttgaat aaataaacga taaccgcgtt ggttttagag ggcataataa
      481 aagaaatatt gttatcgtgt tcgccattag ggcagtataa attgacgttc atgttggata
      541 ttgtttcagt tgcaagttga cactggcggc gacaagatcg tgaacaacca agtgactatg
      601 tacaagctca cagtcttcct gatgttcatc gctttcgtca tcatcgctga ggccttgtct
      661 acctgcagca cactcgatat ggaccagttc atgcgcaaga ggatcgaggc gatccgcggg
      721 cagatcctga gcaagctgaa gctcaccagt cccccagaag actatcctga gcccgaggaa
      781 gtccccccgg aggtgatttc catctacaac agcaccaggg acttgctcca ggagaaggcg
      841 agccggaggg cggccgcctg cgagcgcgag aggagcgacg aagagtacta cgccaaggag
      901 gtttacaaaa tagacatgcc gcccttcttc ccctccgaaa atgccatccc gcccactttc
      961 tacagaccct acttcagaat tgttcgattt gacgtctcag caatggagaa gaatgcttcc
     1021 aatttggtga aagcagagtt cagagtcttt cgtttgcaga acccaaaagc cagagtgcct
     1081 gaacaacgga ttgagctata tcagattctc aagtccaaag atttaacatc tccaacccag
     1141 cgctacatcg acagcaaagt tgtgaaaaca agagcagaag gcgaatggct ctccttcgat
     1201 gtaactgatg ctgttcatga atggcttcac cataaagaca ggaacctggg atttaaaata
     1261 agcttacact gtccctgctg cacttttgta ccatctaata attacatcat cccaaataaa
     1321 agtgaagaac tagaagcaag atttgcaggt attgatggca cctccacata taccagtggt
     1381 gatcagaaaa ctataaagtc cactaggaaa aaaaacagtg ggaagacccc acatctcctg
     1441 ctaatgttat tgccctccta cagacttgag tcacaacaga ccaaccggcg gaagaagcgt
     1501 gctttggatg cggcctattg ctttagaaat gtgcaggata attgctgcct acgtccactt
     1561 tacattgatt tcaagaggga tctagggtgg aaatggatac acgaacccaa agggtacaat
     1621 gccaacttct gtgctggagc atgcccgtat ttatggagtt cagacactca gcacagcagg
     1681 gtcctgagct tatataatac cataaatcca gaagcatctg cttctccttg ctgcgtgtcc
     1741 caagatttag aacctctaac cattctctac tacattggca aaacacccaa gattgaacag
     1801 ctttctaata tgattgtaaa gtcttgcaaa tgcagcaccg gtcatcatca ccatcaccat
     1861 tgagtttggc aaggccggat ccgttctacc ctgcg
//
