LOCUS       sequence-246469-       16153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16153)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX028
FEATURES             Location/Qualifiers
     source          1..16153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6267..6291)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6320..6409
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6443..6695
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7124..7376)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7844..8869)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7844..8869)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8879..9223)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9365..9382)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9365..9381)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9374..9396)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9521..9540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9731..9752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9999..10023
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11619..12767)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13066..13860)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14052..14819
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15512..16143
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg cagaattggg gatgctctct
     4621 ttatggcatc ttagaacgaa ttaatcatgc tggtttaatg tgccaagatc ttccaatagc
     4681 tttttctgtc atggttttat tgggagattg tctggtctcc gatttacacc ttgcagctgt
     4741 tccaacagat tctgctgaaa taattttgaa aatacttagg cctgagtctc atcctgaatt
     4801 agcttattca tatctgttct gcctttcagc accttctatg agttaattct acacgtttta
     4861 tgtcatgtca ctcgattgaa atctgatttt caggattgct ttatcttgtc ataagtcgta
     4921 actaatctgc tctttcgttt ccctttctgg gagggatatt aaattttttt ttgtcaagtc
     4981 cggattattg taatctctgg acctctacct atttaacggc ataaatacat gtatttctat
     5041 catcatcaaa gcgttaaaaa tcttttgttc tttgctcgtc tctgctttct tacgtttctc
     5101 tcaaggtcat tggagctaat gatgagtgtt tgtctttcag gttctttatg attcagcctt
     5161 gattaagaga atgcagatct taatctgttg gtagttggga ttgactttcc ttgatgagta
     5221 tgtcaattta tcggattgaa ggctgcaatt gattttgacg ttaaccaccc aaatatatcg
     5281 tatggatgac agtgggcatc gtgataacgg aaggcataaa ccgcctcagg gtcagtggtt
     5341 gatgcagcat cagccatcaa tgaagcagat aatggcaatc atggcagaaa gagatgccgc
     5401 catacaagag cggaatctgg ccctttctga aaaaaaggct gccttggcag agcgtgacat
     5461 ggctatcctt cagagggatt cagcaatagc agaacgaaat aatgcaatca tggaacggga
     5521 taacgccatt gctactcttc agtatcggga aagctcgata agtggtggtg gtcagattgc
     5581 ccgtggagtg gtaaaacaca tgcaccatcc tcaacatgta catgtacata atcagccaca
     5641 tatgggagaa cctacatata acccgagagg catgcacgtg aatgaatcca taccggtttc
     5701 tcctgttgca ccggagccca caaagccccg tcggaacaaa agagctaagg agccaaaggc
     5761 aatgacgggt tccaagaaga ctccaaaagc atcaaagaaa gtaaaaaggg aaactgaaga
     5821 tcttaaccaa acgacatttg gcaaatcaca agagtggaag ggtgtacaag agatgggtga
     5881 tgcaagtgat gaccttaata ggcaattagc tgggtcaaaa cccgattgga aggatcagga
     5941 cctgggacta aaccaagtcg cgtttgatga aacaacaatg ccagtcccgg tttgttcttg
     6001 caccggagtt ttaagacctt gctataagtg gggaaacgga ggttggcagt cctcgtgttg
     6061 cacgaccaat ttgtcgatgt atccactacc agccgttcct aacaagcgac atgccagaat
     6121 tggtggtcgg aaaatgagtg gtagtgcgtt caccaagctg cttagtcgtc tggctgcaga
     6181 aggccatgac ctatcaaatc cagttgatct caagaaccac tgggcaaagc atggtacaaa
     6241 ccgctacatc accatcaaac ccggggaccc agctttcttg tacaaagtgg ttgataacag
     6301 cgggttaatt aacatctttt acccatacga tgttcctgac tatgcgggct atccctatga
     6361 cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg ctcagtgcag
     6421 cgcttagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     6481 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     6541 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     6601 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     6661 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttagcttcat caacgcaaga
     6721 catgcgcacg accgtctgac aggagaggaa tttccgacga gcacagaaag gacttgctct
     6781 tggacgtagg cctatttctc aggcacatgt atcaagtgtt cggacgtggg ttttcgatgg
     6841 tgtatcagcc gccgccaact gggagatgag gaggctttct tggggggcag tcagcagttc
     6901 atttcacaag acagaggaac ttgtaaggag atgcactgat ttatcttggc gcaaaccagc
     6961 aggacgaatt agtgggaata gcccgcgaat atctaagtta tgcctgtcgg catgagcaga
     7021 aacttccaat tcgaaacagt ttggagaggt tgtttttggg catacctttt gttagtcagc
     7081 ctctcgattg ctcatcgtca ttacacagta ccgaagtttg atcgatctag taacatagat
     7141 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
     7201 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
     7261 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
     7321 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatctgct
     7381 tcgacgcact ccttctttac tccaccatct cgtccttatt gaaaacgtgg gtagcaccaa
     7441 aacgaatcaa gtcgctggaa ctgaagttac caatcacgct ggatgatttg ccagttggat
     7501 taatcttgcc tttccccgca tgaataatat tgatgaatgc atgcgtgagg ggtatttcga
     7561 ttttggcaat agctgcaatt gccgcgacat cctccaacga gcataattct tcagaaaaat
     7621 agcgatgttc catgttgtca gggcatgcat gatgcacgtt atgaggtgac ggtgctaggc
     7681 agtattccct caaagtttca tagtcagtat catattcatc attgcattcc tgcaagagag
     7741 aattgagacg caatccacac gctgcggcaa ccttccggcg ttcgtggtct atttgctctt
     7801 ggacgttgca aacgtaagtg ttggatcccg gtcggcatct actctattcc tttgccctcg
     7861 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     7921 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     7981 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     8041 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     8101 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     8161 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     8221 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     8281 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     8341 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     8401 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     8461 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     8521 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     8581 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
     8641 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     8701 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     8761 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     8821 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tcggggtcgt
     8881 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     8941 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
     9001 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     9061 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
     9121 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
     9181 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
     9241 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttgac
     9301 ggatctctag gacgcgtcga cgcgtcgacg cgtcgacgcg tcgacgcgtc ctagaagcta
     9361 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     9421 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     9481 atcgcccttc ccaacagttg cgcagcctga atggcgcccg ctcctttcgc tttcttccct
     9541 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9601 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     9661 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9721 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     9781 tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag gattttcgcc
