LOCUS       sequence-246442-       15589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15589)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX026
FEATURES             Location/Qualifiers
     source          1..15589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5703..5727)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5756..5845
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5879..6131
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6560..6812)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7280..8305)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7280..8305)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8315..8659)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8801..8818)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8801..8817)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8810..8832)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8957..8976)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9167..9188
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9435..9459
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11055..12203)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12502..13296)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13488..14255
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14948..15579
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggcagc
     4621 agctatggat ttttggaata gttcatctgt agatgttcgc tcaacatcag aatctattat
     4681 tacttctggt ggtgaattaa tggaagcact tgaacctttt atgaaaagtg cttcttcttc
     4741 tccttctatt tctactcctt tcccttcttc tgattttcac tcttttcctt catttcctcc
     4801 tcctattcct tcccctactt ctaccatttc atatccttgc acttctttct accaaacagc
     4861 acctcaacct atgggctctg aatcttgttc cacttccaca actgaaaata cccaaatctt
     4921 ttcaactggg ttttcgggtt atggaatgga gcaactaggc tcaatcgggt tgaatcaatt
     4981 aaccccagct cagatccaac aaatccaagc ccaaatcaat ttccaaaccc aacaacaaca
     5041 acaacagatg atgttccata atggctccca ccaccatact tctactatga acatgttggg
     5101 cccaaagccc gttcaaatga agcaatcggg ctcaccacca aagcccacta agctctacag
     5161 gggcgtcaga caacgtcact ggggaaagtg ggtagctgag atccgattgc ccaagaaccg
     5221 gactcggctt tggcttggca ccttcgacac tgctgaagaa gctgcgttgg cctacgacaa
     5281 ggcggcgtat atgctacgtg gcgactttgc tagactcaac ttcccccatc tccgccataa
     5341 cggctccctt atcggcggcg aattcggaga gtacaatcca ttgcactcct ctgttgatgc
     5401 taagttaaag gagatttgcg agagcttggc ccagggaaag agcattgact ctaagaagaa
     5461 gaaggcgtcc aatgtgtctt cagcggccgc ggtggtgaaa atggaggaag aggagagcaa
     5521 ggtggccgaa ggtggatccg agggttccgg gtcgggttcc gggtcgggtg gatcatcgcc
     5581 cgtaactgac ctgacatttc cggagtttac tgaggaagag tcaacgtggg acatgtgttc
     5641 ggagaatatg ttgttgcaga agtatccatc tcatgagatt gattgggcct ctatacccgg
     5701 ggacccagct ttcttgtaca aagtggttga taacagcggg ttaattaaca tcttttaccc
     5761 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta
     5821 tccatatgac gttccagatt acgctgctca gtgcagcgct tagagctcga atttccccga
     5881 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     5941 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     6001 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     6061 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     6121 gttactagat cgggaattag cttcatcaac gcaagacatg cgcacgaccg tctgacagga
     6181 gaggaatttc cgacgagcac agaaaggact tgctcttgga cgtaggccta tttctcaggc
     6241 acatgtatca agtgttcgga cgtgggtttt cgatggtgta tcagccgccg ccaactggga
     6301 gatgaggagg ctttcttggg gggcagtcag cagttcattt cacaagacag aggaacttgt
     6361 aaggagatgc actgatttat cttggcgcaa accagcagga cgaattagtg ggaatagccc
     6421 gcgaatatct aagttatgcc tgtcggcatg agcagaaact tccaattcga aacagtttgg
     6481 agaggttgtt tttgggcata ccttttgtta gtcagcctct cgattgctca tcgtcattac
     6541 acagtaccga agtttgatcg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     6601 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     6661 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     6721 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     6781 aagaaacttt attgccaaat gtttgaacga tctgcttcga cgcactcctt ctttactcca
     6841 ccatctcgtc cttattgaaa acgtgggtag caccaaaacg aatcaagtcg ctggaactga
     6901 agttaccaat cacgctggat gatttgccag ttggattaat cttgcctttc cccgcatgaa
     6961 taatattgat gaatgcatgc gtgaggggta tttcgatttt ggcaatagct gcaattgccg
     7021 cgacatcctc caacgagcat aattcttcag aaaaatagcg atgttccatg ttgtcagggc
     7081 atgcatgatg cacgttatga ggtgacggtg ctaggcagta ttccctcaaa gtttcatagt
     7141 cagtatcata ttcatcattg cattcctgca agagagaatt gagacgcaat ccacacgctg
     7201 cggcaacctt ccggcgttcg tggtctattt gctcttggac gttgcaaacg taagtgttgg
     7261 atcccggtcg gcatctactc tattcctttg ccctcggacg agtgctgggg cgtcggtttc
     7321 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     7381 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     7441 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     7501 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga
