LOCUS       sequence-246443-       16930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16930)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX027
FEATURES             Location/Qualifiers
     source          1..16930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5167..5176
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             6001..6009
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(7044..7068)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7097..7186
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      7220..7472
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7901..8153)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8621..9646)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8621..9646)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9656..10000)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10142..10159)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10142..10158)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10151..10173)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10298..10317)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10508..10529
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10776..10800
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12396..13544)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13843..14637)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14829..15596
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16289..16920
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg gaaattgatc tgaaccatgc
     4621 agtagtaagt gaagtggaga agaatgcatg ttgtaatgga gaatgtgaca aaaagggagt
     4681 tggtggtagt tgtgttaatt gcaatttgtc cactacttct acttcttctt gttcctcaaa
     4741 tgcatcttcc tcttcttctt cttcacttac tctttcctca atttatatgg agctttggca
     4801 tgcatgtgct ggacctttaa caagcttacc aaagaaagga aatgtagtgg tttatttccc
     4861 tcaaggtcat atggaggaag ctgtttctgt ttcccctttt tcacccatca aaatggattt
     4921 tccaactttt ggtcttcaac ctcagatctt ctgtaaagtg gaagatgttc agttgcttgc
     4981 aaataaggaa aatgatgagg tttatacaca gcttactctt ctccctcttt cagagtcagt
     5041 ggctataagc ttaaagggaa aagaacatga agattttggg gtggatgagg aagggaatgg
     5101 agttaatcca ggaaaatcag cttctcacat gttctgtaag acgctgaccg cctctgatac
     5161 cagtacccat ggtggctttt cagttccccg tagagcagct gaagactgtt tcccacctct
     5221 ggattacaaa gagcaaaggc catctcaaga gctgattgct aaggatctgc acggagtaga
     5281 gtggaaattc cgtcacattt atagaggcca gccgaggcga catttgctaa ctactggatg
     5341 gagtatattt gtcagccaaa agaatcttgt ctcaggggat gcagttctct tcttgagggg
     5401 agaaggtgga gatctcaggc tgggaataag aagagctgct agacctagaa atggtcttcc
     5461 tgaatcaata atcaaaactc aatattctgg ttctgatgtt ctttcagcgg ttgctacagc
     5521 tgtgtcagtc aagagcacat tccatgtttt ctatagccca agggcaagtc atgctgattt
     5581 tgttgtaccg taccaaaagt acatgaaaag catcaacagt cggattcctg tccggacaag
     5641 atttaaaatg agattcgatt tggacgattc acctgaaaga aggtacagcg gtgtagtgac
     5701 cgggataggt gatatggatc cctttagatg gcccaactca aaatggagat gtttgatggt
     5761 cagatgggat gaagatatca tgagtaatca tcaagaacgg gtttctccat gggaaattga
     5821 ttcttcagtt tccttaccac caatgagcat tcagtctcca agactgaaga aactgcggac
     5881 tagtcagcag gctccatcct tagacagcca ttttgctgga gggaatgctc ttttggactt
     5941 tgaggagttg gtaagatcct ccaaggtctt gcaaggtcaa gaaaatttag gtctgatatc
     6001 acctccttat ggatgtgata agacagtccg cccactggat attgagctac aaaatgcagc
     6061 tcgtcacaat ctcatgccaa ctggtatgga gaacataatt gttggagact ttgtgaaaac
     6121 tcagcctcca actacctaca caggctttct ggaatccaat aggtttccaa aggtcttgca
     6181 aggtcaagaa atttgctcgt taagatcgct tactggaaaa ggtgatgtta attttggtgc
     6241 ttgggaaaaa cctgaatttg gttgcaatgt ttttagcaca tatcagaggc cgaagaccaa
     6301 tttctatcct cttgcttctg aaggggcgag gaatgtgttt ctaccttata atgcgatgta
     6361 tagagctgga caagatccag tagtgcattc ctacattaat aataatttcc aaagagaaaa
     6421 ccctaccttg aaccagaatt caatccaaaa tggggttagg agggaagaag gtggaatgca
     6481 gaagtttgca aatgagcaga ggccacctga gatgtccaaa ttgtctattc cagaaacaaa
     6541 tttcaagaat gagaatggta gctccttaaa tgcacaggct tcttgtaaac tatttggctt
     6601 ttccttaata aaagaaccct ctactcctag ctcacacagt tctggtaaga ggagctgtac
     6661 aaaggttcac aaacaaggca gcttggttgg acgagccatt gatctctcaa ggttgaatgg
     6721 ctatgaggat ttgctggttg agttggagag gcttttcaac atggaagacc tcctaagaga
     6781 tcccaataag ggatggcgaa tcctgtatac cgacagtgag aatgacatga tggttgtcgg
     6841 agacgatcca tggcatgagt tctgtgaggt agtatccaag atccatatat acactcaaga
     6901 agaagtggag aaaatgacta ttgaggggat aagtgatgat actcaaagtt gtttggagga
     6961 ggcgccggca ttcatggatg cctcaaaatc ttcttcagtt ggccagcctg attcttctcc
     7021 aactgcaatc aggatacccg gggacccagc tttcttgtac aaagtggttg ataacagcgg
     7081 gttaattaac atcttttacc catacgatgt tcctgactat gcgggctatc cctatgacgt
     7141 cccggactat gcaggatcct atccatatga cgttccagat tacgctgctc agtgcagcgc
     7201 ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     7261 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     7321 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     7381 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     7441 atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta gcttcatcaa cgcaagacat
     7501 gcgcacgacc gtctgacagg agaggaattt ccgacgagca cagaaaggac ttgctcttgg
     7561 acgtaggcct atttctcagg cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt
     7621 atcagccgcc gccaactggg agatgaggag gctttcttgg ggggcagtca gcagttcatt
     7681 tcacaagaca gaggaacttg taaggagatg cactgattta tcttggcgca aaccagcagg
     7741 acgaattagt gggaatagcc cgcgaatatc taagttatgc ctgtcggcat gagcagaaac
     7801 ttccaattcg aaacagtttg gagaggttgt ttttgggcat accttttgtt agtcagcctc
     7861 tcgattgctc atcgtcatta cacagtaccg aagtttgatc gatctagtaa catagatgac
     7921 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
     7981 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
     8041 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
     8101 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttcg
     8161 acgcactcct tctttactcc accatctcgt ccttattgaa aacgtgggta gcaccaaaac
     8221 gaatcaagtc gctggaactg aagttaccaa tcacgctgga tgatttgcca gttggattaa
     8281 tcttgccttt ccccgcatga ataatattga tgaatgcatg cgtgaggggt atttcgattt
     8341 tggcaatagc tgcaattgcc gcgacatcct ccaacgagca taattcttca gaaaaatagc
     8401 gatgttccat gttgtcaggg catgcatgat gcacgttatg aggtgacggt gctaggcagt
     8461 attccctcaa agtttcatag tcagtatcat attcatcatt gcattcctgc aagagagaat
     8521 tgagacgcaa tccacacgct gcggcaacct tccggcgttc gtggtctatt tgctcttgga
     8581 cgttgcaaac gtaagtgttg gatcccggtc ggcatctact ctattccttt gccctcggac
     8641 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     8701 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     8761 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     8821 gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa
     8881 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     8941 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     9001 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     9061 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     9121 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     9181 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     9241 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg
     9301 cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     9361 gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt
     9421 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     9481 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     9541 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     9601 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct
     9661 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     9721 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     9781 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     9841 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     9901 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     9961 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
