LOCUS       sequence-246466-        9528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminal V5-tagged mouse Trpt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Unlu I, Lu Y, Wang X
  TITLE     The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune 
            noncanonical XBP1 splicing during ER stress.
  JOURNAL   J Biol Chem. 2018 Dec 14;293(50):19365-19376. doi: 
            10.1074/jbc.RA118.004872. Epub 2018 Oct 24.
  PUBMED    30355738
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004872"; journalName: "J Biol Chem"; date: 
            "2018-12-14- 14"; volume: "293"; issue: "50"; pages: "19365-19376"
COMMENT     Sequence Label: pB-puro-V5-Trpt1
FEATURES             Location/Qualifiers
     source          1..9528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          123..1139
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1063..1085
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1147..1166
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1201..1242
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    2027..2251
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2624..2686
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2752..2771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2753..2771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2778..2795)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2778..2794)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2787..2809)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2936..3391
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3023..3042)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3233..3254
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3417..3521
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3522..3590,3591..4382)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3740..3759)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4553..5141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5042..5061
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5295..5312
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5429..5450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5465..5470,5471..5488,5489..5495)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5503..5519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5508..5530
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5527..5543
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5527..5543
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5564..5582
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   5731..5765
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    7226..7259
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    7581..7805
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(7833..8435)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7916..7936)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     regulatory      8432..8441
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8455..8954)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8503..8522)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8896..8919
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    8985..9018
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        9032..9411
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggggsggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
       61 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      121 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      181 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      241 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      301 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      361 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      421 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
      481 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
      541 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
      601 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
      661 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
      721 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
      781 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
      841 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
      901 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
      961 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1021 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1081 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1141 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttggatcatg
     1201 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgggaattca aatgaacgcc
     1261 cctggaggaa ggaggaagga aggtagaagg actcatagac cccgggaaca ggaccgaaat
     1321 gtgcagctgt ctaaggctct gtcctatgcc cttcgccacg gggccttgaa gctgggactt
     1381 cccatgcgag ctgatggctt tgtgcccctg caagccctcc tgcagctgcc ccagttccac
     1441 agcttttcca ttgaagatgt gcagctcgtg gtcaatacca acgaaaagca gcggtttacc
     1501 ctgcagccag gcgagccgag cactgggctc ctcatcaggg ccaatcaggg ccactcccta
     1561 caagtccctg agttggaact gacgcccctg gagacaccac aagccctgcc cttgacacta
     1621 gtccatggta cattctggaa gcactggcca tctattctac taaagggctt gtcacgccaa
     1681 ggaaggacac acatccactt ggcctcagga ttgcctgggg accctggtgt catcagcggc
     1741 attcgtccaa attgtgaggt ggcggtgttc atcgatggac ccctagccct cacagatgga
     1801 atccctttct tctgctccgc caatggtgtg attctgactc cagggaatgc tgaaggcttc
     1861 ctgcttccca agtacttcaa ggaggccctg cagcttcgac ctacacgaaa gcctctctcc
     1921 ttggctggtg ataaagagac agagactcag agtggcccca agctcagttc cagaggagga
     1981 agaagaaaga tccaacaata actcgagggc cgctgatcag cctcgactgt gccttctagt
     2041 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     2101 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     2161 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     2221 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     2281 ttaattaacg agagcataat attgatatgt gccaaagttg tttctgactg actaataagt
     2341 ataatttgtt tctattatgt ataggttaag ctaattactt attttataat acaacatgac
     2401 tgtttttaaa gtacaaaata agtttatttt tgtaaaagag agaatgttta aaagttttgt
     2461 tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata aacataaata
     2521 aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac ttaatatcta
     2581 ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt caattttacg
     2641 catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa ataatagttt
     2701 ctaatttttt tattattcag cctgctgtcg tgaataccga gctccaattc gccctatagt