     9841 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
     9901 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccccagta cattaaaaac
     9961 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
    10021 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
    10081 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
    10141 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
    10201 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
    10261 aaatatcatc tccctcgcag agatccgaat tatcagcctt cttattcatt tctcgcttaa
    10321 ccgtgacagg ctgtcgatct tgagaactat gccgacataa taggaaatcg ctggataaag
    10381 ccgctgagga agctgagtgg cgctatttct ttagaagtga acgttgacga tatcaactcc
    10441 cctatccatt gctcaccgaa tggtacaggt cggggacccg aagttccgac tgtcggcctg
    10501 atgcatcccc ggctgatcga ccccagatct ggggctgaga aagcccagta aggaaacaac
    10561 tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac ccgctccgat
    10621 caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga ttggcggatc
    10681 aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc aggcggtaaa
    10741 ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga acgccatgga
    10801 aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa
    10861 caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca tcaatcgtat
    10921 cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca cagcgcctac
    10981 cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc tccagaatag
    11041 cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg ttggaatctg
    11101 tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcagca taccggcgac
    11161 ccctcggcct cgctgttcgg gctccacgaa aacgccggac agatgcgcct tgtgagcgtc
    11221 cttggggccg tcctcctgtt tgaagaccga cagcccaatg atctcgccgt cgatgtaggc
    11281 gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc tccatgggct ttttctcctc
    11341 gtgctcgtaa acggacccga acatctctgg agctttcttc agggccgaca atcggatctc
    11401 gcggaaatcc tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt
    11461 caattttaat cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac
    11521 tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag taaagcgctg
    11581 gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg
    11641 tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg
    11701 ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag
    11761 gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg
    11821 gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca
    11881 aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg
    11941 tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac
    12001 gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac
    12061 cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc
    12121 tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg
    12181 tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg
    12241 accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag
    12301 cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag
    12361 gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc
    12421 tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt
    12481 cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc
    12541 gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg
    12601 cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg
    12661 ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg
    12721 tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc
    12781 gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt
    12841 gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg
    12901 ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag cgaccccttg
    12961 cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg gaagaagtcg
    13021 gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa acaattcatc
    13081 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
    13141 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
    13201 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
    13261 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
    13321 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
    13381 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
    13441 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
    13501 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
    13561 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
    13621 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
    13681 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
    13741 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
    13801 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
    13861 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    13921 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    13981 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    14041 ccacaattat gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
    14101 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
    14161 cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg ccgtaaaaca
    14221 tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat
    14281 atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt tggcctcaac
    14341 acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacagccgg
    14401 gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggctatgtc ggggctaaat
    14461 cgcgccagcg ctggctgttt tacgcgtatg acagtctccg gaagacggtt gttgcgcacg
    14521 tattcggtga acgcactatg gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg
    14581 acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg aagggaaagc
    14641 tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg aatctgaggc
    14701 agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg
    14761 acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga gtcattaccc
    14821 aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc attagtccat
    14881 gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg cctatgcctt
    14941 gccccctgaa atccttacat acggcgatat cttctatata aaagatatat tatcttatca
    15001 gtattgtcaa tatattcaag gcaatctgcc tcctcatcct cttcatcctc ttcgtcttgg
    15061 tagcttttta aatatggcgc ttcatagagt aattctgtaa aggtccaatt ctcgttttca
    15121 tacctcggta taatcttacc tatcacctca aatggttcgc tgggtttatc gcacccccga
    15181 acacgagcac ggcacccgcg accactatgc caagaatgcc caaggtaaaa attgccggcc
    15241 ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg ccacccaggc
    15301 cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc tgcggcacgt
    15361 caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc ccgatcccgg
    15421 caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc
    15481 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
    15541 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
    15601 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
    15661 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
    15721 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
    15781 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
    15841 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
    15901 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
    15961 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
    16021 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
    16081 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
    16141 gtcggaaagg cgc
//
