LOCUS       sequence-24636-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  TITLE     Lowe Lab Plasmids
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWZL Hygro
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    84..545
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(140..157)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     367..386
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tgtggtggta cgtaggaatt cgccagcaca gtggtcgacc aattccggtt attttccacc
       61 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
      121 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
      181 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
      241 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
      301 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
      361 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
      421 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
      481 ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
      541 gataatacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
      601 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
      661 agcttcgatg tangagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
      721 tacaaagatc gttatgttta tcggcacttn gcatcggccg cgctcccgat tcc
//
