LOCUS       sequence-246383-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E coli Bir A protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   de la Cruz S, Alcocer M, Madrid R, Garcia A, Martin R, Gonzalez I, 
            Garcia T
  TITLE     Production of in vivo biotinylated scFv specific to almond (Prunus 
            dulcis) proteins by recombinant Pichia pastoris.
  JOURNAL   J Biotechnol. 2016 Jun 10;227:112-9. doi: 
            10.1016/j.jbiotec.2016.04.024. Epub 2016 Apr 13.
  PUBMED    27085890
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2016-06-10"; volume: "227:112-9. doi"; pages: "
            10.1016/j.jbiotec.2016.04.024. Epub 2016 Apr 13"
COMMENT     Sequence Label: pMJA180
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..966
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1..963
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
ORIGIN
        1 atgaaggata acaccgtgcc actgaaattg attgccctgt tagcgaacgg tgaatttcac
       61 tctggcgagc agttgggtga aacgctggga atgagccggg cggctattaa taaacacatt
      121 cagacactgc gtgactgggg cgttgatgtc tttaccgttc cgggtaaagg atacagcctg
      181 cctgagccta tccagttact taatgctaaa cagatattgg gtcagctgga tggcggtagt
      241 gtagccgtgc tgccagtgat tgactccacg aatcagtacc ttcttgatcg tatcggagag
      301 cttaaatcgg gcgatgcttg cattgcagaa taccagcagg ctggccgtgg tcgccggggt
      361 cggaaatggt tttcgccttt tggcgcaaac ttatatttgt cgatgttctg gcgtctggaa
      421 caaggcccgg cggcggcgat tggtttaagt ctggttatcg gtatcgtgat ggcggaagta
      481 ttacgcaagc tgggtgcaga taaagttcgt gttaaatggc ctaatgacct ctatctgcag
      541 gatcgcaagc tggcaggcat tctggtggag ctgactggca aaactggcga tgcggcgcaa
      601 atagtcattg gagccgggat caacatggca atgcgccgtg ttgaagagag tgtcgttaat
      661 caggggtgga tcacgctgca ggaagcgggg atcaatctcg atcgtaatac gttggcggcc
      721 atgctaatac gtgaattacg tgctgcgttg gaactcttcg aacaagaagg attggcacct
      781 tatctgtcgc gctgggaaaa gctggataat tttattaatc gcccagtgaa acttatcatt
      841 ggtgataaag aaatatttgg catttcacgc ggaatagaca aacagggggc tttattactt
      901 gagcaggatg gaataataaa accctggatg ggcggtgaaa tatccctgcg tagtgcagaa
      961 aaataa
//
