LOCUS       sequence-246401-       13216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Cribbs LL, Lee JH, Yang J, Satin J, Zhang Y, Daud A, Barclay J, 
            Williamson MP, Fox M, Rees M, Perez-Reyes E
  TITLE     Cloning and characterization of alpha1H from human heart, a member 
            of the T-type Ca2+ channel gene family.
  JOURNAL   Circ Res. 1998 Jul 13;83(1):103-9.
  PUBMED    9670923
REFERENCE   2  (bases 1 to 13216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res.";
            date: "1998-07-13"; volume: "83(1)"; pages: "103-9"
COMMENT     Sequence Label: a1Ha-pcDNA3
FEATURES             Location/Qualifiers
     source          1..13216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2602..2628
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     promoter        complement(8769..8787)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8770..8787)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8807..8824)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8813..9037
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9083..9511
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9170..9189)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9380..9401
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9520..9540)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9525..9854
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9705..9840
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9921..10715
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9975..9994)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10585..10604
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10889..11010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10926..10945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10980..10999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11059..11075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11059..11075)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11072..11094)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11083..11099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11107..11113,11114..11131,11132..11137))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11152..11173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11290..11307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11461..12049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11541..11560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12220..13011,13012..13080))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12843..12862
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13081..13185)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atcatcgatg
      961 aattccgggc ggtgaccgcg ccgcccgggc gatgcccgcg gggacgccgc cggccagcag
     1021 agcgaggtgc tgccggccgc caccatgacc gagggcgcac gggccgccga cgaggtccgg
     1081 gtgcccctgg gcgcgccgcc ccctggccct gcggcgttgg tgggggcgtc cccggagagc
     1141 cccggggcgc cgggacgcga ggcggagcgg gggtccgagc tcggcgtgtc accctccgag
     1201 agcccggcgg ccgagcgcgg cgcggagctg ggtgccgacg aggagcagcg cgtcccgtac
     1261 ccggccttgg cggccacggt cttcttctgc ctcggtcaga ccacgcggcc gcgcagctgg
     1321 tgcctccggc tggtctgcaa cccatggttc gagcacgtga gcatgctggt aatcatgctc
     1381 aactgcgtga ccctgggcat gttccggccc tgtgaggacg ttgagtgcgg ctccgagcgc
     1441 tgcaacatcc tggaggcctt tgacgccttc attttcgcct tttttgcggt ggagatggtc
     1501 atcaagatgg tggccttggg gctgttcggg cagaagtgtt acctgggtga cacgtggaac
     1561 aggctggatt tcttcatcgt cgtggcgggc atgatggagt actcgttgga cggacacaac
     1621 gtgagcctct cggctatcag gaccgtgcgg gtgctgcggc ccctccgcgc catcaaccgc
     1681 gtgcctagca tgcggatcct ggtcactctg ctgctggata cgctgcccat gctcgggaac
     1741 gtccttctgc tgtgcttctt cgtcttcttc attttcggca tcgttggcgt ccagctctgg
     1801 gctggcctcc tgcggaaccg ctgcttcctg gacagtgcct ttgtcaggaa caacaacctg
     1861 accttcctgc ggccgtacta ccagacggag gagggcgagg agaacccgtt catctgctcc
     1921 tcacgccgag acaacggcat gcagaagtgc tcgcacatcc ccggccgccg cgagctgcgc
     1981 atgccctgca ccctgggctg ggaggcctac acgcagccgc aggccgaggg ggtgggcgct
     2041 gcacgcaacg cctgcatcaa ctggaaccag tactacaacg tgtgccgctc gggtgactcc
     2101 aacccccaca acggtgccat caacttcgac aacatcggct acgcctggat cgccatcttc
     2161 caggtgatca cgctggaagg ctgggtggac atcatgtact acgtcatgga cgcccactca