    10021 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttgacgga
    10081 tctctaggac gcgtcgacgc gtcgacgcgt cgacgcgtcg acgcgtccta gaagctaatt
    10141 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    10201 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    10261 gcccttccca acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc
    10321 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
    10381 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
    10441 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
    10501 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
    10561 tttgatttat aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc
    10621 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
    10681 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc
    10741 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
    10801 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
    10861 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
    10921 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
    10981 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa
    11041 tatcatctcc ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg
    11101 tgacaggctg tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg
    11161 ctgaggaagc tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct
    11221 atccattgct caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg
    11281 catccccggc tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt
    11341 aggttcgagt cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag
    11401 gccgagccac gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa
    11461 cactaaagct actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt
    11521 gagcagaggc acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac
    11581 cgcccccgcc aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac
    11641 caacagcgcc acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg
    11701 gctacctagc agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt
    11761 cgccgcgacc ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga
    11821 aatattaagt gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg
    11881 gacgatcatc acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc
    11941 tcggcctcgc tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt
    12001 ggggccgtcc tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc
    12061 gaatgccacg gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg
    12121 ctcgtaaacg gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg
    12181 gaaatcctgc acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa
    12241 ttttaatcct ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga
    12301 gcgaagcaag tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct
    12361 gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca
    12421 tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg
    12481 acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt
    12541 tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc
    12601 gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac
    12661 agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc
    12721 aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg
    12781 aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg
    12841 ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg
    12901 ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg
    12961 gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc
    13021 ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg
    13081 gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa
    13141 agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg
    13201 ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct
    13261 cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa
    13321 cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc
    13381 tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc
    13441 gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg
    13501 atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg
    13561 attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc
    13621 ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg
    13681 tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc
    13741 aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg
    13801 cgctcctgct tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag
    13861 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
    13921 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
    13981 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
    14041 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
    14101 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
    14161 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
    14221 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
    14281 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
    14341 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
    14401 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    14461 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    14521 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    14581 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    14641 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    14701 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    14761 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca
    14821 caattatggg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
    14881 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
    14941 aacggcaaaa gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg
    15001 caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg
    15061 gccatgaatg gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg
    15121 attttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca
    15181 gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc
    15241 gccagcgctg gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat
    15301 tcggtgaacg cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg
    15361 tggtgatatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc
    15421 acgtaatcag caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc
    15481 acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca
    15541 aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc attacccaat
    15601 tatgatagaa tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca
    15661 agtttttatg ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc
    15721 ccctgaaatc cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta
    15781 ttgtcaatat attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag
    15841 ctttttaaat atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac
    15901 ctcggtataa tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca
    15961 cgagcacggc acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg
    16021 ccatgaagtc cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc
    16081 cgccctcact gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa
    16141 tgcttccggg cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa
    16201 tggcaaggac tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca
    16261 gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca
    16321 ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt
    16381 ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg
    16441 aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc
    16501 gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca
    16561 gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc
    16621 atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc
    16681 cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg
    16741 gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg
    16801 tatccacaac gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg
    16861 tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc
    16921 ggaaaggcgc
//
