LOCUS       sequence-24647-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  TITLE     Lowe Lab Plasmids
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tTA IRES GFP
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(141..160)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     gap             596..606
                     /estimated_length=11
ORIGIN
        1 cnncttnnnn nnccctcact ccttctctag gcgccggaat tagatctctc gaggttaacg
       61 aattcatatg tctagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa
      121 tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca
      181 gcctacattg tattggcatg taaaanataa gcgggctttg ctcgacgcct tagccattga
      241 gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt
      301 tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
      361 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
      421 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg
      481 gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag
      541 ggaaacacct actactgata gtatgccgcc attattacga caagctatcg aatttnnnnn
      601 nnnnnnaagg tgcagagcca ngncttnctt attcggcctt gaattgatca tatgcggatt
      661 agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc cgcgcgcgta cgaaaaacaa
      721 ttacgggtct accatcgagg gcctgctcga tctcccggga cgacgacgcc cccgaannnn
      781 nggggctggn ngcnncnnnn ccggnccttt cncccc
//
