LOCUS       sequence-246440-        9582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral vector for expression of mutant G protein 
            alpha q in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9582)
  TITLE     Mariotti lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psd44-GqQL
FEATURES             Location/Qualifiers
     source          1..9582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(1931..1951)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1936..2265
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2116..2251
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2275..2874
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2275..2294)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2771..2791
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     promoter        2973..3483
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3402..3420
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    4650..5238
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4703..4723)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5121..5132)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5325..5558
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5630..5751
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5667..5686)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5721..5740
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5791..5926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5946..5965)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5947..5965)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5975..5992)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5975..5991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5984..6006)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6133..6588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6220..6239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6430..6451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6614..6718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6719..6787,6788..7579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6937..6956)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7750..8338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8239..8258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8492..8509
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8626..8647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8662..8667,8668..8685,8686..8692)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     mobile_element  8693..9460
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     protein_bind    9477..9493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9482..9504
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9501..9517
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9501..9517
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     1921 tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     1981 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     2041 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     2101 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     2161 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     2221 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct taccatgacc
     2281 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     2341 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     2401 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     2461 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     2521 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     2581 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     2641 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     2701 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     2761 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     2821 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     2881 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gatcacgaga
     2941 ctagcctcga gaagcttgat atcgaattcc acggggttgg ggttgcgcct tttccaaggc
     3001 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     3061 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     3121 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     3181 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     3241 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     3301 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     3361 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     3421 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     3481 cagggggatc ccgggcccgc ggtaccacca tgactctgga gtccatcatg gcgtgctgcc
     3541 tgagcgagga ggccaaggaa gcccggcgga tcaacgacga gatcgagcgg cagctccgca
     3601 gggacaagcg ggacgcccgc cgggagctca agctgctgct gctcgggaca ggagagagtg
     3661 gcaagagtac gtttatcaag cagatgagaa tcatccatgg gtcaggatac tctgatgaag
     3721 ataaaagggg cttcaccaag ctggtgtatc agaacatctt cacggccatg caggccatga
     3781 tcagagccat ggacacactc aagatcccat acaagtatga gcacaataag gctcatgcac
     3841 aattagttcg agaagttgat gtggagaagg tgtctgcttt tgagaatcca tatgtagatg
     3901 caataaagag tttatggaat gatcctggaa tccaggaatg ctatgataga cgacgagaat
     3961 atcaattatc tgactctacc aaatactatc ttaatgactt ggaccgcgta gctgaccctg
     4021 cctacctgcc tacgcaacaa gatgtgctta gagttcgagt ccccaccaca gggatcatcg
     4081 aatacccctt tgacttacaa agtgtcattt tcagaatggt cgatgtaggg ggcctaaggt
     4141 cagagagaag aaaatggata cactgctttg aaaatgtcac ctctatcatg tttctagtag
     4201 cgcttagtga atatgatcaa gttctcgtgg agtcagacaa tgagaaccga atggaggaaa
     4261 gcaaggctct ctttagaaca attatcacat acccctggtt ccagaactcc tcggttattc
     4321 tgttcttaaa caagaaagat cttctagagg agaaaatcat gtattcccat ctagtcgact
     4381 acttcccaga atatgatgga ccccagagag atgcccaggc agcccgagaa ttcattctga
     4441 agatgttcgt ggacctgaac ccagacagtg acaaaattat ctactcccac ttcacgtgcg
     4501 ccacagacac cgagaatatc cgctttgtct ttgctgccgt caaggacacc atcctccagt
     4561 tgaacctgaa ggagtacaat ctggtctaac tcgagatctg agtccggtag cgctagctga
     4621 tcacccgggg gccggcctgt acagtcgaca atcaacctct ggattacaaa atttgtgaaa
     4681 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4741 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4801 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4861 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4921 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4981 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5041 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5101 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5161 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5221 tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa
     5281 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5341 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5401 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5461 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5521 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5581 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     5641 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5701 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5761 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5821 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5881 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     5941 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     6001 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6061 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6121 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6181 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6241 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6301 ggttccgatt tagtgcatta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6361 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6421 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6481 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6541 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     6601 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6661 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6721 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6781 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6841 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6901 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6961 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7021 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7081 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7141 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7201 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7261 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7321 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7381 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7441 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7501 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7561 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7621 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7681 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7741 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7801 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7861 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7921 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7981 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8041 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8101 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8161 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8221 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8281 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8341 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8401 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8461 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8521 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     8581 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     8641 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgggtaatga
     8701 ctccaactta ttgatagtgt tttatgttca gataatgccc gatgactttg tcatgcagct
     8761 ccaccgattt tgagaacgac agcgacttcc gtcccagccg tgccaggtgc tgcctcagat
     8821 tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc agctttccct
     8881 tcaggcggga ttcatacagc ggccagccat ccgtcatcca tatcaccacg tcaaagggtg
     8941 acagcaggct cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa
     9001 caaccgtctt ccggagactg tcatacgcgt aaaacagcca gcgctggcgc gatttagccc
     9061 cgacatagcc ccactgttcg tccatttccg cgcagacgat gacgtcactg cccggctgta
     9121 tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc
     9181 aacgcccata atgcgggctg ttgcccggca tccaacgcca ttcatggcca tatcaatgat
     9241 tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac tgcagttgcc atgttttacg
     9301 gcagtgagag cagagatagc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc
     9361 gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa
     9421 acaccatcat acactaaatc agtaagttgg cagcatcacc cgtatgttgt gtggaattgt
     9481 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcgtt
     9541 taaactcact aaagggaaca aaagctggag ctgcaagctt aa
//