     2221 ttctacaact tcatctattt catcctgctc atcatcgtgg gctccttctt catgatcaac
     2281 ctgtgcctgg tggtgattgc cacgcagttc tcggagacga agcagcggga gagtcagctg
     2341 atgcgggagc agcgggcacg ccacctgtcc aacgacagca cgctggccag cttctccgag
     2401 cctggcagct gctacgaaga gctgctgaag tacgtgggcc acatattccg caaggtcaag
     2461 cggcgcagct tgcgcctcta cgcccgctgg cagagccgct ggcgcaagaa ggtggacccc
     2521 agtgctgtgc aaggccaggg tcccgggcac cgccagcgcc gggcaggcag gcacacagcc
     2581 tcggtgcacc acctggtcta ccaccaccat caccaccacc accaccacta ccatttcagc
     2641 catggcagcc cccgcaggcc cggccccgag ccaggcgcct gcgacaccag gctggtccga
     2701 gctggcgcgc ccccctcgcc accttcccca ggccgcggac cccccgacgc agagtctgtg
     2761 cacagcatct accatgccga ctgccacata gaggggccgc aggagagggc ccgggtggca
     2821 catgccgcag ccactgctgc tgccagcctc aggctggcca cagggctggg caccatgaac
     2881 taccccacga tcctgccctc aggggtgggc agcggcaaag gcagcaccag ccccggaccc
     2941 aaggggaagt gggccggtgg accgccaggc accggggggc acggcccgtt gagcttgaac
     3001 agccctgatc cctacgagaa gatcccgcat gtggccgggg agcatggact gggccaagcc
     3061 cctggccatc tgtcgggcct cagtgtgccc tgccccctgc ccagcccccc agcgggcaca
     3121 ctgacctgtg agctgaagag ctgcccgtac tgcacccgtg ccctggagga cccggagggt
     3181 gagctcagcg gctcggaaag tggagactca gatggccgtg gcgtctatga attcacgcag
     3241 gacgtccggc acggtgaccg ctgggacccc acgcgaccac cccgtgcgac ggacacacca
     3301 ggcccaggcc caggcagccc ccagcggcgg gcacagcaga gggcagcccc gggcgagcca
     3361 ggctggatgg gccgcctctg ggttaccttc agcggcaagc tgcgccgcat cgtggacagc
     3421 aagtacttca gccgtggcat catgatggcc atccttgtca acacgctgag catgggcgtg
     3481 gagtaccatg agcagcccga ggagctgact aatgctctgg agatcagcaa catcgtgttc
     3541 accagcatgt ttgccctgga gatgctgctg aagctgctgg cctgcggccc tctgggctac
     3601 atccggaacc cgtacaacat cttcgacggc atcatcgtgg tcatcagcgt ctgggagatc
     3661 gtggggcagg cggacggtgg cttgtctgtg ctgcgcacct tccggctgct gcgtgtgctg
     3721 aagctggtgc gctttctgcc agccctgcgg cgccagctcg tggtgctggt gaagaccatg
     3781 gacaacgtgg ctaccttctg cacgctgctc atgctcttca ttttcatctt cagcatcctg
     3841 ggcatgcacc ttttcggctg caagttcagc ctgaagacag acaccggaga caccgtgcct
     3901 gacaggaaga acttcgactc cctgctgtgg gccatcgtca ccgtgttcca gatcctgacc
     3961 caggaggact ggaacgtggt cctgtacaac ggcatggcct ccacctcctc ctgggccgcc
     4021 ctctacttcg tggccctcat gaccttcggc aactatgtgc tcttcaacct gctggtggcc
     4081 atcctcgtgg agggcttcca ggcggagggc gatgccaaca gatccgacac ggacgaggac
     4141 aagacgtcgg tccacttcga ggaggacttc cacaagctca gagaactcca gaccacagag
     4201 ctgaagatgt gttccctggc cgtgaccccc aacgggcacc tggagggacg aggcagcctg
     4261 tcccctcccc tcatcatgtg cacagctgcc acgcccatgc ctacccccaa gagctcacca
     4321 ttcctggatg cagcccccag cctcccagac tctcggcgtg gcagcagcag ctccggggac
     4381 ccgccactgg gagaccagaa gcctccggcc agcctccgaa gttctccctg tgccccctgg
     4441 ggccccagtg gcgcctggag cagccggcgc tccagctgga gcagcctggg ccgtgccccc
     4501 agcctcaagc gccgcggcca gtgtggggaa cgtgagtccc tgctgtctgg cgagggcaag
     4561 ggcagcaccg acgacgaagc tgaggacggc agggccgcgc ccgggccccg tgccacccca
     4621 ctgcggcggg ccgagtccct ggacccacgg cccctgcggc cggccgccct cccgcctacc
     4681 aagtgccgcg atcgcgacgg gcaggtggtg gccctgccca gcgacttctt cctgcgcatc
     4741 gacagccacc gtgaggatgc agccgagctt gacgacgact cggaggacag ctgctgcctc
     4801 cgcctgcata aagtgctgga gccctacaag ccccagtggt gccggagccg cgaggcctgg
     4861 gccctctacc tcttctcccc acagaaccgg ttccgcgtct cctgccagaa ggtcatcaca
     4921 cacaagatgt ttgatcacgt ggtcctcgtc ttcatcttcc tcaactgcgt caccatcgcc
     4981 ctggagaggc ctgacattga tcccggcagc accgagcggg tcttcctcag cgtctccaat
     5041 tacatcttca cggccatctt cgtggcggag atgatggtga aggtggtggc cctggggctg
     5101 ctgtccggcg agcacgccta cctgcagagc agctggaacc tgctggatgg gctgctggtg
     5161 ctggtgtccc tggtggacat tgtcgtggcc atggcctcgg ctggtggcgc caagatcctg
     5221 ggtgttctgc gcgtgctgcg tctgctgcgg accctgcggc ctctgagggt catcagccgg
     5281 gccccgggcc tcaagctggt ggtggagacg ctgatatcat cactcaggcc cattgggaac
     5341 atcgtcctca tctgctgcgc cttcttcatc atttttggca ttttgggtgt gcagctcttc
     5401 aaagggaagt tctactactg cgagggcccc gacaccagga acatctccac caaggcacag
     5461 tgccgggccg cccactaccg ctgggtgcga cgcaagtaca acttcgacaa cctgggccag
     5521 gccctgatgt cgctgttcgt gctgtcatcc aaggatggat gggtgaacat catgtacgac
     5581 gggctggatg ccgtgggtgt cgaccagcag cctgtgcaga accacaaccc ctggatgctg