     7561 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
     7621 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
     7681 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
     7741 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
     7801 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
     7861 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
     7921 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg
     7981 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
     8041 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg
     8101 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
     8161 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
     8221 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
     8281 tcgcggtgag ttcaggcttt ttcatatcgg ggtcgtcctc tccaaatgaa atgaacttcc
     8341 ttatatagag gaagggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt
     8401 ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     8461 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     8521 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     8581 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     8641 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     8701 ggagtagacg agagtgtcgt gctccaccat gttgacggat ctctaggacg cgtcgacgcg
     8761 tcgacgcgtc gacgcgtcga cgcgtcctag aagctaattc actggccgtc gttttacaac
     8821 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     8881 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     8941 gcctgaatgg cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9001 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9061 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     9121 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     9181 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg
     9241 ccgatttcgg aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
     9301 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
     9361 tggtgaaaag aaaaaccacc ccagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     9421 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     9481 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     9541 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     9601 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     9661 gattgtaacg atgacagagc gttgctgcct gtgatcaaat atcatctccc tcgcagagat
     9721 ccgaattatc agccttctta ttcatttctc gcttaaccgt gacaggctgt cgatcttgag
     9781 aactatgccg acataatagg aaatcgctgg ataaagccgc tgaggaagct gagtggcgct
     9841 atttctttag aagtgaacgt tgacgatatc aactccccta tccattgctc accgaatggt
     9901 acaggtcggg gacccgaagt tccgactgtc ggcctgatgc atccccggct gatcgacccc
     9961 agatctgggg ctgagaaagc ccagtaagga aacaactgta ggttcgagtc gcgagatccc
    10021 ccggaaccaa aggaagtagg ttaaacccgc tccgatcagg ccgagccacg ccaggccgag
    10081 aacattggtt cctgtaggca tcgggattgg cggatcaaac actaaagcta ctggaacgag
    10141 cagaagtcct ccggccgcca gttgccaggc ggtaaaggtg agcagaggca cgggaggttg
    10201 ccacttgcgg gtcagcacgg ttccgaacgc catggaaacc gcccccgcca ggcccgctgc
    10261 gacgccgaca ggatctagcg ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt
    10321 tccgcaaata gcccccagga ccgccatcaa tcgtatcggg ctacctagca gagcggcaga
    10381 gatgaacacg accatcagcg gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag
    10441 gcggtagacc gaaataaaca acaagctcca gaatagcgaa atattaagtg cgccgaggat
    10501 gaagatgcgc atccaccaga ttcccgttgg aatctgtcgg acgatcatca cgagcaataa
    10561 acccgccggc aacgcccgca gcagcatacc ggcgacccct cggcctcgct gttcgggctc
    10621 cacgaaaacg ccggacagat gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa
    10681 gaccgacagc ccaatgatct cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa
    10741 ccgttcagcg aacgcctcca tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat
    10801 ctctggagct ttcttcaggg ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc
    10861 tccaagccgt cgaatctgag ccttaatcac aattgtcaat tttaatcctc tgtttatcgg
    10921 cagttcgtag agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca
    10981 gtgcccgctt gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc agccggaact
    11041 gaccccacaa ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc
    11101 accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc
    11161 acgcgggtgg aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc
    11221 tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac
    11281 ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg
    11341 atcaggacct ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc
    11401 agcgacaccg attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt
    11461 aggcgcgaca ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg
    11521 tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg
