LOCUS       sequence-246381-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses DsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Wang X, Cato P, Lin HC, Li T, Wan D, Alcocer MJ, Falcone FH
  TITLE     Optimisation and use of humanised RBL NF-AT-GFP and NF-AT-DsRed 
            reporter cell lines suitable for high-throughput scale detection of 
            allergic sensitisation in array format and identification of the 
            ECM-integrin interaction as critical factor.
  JOURNAL   Mol Biotechnol. 2014 Feb;56(2):136-46. doi: 
            10.1007/s12033-013-9689-x.
  PUBMED    23893250
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biotechnol"; date: "2014-02"; volume: "56"; issue: "2"; pages: 
            "136-46"
COMMENT     Sequence Label: pMJA167
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..678
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(181..201)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     399..418
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     593..616
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atggatagca ctgagaacgt catcaagccc ttcatgcgct tcaaggtgca catggagggc
       61 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggcaagcc ctacgagggc
      121 acccagaccg ccaagctgca ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      181 ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc
      241 gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      301 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcac cttcatctac
      361 cacgtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtaatgca gaagaagact
      421 ctgggctggg agccctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
      481 atccacaagg cgctgaagct gaagggcggc ggccactacc tggtggagtt caagtcaatc
      541 tacatggcca agaagcccgt gaagctgccc ggctactact acgtggactc caagctggac
      601 atcacctccc acaacgagga ctacaccgtg gtggagcagt acgagcgcgc cgaggcccgc
      661 caccacctgt tccagtag
//