     5641 ctgtacttca tctccttcct gctcatcgtc agcttcttcg tgctcaacat gttcgtgggc
     5701 gtcgtggtcg agaacttcca caagtgccgg cagcaccagg aggcggagga ggcgcggcgg
     5761 cgagaggaga agcggctgcg gcgcctagag aggaggcgca ggagcacttt ccccagccca
     5821 gaggcccagc gccggcccta ctatgccgac tactcgccca cgcgccgctc cattcactcg
     5881 ctgtgcacca gccactatct cgacctcttc atcaccttca tcatctgtgt caacgtcatc
     5941 accatgtcca tggagcacta taaccaaccc aagtcgctgg acgaggccct caagtactgc
     6001 aactacgtct tcaccatcgt gtttgtcttc gaggctgcac tgaagctggt agcatttggg
     6061 ttccgtcggt tcttcaagga caggtggaac cagctggacc tggccatcgt gctgctgtca
     6121 ctcatgggca tcacgctgga ggagatagag atgagcgccg cgctgcccat caaccccacc
     6181 atcatccgca tcatgcgcgt gcttcgcatt gcccgtgtgc tgaagctgct gaagatggct
     6241 acgggcatgc gcgccctgct ggacactgtg gtgcaagctc tcccccaggt ggggaacctg
     6301 ggccttcttt tcatgctcct gttttttatc tatgctgcgc tgggagtgga gctgttcggg
     6361 aggctggagt gcagtgaaga caacccctgc gagggcctga gcaggcacgc caccttcagc
     6421 aacttcggca tggccttcct cacgctgttc cgcgtgtcca cgggggacaa ctggaacggg
     6481 atcatgaagg acacgctgcg cgagtgctcc cgtgaggaca agcactgcct gagctacctg
     6541 ccggccctgt cgcccgtcta cttcgtgacc ttcgtgctgg tggcccagtt cgtgctggtg
     6601 aacgtggtgg tggccgtgct catgaagcac ctggaggaga gcaacaagga ggcacgggag
     6661 gatgcggagc tggacgccga gatcgagctg gagatggcgc agggccccgg gagtgcacgc
     6721 cgggtggacg cggacaggcc tcccttgccc caggagagtc cgggcgccag ggatgcccca
     6781 aacctggttg cacgcaaggt gtccgtgtcc aggatgctct cgctgcccaa cgacagctac
     6841 atgttcaggc ccgtggtgcc tgcctcggcg ccccaccccc gcccgctgca ggaggtggag
     6901 atggagacct atggggccgg cacccccttg ggctccgttg cctctgtgca ctctccgccc
     6961 gcagagtcct gtgcctccct ccagatccca ctggctgtgt cgtccccagc caggagcggc
     7021 gagcccctcc acgccctgtc ccctcggggc acagcccgct cccccagtct cagccggctg
     7081 ctctgcagac aggaggctgt gcacaccgat tccttggaag ggaagattga cagccctagg
     7141 gacaccctgg atcctgcaga gcctggtgag aaaaccccgg tgaggccggt gacccagggg
     7201 ggctccctgc agtccccacc acgctcccca cggcccgcca gcgtccgcac tcgtaagcat
     7261 accttcggac agcgctgcgt ctccagccgg ccggcggccc caggcggaga ggaggccgag
     7321 gcctcggacc cagccgacga ggaggtcagc cacatcacca gctccgcctg cccctggcag
     7381 cccacagccg agccccatgg ccccgaagcc tctccggtgg ccggcggcga gcgggacctg
     7441 cgcaggctct acagcgtgga tgctcagggc ttcctggaca agccgggccg ggcagacgag
     7501 cagtggcggc cctcggcgga gctgggcagc ggggagcctg gggaggcgaa ggcctggggc
     7561 cctgaggccg agcccgctct gggtgcgcgc agaaagaaga agatgagccc cccctgcatc
     7621 tcggtggaac cccctgcgga ggacgagggc tctgcgcggc cctccgcggc agagggcggc
     7681 agcaccacac tgaggcgcag gaccccgtcc tgtgaggcca cgcctcacag ggactccctg
     7741 gagcccacag agggctcagg cgccgggggg gaccctgcag ccaaggggga gcgctggggc
     7801 caggcctcct gccgggctga gcacctgacc gtccccagct ttgcctttga gccgctggac
     7861 ctcggggtcc ccagtggaga ccctttcttg gacggtagcc acagtgtgac cccagaatcc
     7921 agagcttcct cttcaggggc catagtgccc ctggaacccc cagaatcaga gcctcccatg
     7981 cccgtcggtg accccccaga gaagaggcgg gggctgtacc tcacagtccc ccagtgtcct
     8041 ctggagaaac cagggtcccc ctcagccacc cctgccccag ggggtggtgc agatgacccc
     8101 gtgtagctcg gggcttggtg ccgcccacgg ctttggccct ggggtctggg ggccccgctg
     8161 gggtggaggc ccaggcagaa ccctgcatgg accctgactt gggtcccgtc gtgagcagaa
     8221 aggcccgggg aggatgacgg cccaggccct ggttctctgc ccagcgaagc aggagtagct
     8281 gccgggcccc acgagcctcc gtccgttctg gttcgggttt ctccgagttt tgctaccagc
     8341 cgaggctgtg cgggcaactg ggtcagcctc ccgtcaggag agaagccgcg tctgtgggac
     8401 gaagaccggg cacccgccag agaggggaag gtaccaggtt gcgtcctttc aggccccgcg
     8461 ttgttacagg acactcgctg ggggccctgt gcccttgccg gcggcaggtt gcagccaccg
     8521 cggcccaatg tcaccttcac tcacagtctg agttcttgtc cgcctgtcac gccctcacca
     8581 ccctcccctt ccagccacca ccctttccgt tccgctcggg ccttcccaga agcgtcctgt
     8641 gactctggga gaggtgacac ctcactaagg ggccgacccc atggagtaac gcgcccggcc
     8701 ccgatgcgaa tcaggcctcc cctccggaat tcgagctcgg tacccgggga tcctctagag
     8761 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
     8821 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     8881 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     8941 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     9001 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     9061 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     9121 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9181 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9241 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9301 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9361 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9421 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9481 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     9541 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     9601 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     9661 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     9721 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     9781 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     9841 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     9901 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     9961 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
    10021 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
    10081 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
    10141 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
    10201 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
    10261 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
    10321 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
    10381 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
    10441 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
    10501 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
    10561 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
    10621 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
    10681 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
    10741 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
    10801 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    10861 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
    10921 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10981 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
    11041 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    11101 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    11161 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    11221 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    11281 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    11341 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    11401 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    11461 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    11521 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    11581 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    11641 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11701 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11761 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11821 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11881 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    11941 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    12001 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    12061 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    12121 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    12181 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    12241 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    12301 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    12361 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    12421 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    12481 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    12541 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    12601 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    12661 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    12721 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    12781 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    12841 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    12901 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    12961 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    13021 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    13081 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    13141 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    13201 agtgccacct gacgtc
//