    11581 tactccaaca cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg
    11641 taggtgatct tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc
    11701 gggattttct tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct
    11761 cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc
    11821 tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc
    11881 tcgccggcgg tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac
    11941 ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc
    12001 gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc
    12061 tggaccatcg agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt
    12121 gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc
    12181 ttccggtcaa acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg
    12241 gcaatgtgcc cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc
    12301 ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc
    12361 cgaatcttgc cctgcacgaa taccagcgac cccttgccca aatacttgcc gtgggcctcg
    12421 gcctgagagc caaaacactt gatgcggaag aagtcggtgc gctcctgctt gtcgccggca
    12481 tcgttgcgcc acatctaggt actaaaacaa ttcatccagt aaaatataat attttatttt
    12541 ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc
    12601 gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc
    12661 gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc
    12721 tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc
    12781 atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc
    12841 ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt
    12901 atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc
    12961 gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc
    13021 ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac
    13081 aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt
    13141 ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt
    13201 atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag
    13261 ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta
    13321 cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc
    13381 cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg
    13441 gcgtataaca tagtatcgac ggagccgatt ttgaaaccac aattatgggt gatgctgcca
    13501 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
    13561 agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga
    13621 catcagcgct atctctgctc tcactgccgt aaaacatggc aactgcagtt cacttacacc
    13681 gcttctcaac ccggtacgca ccagaaaatc attgatatgg ccatgaatgg cgttggatgc
    13741 cgggcaacag cccgcattat gggcgttggc ctcaacacga ttttacgtca cttaaaaaac
    13801 tcaggccgca gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg
    13861 aaatggacga acagtggggc tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg
    13921 cgtatgacag tctccggaag acggttgttg cgcacgtatt cggtgaacgc actatggcga
    13981 cgctggggcg tcttatgagc ctgctgtcac cctttgacgt ggtgatatgg atgacggatg
    14041 gctggccgct gtatgaatcc cgcctgaagg gaaagctgca cgtaatcagc aagcgatata
    14101 cgcagcgaat tgagcggcat aacctgaatc tgaggcagca cctggcacgg ctgggacgga
    14161 agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg cattatctga
    14221 acataaaaca ctatcaataa gttggagtca ttacccaatt atgatagaat ttacaagcta
    14281 taaggttatt gtcctgggtt tcaagcatta gtccatgcaa gtttttatgc tttgcccatt
    14341 ctatagatat attgataagc gcgctgccta tgccttgccc cctgaaatcc ttacatacgg
    14401 cgatatcttc tatataaaag atatattatc ttatcagtat tgtcaatata ttcaaggcaa
    14461 tctgcctcct catcctcttc atcctcttcg tcttggtagc tttttaaata tggcgcttca
    14521 tagagtaatt ctgtaaaggt ccaattctcg ttttcatacc tcggtataat cttacctatc
    14581 acctcaaatg gttcgctggg tttatcgcac ccccgaacac gagcacggca cccgcgacca
    14641 ctatgccaag aatgcccaag gtaaaaattg ccggccccgc catgaagtcc gtgaatgccc
    14701 cgacggccga agtgaagggc aggccgccac ccaggccgcc gccctcactg cccggcacct
    14761 ggtcgctgaa tgtcgatgcc agcacctgcg gcacgtcaat gcttccgggc gtcgcgctcg
    14821 ggctgatcgc ccatcccgtt actgccccga tcccggcaat ggcaaggact gccagcgctg
    14881 ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag cccctggggg gatgggaggc
    14941 ccgcgttagc gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt
    15001 cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca
    15061 ggttaaaaga caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat
    15121 tttctgcctg tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc
    15181 tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt
    15241 caataccgca gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa
    15301 aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga
    15361 gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc
    15421 gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggccg
    15481 cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc
    15541 gccagcccag cggcgagggc aaccagcccg gtgagcgtcg gaaaggcgc
//