     2761 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     2821 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     2881 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     2941 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3001 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3061 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3121 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3181 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3241 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3301 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3361 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     3421 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3481 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3541 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3601 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3661 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3721 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     3781 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     3841 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     3901 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     3961 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4021 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4081 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4141 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4201 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4261 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4321 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4381 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4441 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4501 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4561 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4621 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4681 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4741 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4801 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4861 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4921 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     4981 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5041 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5161 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5221 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5281 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5341 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     5401 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     5461 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     5521 ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac cctcactaaa
     5581 gggaacaaaa gctggtacct cgcgcgactt ggtttgccat tctttagcgc gcgtcgcgtc
     5641 acacagcttg gccacaatgt ggtttttgtc aaacgaagat tctatgacgt gtttaaagtt
     5701 taggtcgagt aaagcgcaaa tcttttttaa ccctagaaag atagtctgcg taaaattgac
     5761 gcatgcattc ttgaaatatt gctctctctt tctaaatagc gcgaatccgt cgctgtgcat
     5821 ttaggacatc tcagtcgccg cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt
     5881 gtcactgatt ttgaactata acgaccgcgt gagtcaaaat gacgcatgat tatcttttac
     5941 gtgactttta agatttaact catacgataa ttatattgtt atttcatgtt ctacttacgt
     6001 gataacttat tatatatata ttttcttgtt atagatatcg tgactaatat ataataaaat
     6061 gggtagttct ttagacgatg agcatatcct ctctgctctt ctgcaaagcg atgacgagct
     6121 tgttggctag catcgagcaa gacgttcagt cctacagaaa taaaatcagg aatttaatag
     6181 aaagtttcat acattaaact ttataacaaa cacctcttag tcattaaact tccacaccaa
     6241 cctgggcaat atagtgagac cccatgcctg caaaaaaaaa aaaattagcc aggcatggta
     6301 gcatgtacct gtagtcccag ctacttgaga ggtgaggtgg gaaaatcact ttagtgcagg
     6361 atgttgaggc tggagtgaac tgtgattgtg ccactgcact ccagcctgga caatagagca
     6421 agaccttgtc tcaaaaaaat gcattaaaaa ttttttttaa atcttccacg taacacatcc
     6481 tttgccctca tgtttcataa ggtaaaaaat ttgatacctt caaaaaaacc aagcatacca
     6541 ctatcataat tttttttaaa tgcaaataaa aacaagatac cattttcacc tatcagactg
     6601 gcaggttctg attaaatgaa atttcttgga taatatacaa tattaagaga gactgtagaa
     6661 actgggccag tggctcatgc ctgtaatccc agcactttgg gaggctgggt aacatggcga
     6721 accctgtttc tacaaaataa aaatattagc tgggagtggt ggcgcacacc tatagtccca
     6781 gctactcagg aggctgaggt ggaaggatcg cttgaaccca ggaggttgag actgcagtga
     6841 actgtgatca ttctgctgca ctgcacccca gcctgggcaa cagagacctt gtctcaaaaa
     6901 aaaaaaaaaa gagacaaatt gtgaagagaa aggtactctc atataacatc aggagtataa
     6961 aatgattcaa cttcttagag gaaaatttgg caataccaaa atattcaata aactctttcc
     7021 ccttgaccca gaaattccac ttgaataaag ctgaacaagt accaaacatg taaaagaatg
     7081 tttcttctag tacagtcggt aagaacaaaa tagtgtctat caatagtgga ctggttaaat
     7141 cagttatggt atctccataa gacagaatgc tatgcaacct ttaaaatata ttagatagct
     7201 ctagaattga tccggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt
     7261 aggtccctcg acctgcagcc caagctctag acacactaat attaaaagtg tccaataaca
     7321 tttaaaacta tactcatacg ttaaaatata aatgtatata tgtacttttg catatagtat
     7381 acatgcatag ccagtgcttg agaagaaatg tgtacagaag gctgaaagga gagaacttta
     7441 gtcttcttgt ttatggcctc catagttaga atattttata acacaaatat tttgatatta
     7501 taattttaaa ataaaaacac agaatagcca gacatacaat gcaagcgtcg agccccagct
     7561 ggttctttcc gcctcagaag ccatagagcc caccgcatcc ccagcatgcc tgctattgtc
     7621 ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct
     7681 actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc
     7741 ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg
     7801 cacagtcgag gctgatcagc gagctctaga gctcaggcac cgggcttgcg ggtcatgcac
     7861 caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg
     7921 tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg
     7981 gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc
     8041 gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg
     8101 ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg
     8161 atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg
     8221 gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt
     8281 tcttgcagct cggtgacccg ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg
     8341 gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg
     8401 gcgaggcgca ccgtgggctt gtactcggtc cccatggtgg cgttggctgc aggtcgaaag
     8461 gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa
     8521 tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc
     8581 gcccccggac ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta
     8641 ttggccgctg ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac
     8701 gagactagtg agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc
     8761 gggggggaac ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa
     8821 cggagccggt tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt
     8881 gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag
     8941 cgcctcccct acccggtaga attaattcga tatcggatcg atccataact tcgtataatg
     9001 tatgctatac gaagttatct cgatcgaggt cgacattgat tattgactag ttattaatag
     9061 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     9121 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     9181 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     9241 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     9301 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     9361 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
     9421 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
     9481 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggg
//
